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Rapid Epidemiologic Characterization of Cytomegalovirus Strains From Pediatric Bone Marrow Transplant Patients

Published online by Cambridge University Press:  02 January 2015

Inara E. Souza ,
Donald Nicholson ,
Sandra Matthey ,
Beth Alden ,
Thomas H. Haugen ,
Michael E. Trigg  and
James E Bale Jr
Inara E. Souza*
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa Department of Pediatrics at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Donald Nicholson
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Sandra Matthey
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Beth Alden
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Thomas H. Haugen
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Michael E. Trigg
Affiliation:
Department of Pediatrics at the University of Iowa Hospital and Clinics, Iowa City, Iowa
James E Bale Jr
Affiliation:
Department of Pediatrics at the University of Iowa Hospital and Clinics, Iowa City, Iowa
*
Escola Paulista de Medicine, Sao Paulo, Brazil
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Abstract

Background:

DNA amplification by the polymerase chain reaction (PCR) of human cytomegalovirus (CMV) nucleotide sequences recently has been reported for differentiation of CMV strains.

Design:

Retrospective study.

Objective and Patients:

Evaluate the strain patterns of 15 CMV-positive buffy coat specimens from five pediatric bone marrow transplant patients.

Setting:

Pediatric bone marrow transplant unit.

Methods:

We performed PCR using primers corresponding to two distinct regions of the CMV genome, the major immediate-early (MIE) region and the a-sequence region, with subsequent restriction enzyme analysis of the amplified products.

Results:

Restriction enzyme analysis with Hae III and Hinf I of products amplified with nested PCR for the MIE region revealed distinguishable digestion patterns between patients but similar patterns for samples from each patient. All were distinct from the CMV Towne laboratory control strain. In contrast to these results, amplification of specimens with a-sequence primers, followed by restriction enzyme analysis, did not allow differentiation between all patients.

Conclusion:

Our results indicate that nested amplification directly from buffy coat specimens using primers for the CMV MIE gene allows rapid CMV strain characterization that is useful for laboratory quality control and epidemiological studies. Distinct CMV strains were found in each patient, suggesting horizontal transmission was not responsible for acquisition of CMV infection in these patients.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 16 , Issue 7 , July 1995 , pp. 399 - 404
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1995

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References

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