Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-15T01:55:37.953Z Has data issue: false hasContentIssue false
  • English
  • Français

Ribotyping and Random Amplification of Polymorphic DNA for Nosocomial Enterobacter cloacae Isolates in a Pediatric Intensive-Care Unit

Published online by Cambridge University Press:  02 January 2015

Shih-Ting Hou ,
Chih-Chien Wang  and
Mong-Ling Chu
Get access Rights & Permissions [Opens in a new window]

Abstract

Between 1987 and 1989, two sequential outbreaks of nosocomial infection caused by Enterobacter cloacae occurred in the pediatric intensive-care unit of a tertiary-care teaching hospital. Seventeen strains retrieved from the outbreaks and two control strains identified in other wards were typed by ribotyping and random amplification of polymorphic DNA (RAPD). The results indicated that the genomic pattern of strains identified between the first and second outbreaks was different. We conclude that both ribotyping and RAPD are highly discriminatory and reproducible methods for typing E cloacae. RAPD seems to be more efficacious and cost-effective.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 18 , Issue 11 , November 1997 , pp. 769 - 771
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Haertl, R, Bandlow, G. Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonude-ase analysis and pulsed-field gel electrophoresis of genomic restrict & fragments. J Clin Microbiol 1993;31:128–133.CrossRefGoogle ScholarPubMed
2. Ni Riain, U. Cormican, MG. Flynn, J. Smith, T. Glennon, M. PCR based fingerprinting of Enterobacter cloacae . J Hosp Infect 1994;27:237–240.Google Scholar
3. Garaizar, J, Kaufmann, ME, Pitt, TL. Comparison of ribotyping with conventional methods for the type identification of Enterobacter cloacae . J Clin Microbiol 1991;29:1303–1307.Google Scholar
4. Andresen, J, Asmar, BI, Daj ani, AS. Increasing Enterobacter bacteremia in pediatric patients. Pediatr Infect Dis J 1994;13:787–792.Google Scholar
5. Bouza, E, Garcia de la Torre, M, Erice, A, et al. Enterobacter bacteremia: an analysis of 50 episodes. Arch Intern Med 1985;145:1024–1027,CrossRefGoogle ScholarPubMed
6. Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appi Microbiol 1989;8:151–156.CrossRefGoogle Scholar
7. Brosius, J, Ullrich, A, Raker, MA, et al. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA Operon of E coli. Plasmid 1951;6:112–118.Google Scholar
8. van Soolingen, D, Hermans, PWM, de Haas, PEW, Soll, DR van Embden, JDA. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol 1991;29:2578–2586.Google Scholar
9. Grattard, F, Pozzetto, B, Berthelot, P, et al. Arbitrarily primed PCR, ribotyping, and plasmid pattern analysis applied to investigation of nosocomial outbreak due to Enterobacter cloacae in a neonatal intensive care unit. J Clin Microbiol 1994;32:596–602.Google Scholar