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Strategies to Prevent Methicillin-Resistant Staphylococcus aureus Transmission and Infection in Acute Care Hospitals: 2014 Update

Part of: ICHE Compendia Collection 2014

Published online by Cambridge University Press:  10 May 2016

David P. Calfee ,
Cassandra D. Salgado ,
Aaron M. Milstone ,
Anthony D. Harris ,
David T. Kuhar ,
Julia Moody ,
Kathy Aureden ,
Susan S. Huang ,
Lisa L. Maragakis  and
Deborah S. Yokoe
David P. Calfee
Affiliation:
Weill Cornell Medical College, New York, New York
Cassandra D. Salgado
Affiliation:
Medical University of South Carolina, Charleston, South Carolina
Aaron M. Milstone
Affiliation:
Johns Hopkins University School of Medicine, Baltimore, Maryland
Anthony D. Harris
Affiliation:
University of Maryland School of Medicine, Baltimore, Maryland
David T. Kuhar
Affiliation:
Centers for Disease Control and Prevention, Atlanta, Georgia
Julia Moody
Affiliation:
Hospital Corporation of America, Nashville, Tennessee
Kathy Aureden
Affiliation:
Advocate Sherman Hospital, Elgin, Illinois
Susan S. Huang
Affiliation:
University of California, Irvine, School of Medicine, Irvine, California
Lisa L. Maragakis
Affiliation:
Johns Hopkins University School of Medicine, Baltimore, Maryland
Deborah S. Yokoe
Affiliation:
Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts
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Previously published guidelines are available that provide comprehensive recommendations for detecting and preventing healthcare-associated infections (HAIs). The intent of this document is to highlight practical recommendations in a concise format designed to assist acute care hospitals in implementing and prioritizing their methicillin-resistant Staphylococcus aureus (MRSA) prevention efforts. This document updates “Strategies to Prevent Transmission of Methicillin-Resistant Staphylococcus aureus in Acute Care Hospitals,” published in 2008. This expert guidance document is sponsored by the Society for Healthcare Epidemiology of America (SHEA) and is the product of a collaborative effort led by SHEA, the Infectious Diseases Society of America (IDSA), the American Hospital Association (AHA), the Association for Professionals in Infection Control and Epidemiology (APIC), and The Joint Commission, with major contributions from representatives of a number of organizations and societies with content expertise. The list of endorsing and supporting organizations is presented in the introduction to the 2014 updates.

Type
SHEA/IDSA Practice Recommendation
Information
Infection Control & Hospital Epidemiology , Volume 35 , Issue S2: A Compendium of Strategies to Prevent Healthcare-Associated Infections in Acute Care Hospitals: 2014 Updates , September 2014 , pp. S108 - S132
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2014

References

1. Calfee, DP, Salgado, CD, Classen, D, et al. Strategies to prevent transmission of methicillin-resistant Staphylococcus aureus in acute care hospitals. Infect Control Hosp Epidemiol 2008;29(suppl 1):S62–S80.CrossRefGoogle ScholarPubMed
2. Yokoe, DS, Anderson, DJ, Berenholtz, SM, et al. Introduction to “A Compendium of Strategies to Prevent Healthcare-Associated Infections in Acute Care Hospitals: 2014 Updates.” Infect Control Hosp Epidemiol 2014;35(5):455–459.Google Scholar
3. Sievert, DM, Ricks, P, Edwards, JR, et al. Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009-2010. Infect Control Hosp Epidemiol 2013;34:1–14.CrossRefGoogle Scholar
4. Hidron, AI, Edwards, JR, Patel, J, et al. NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007. Infect Control Hosp Epidemiol 2008;29:996–1011.CrossRefGoogle Scholar
5. Kallen, AJ, Mu, Y, Bulens, S, et al. Health care-associated invasive MRSA infections, 2005-2008. JAMA 2010;304:641–648.Google Scholar
6. Dantes, R, Mu, Y, Belflower, R, et al. National burden of invasive methicillin-resistant Staphylococcus aureus infections, United States, 2011. JAMA Intern Med 2013;173(21):1970–1978.Google Scholar
7. Iwamoto, M, Mu, Y, Lynfield, R, et al. Trends in invasive methicillin-resistant Staphylococcus aureus infections. Pediatrics 2013;132:e817–e824.CrossRefGoogle ScholarPubMed
8. Cosgrove, SE, Sakoulas, G, Perencevich, EN, Schwaber, MJ, Karchmer, AW, Carmeli, Y. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin Infect Dis 2003; 36:53–59.CrossRefGoogle ScholarPubMed
9. Engemann, JJ, Carmeli, Y, Cosgrove, SE, et al. Adverse clinical and economic outcomes attributable to methicillin resistance among patients with Staphylococcus aureus surgical site infection. Clin Infect Dis 2003;36:592–598.CrossRefGoogle ScholarPubMed
10. Abramson, MA, Sexton, DJ. Nosocomial methicillin-resistant and methicillin-susceptible Staphylococcus aureus primary bacteremia: at what costs? Infect Control Hosp Epidemiol 1999;20: 408–411.CrossRefGoogle ScholarPubMed
11. McHugh, CG, Riley, LW. Risk factors and costs associated with methicillin-resistant Staphylococcus aureus bloodstream infections. Infect Control Hosp Epidemiol 2004;25:425–430.CrossRefGoogle ScholarPubMed
12. Harbarth, S, Rutschmann, O, Sudre, P, Pittet, D. Impact of methicillin resistance on the outcome of patients with bacteremia caused by Staphylococcus aureus . Arch Intern Med 1998;158: 182–189.CrossRefGoogle ScholarPubMed
13. Cosgrove, SE, Qi, Y, Kaye, KS, Harbarth, S, Karchmer, AW, Carmeli, Y. The impact of methicillin resistance in Staphylococcus aureus bacteremia on patient outcomes: mortality, length of stay, and hospital charges. Infect Control Hosp Epidemiol 2005; 26:166–174.CrossRefGoogle ScholarPubMed
14. Huang, SS, Platt, R. Risk of methicillin-resistant Staphylococcus aureus infection after previous infection or colonization. Clin Infect Dis 2003;36:281–285.CrossRefGoogle ScholarPubMed
15. Garrouste-Orgeas, M, Timsit, JF, Kallel, H, et al. Colonization with methicillin-resistant Staphylococcus aureus in ICU patients: morbidity, mortality, and glycopeptide use. Infect Control Hosp Epidemiol 2001;22:687–692.Google Scholar
16. Freitas, EA, Harris, RM, Blake, RK, Salgado, CD. Prevalence of USA300 strain type of methicillin-resistant Staphylococcus aureus among patients with nasal colonization identified with active surveillance. Infect Control Hosp Epidemiol 2010;31:469–475.CrossRefGoogle ScholarPubMed
17. Huang, SS, Hinrichsen, VL, Datta, R, et al. Methicillin-resistant Staphylococcus aureus infection and hospitalization in high-risk patients in the year following detection. PLoS ONE 2011;6: e24340.Google ScholarPubMed
18. Datta, R, Huang, SS. Risk of infection and death due to methicillin-resistant Staphylococcus aureus in long-term carriers. Clin Infect Dis 2008;47:176–181.CrossRefGoogle ScholarPubMed
19. Milstone, AM, Goldner, BW, Ross, T, Shepard, JW, Carroll, KC, Perl, TM. Methicillin-resistant Staphylococcus aureus colonization and risk of subsequent infection in critically ill children: importance of preventing nosocomial methicillin-resistant Staphylococcus aureus transmission. Clin Infect Dis 2011;53: 853–859.CrossRefGoogle ScholarPubMed
20. Merrer, J, Santoli, F, Appere de Vecchi, C, Tran, B, De Jonghe, B, Outin, H. “Colonization pressure” and risk of acquisition of methicillin-resistant Staphylococcus aureus in a medical intensive care unit. Infect Control Hosp Epidemiol 2000;21:718–723.Google Scholar
21. Moran, GJ, Krishnadasan, A, Gorwitz, RJ, et al. Methicillin-resistant S. aureus infections among patients in the emergency department. N Engl J Med 2006;355:666–674.Google Scholar
22. King, MD, Humphrey, BJ, Wang, YF, Kourbatova, EV, Ray, SM, Blumberg, HM. Emergence of community-acquired methicillin-resistant Staphylococcus aureus USA 300 clone as the predominant cause of skin and soft-tissue infections. Ann Intern Med 2006;144:309–317.CrossRefGoogle ScholarPubMed
23. Maree, CL, Daum, RS, Boyle-Vavra, S, Matayoshi, K, Miller, LG. Community-associated methicillin-resistant Staphylococcus aureus isolates causing healthcare-associated infections. Emerg Infect Dis 2007;13:236–242.Google Scholar
24. Klevens, RM, Morrison, MA, Nadle, J, et al. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA 2007;298:1763–1771.Google Scholar
25. Popovich, KJ, Weinstein, RA, Hota, B. Are community-associated methicillin-resistant Staphylococcus aureus (MRSA) strains replacing traditional nosocomial MRSA strains? Clin Infect Dis 2008;46:787–794.CrossRefGoogle ScholarPubMed
26. Jenkins, TC, McCollister, BD, Sharma, R, et al. Epidemiology of healthcare-associated bloodstream infection caused by USA300 strains of methicillin-resistant Staphylococcus aureus in 3 affiliated hospitals. Infect Control Hosp Epidemiol 2009;30:233–241.CrossRefGoogle ScholarPubMed
27. Park, SH, Park, C, Yoo, JH, et al. Emergence of community-associated methicillin-resistant Staphylococcus aureus strains as a cause of healthcare-associated bloodstream infections in Korea. Infect Control Hosp Epidemiol 2009;30:146–155.Google Scholar
28. Carey, AJ, Delia-Latta, P, Huard, R, et al. Changes in the molecular epidemiological characteristics of methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit. Infect Control Hosp Epidemiol 2010;31:613–619.CrossRefGoogle Scholar
29. Milstone, AM, Carroll, KC, Ross, T, Shangraw, KA, Perl, TM. Community-associated methicillin-resistant Staphylococcus aureus strains in pediatric intensive care unit. Emerg Infect Dis 2010;16:647–655.Google Scholar
30. Lessa, FC, Mu, Y, Ray, SM, et al. Impact of USA300 methicillin-resistant Staphylococcus aureus on clinical outcomes of patients with pneumonia or central line-associated bloodstream infections. Clin Infect Dis 2012;55:232–241.Google Scholar
31. Bhalla, A, Pultz, NJ, Gries, DM, et al. Acquisition of nosocomial pathogens on hands after contact with environmental surfaces near hospitalized patients. Infect Control Hosp Epidemiol 2004; 25:164–167.Google Scholar
32. Smith, MA, Mathewson, JJ, Ulert, IA, Scerpella, EG, Ericsson, CD. Contaminated stethoscopes revisited. Arch Intern Med 1996; 156:82–84.Google Scholar
33. Cohen, HA, Liora, H, Paret, G. Aurioscope earpieces—a potential vector of infection? Int J Pediatr Otorhinolaryngol 1998;45: 47–50.Google Scholar
34. Bernard, L, Kereveur, A, Durand, D, et al. Bacterial contamination of hospital physicians’ stethoscopes. Infect Control Hosp Epidemiol 1999;20:626–628.CrossRefGoogle ScholarPubMed
35. Embil, JM, McLeod, JA, Al-Barrak, AM, et al. An outbreak of methicillin resistant Staphylococcus aureus on a burn unit: potential role of contaminated hydrotherapy equipment. Burns 2001;27:681–688.CrossRefGoogle ScholarPubMed
36. Breathnach, AS, Jenkins, DR, Pedler, SJ. Stethoscopes as possible vectors of infection by staphylococci. BMJ 1992;305:1573–1574.CrossRefGoogle ScholarPubMed
37. Boyce, JM, Potter-Bynoe, G, Chenevert, C, King, T. Environmental contamination due to methicillin-resistant Staphylococcus aureus: possible infection control implications. Infect Control Hosp Epidemiol 1997;18:622–627.CrossRefGoogle ScholarPubMed
38. Sexton, T, Clarke, P, O’Neill, E, Dillane, T, Humphreys, H. Environmental reservoirs of methicillin-resistant Staphylococcus aureus in isolation rooms: correlation with patient isolates and implications for hospital hygiene. J Hosp Infect 2006;62:187–194.CrossRefGoogle ScholarPubMed
39. Weber, DJ, Anderson, D, Rutala, WA. The role of the surface environment in healthcare-associated infections. Curr Opin Infect Dis 2013;26:338–344.CrossRefGoogle ScholarPubMed
40. Weber, DJ, Rutala, WA. Understanding and preventing transmission of healthcare-associated pathogens due to the contaminated hospital environment. Infect Control Hosp Epidemiol 2013;34:449–452.Google Scholar
41. Huang, SS, Datta, R, Platt, R. Risk of acquiring antibiotic-resistant bacteria from prior room occupants. Arch Intern Med 2006;166:1945–1951.Google Scholar
42. National Healthcare Safety Network. Multidrug-Resistant Organism and Clostridium difficile Infection (MDRO/CDI) Module. 2014. http://www.cdc.gov/nhsn/PDFs/pscManual/12pscMDRO_CDADcurrent.pdf. Accessed March 11, 2014.Google Scholar
43. National Healthcare Safety Network. CDC/NHSN Surveillance Definition of Healthcare-Associated Infection and Criteria for Specific Types of Infections in the Acute Care Setting. 2014. http://www.cdc.gov/nhsn/PDFs/pscManual/17pscNosInfDef_current.pdf. Accessed March 11, 2014.Google Scholar
44. Cohen, AL, Calfee, D, Fridkin, SK, et al. Recommendations for metrics for multidrug-resistant organisms in healthcare settings: SHEA/HICPAC position paper. Infect Control Hosp Epidemiol 2008;29:901–913.CrossRefGoogle ScholarPubMed
45. Siegel, JD, Rhinehart, E, Jackson, M, Chiarello, L. Management of multidrug-resistant organisms in health care settings, 2006. Am J Infect Control 2007;35:S165–S193.Google Scholar
46. Coia, JE, Duckworth, GJ, Edwards, DI, et al. Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities. } Hosp Infect 2006; 63(suppl 1):S1–S44.Google Scholar
47. Infection Prevention Working Party. MRSA in Hospitals. 2012. http://www.wip.n1/UK/free_content/Richtlijnen/l10530%20MRSA%20hospital%20def.pdf. Accessed June 4, 2013.Google Scholar
48. Dutch Working Party on Antibiotic Policy. Optimalization of the Antibiotic Policy in the Netherlands XI: Revision Swab Guideline for the Treatment of MRSA Carriage. 2012. http://www.swab.nl/swab/cms3.nsf/uploads/51DB72E670CAC33BC12579BF00342A95/$FILE/SwabrichtlijnMRSAherziening%2015022012_EN.pdf. Accessed June 4, 2013.Google Scholar
49. Institute for Healthcare Improvement. How to Guide: Reduce MRSA Infection. 2011.Google Scholar
50. Association for Professionals in Infection Control and Epidemiology. Guide to the Elimination of Methicillin-Resistant Staohylococcus aureus (MRSA) Transmission in Hospital Settings. 2nd ed. 2010.Google Scholar
51. Johnson, PD, Martin, R, Burrell, LJ, et al. Efficacy of an alcohol/chlorhexidine hand hygiene program in a hospital with high rates of nosocomial methicillin-resistant Staphylococcus aureus (MRSA) infection. Med J Aust 2005;183:509–514.Google Scholar
52. Gopal Rao, G, Jeanes, A, Osman, M, Aylott, C, Green, J. Marketing hand hygiene in hospitals—a case study, J Hosp Infect 2002; 50:42–47.Google Scholar
53. Morgan, DJ, Rogawski, E, Thorn, KA, et al. Transfer of multidrug-resistant bacteria to healthcare workers’ gloves and gowns after patient contact increases with environmental contamination. Crit Care Med 2012;40:1045–1051.CrossRefGoogle ScholarPubMed
54. Chang, S, Sethi, AK, Stiefel, U, Cadnum, JL, Donskey, CJ. Occurrence of skin and environmental contamination with methicillin-resistant Staphylococcus aureus before results of poly-merase chain reaction at hospital admission become available. Infect Control Hosp Epidemiol 2010;31:607–612.Google Scholar
55. Siegel, JD, Rhinehart, E, Jackson, M, Chiarello, L. 2007 Guideline for isolation precautions: preventing transmission of infectious agents in health care settings. Am J Infect Control. 2007;35:S65–S164.Google Scholar
56. Scanvic, A, Denic, L, Gaillon, S, Giry, P, Andremont, A, Lucet, JC. Duration of colonization by methicillin-resistant Staphylococcus aureus after hospital discharge and risk factors for prolonged carriage. Clin Infect Dis 2001;32:1393–1398.Google Scholar
57. Sanford, MD, Widmer, AR, Bale, MJ, Jones, RN, Wenzel, RP. Efficient detection and long-term persistence of the carriage of methicillin-resistant Staphylococcus aureus . Clin Infect Dis 1994; 19:1123–1128.CrossRefGoogle ScholarPubMed
58. Kirkland, KB, Weinstein, JM. Adverse effects of contact isolation. Lancet 1999;354:1177–1178.Google Scholar
59. Saint, S, Higgins, LA, Nallamothu, BK, Chenoweth, C. Do physicians examine patients in contact isolation less frequently? a brief report. Am J Infect Control 2003;31:354–356.Google Scholar
60. Evans, HL, Shaffer, MM, Hughes, MG, et al. Contact isolation in surgical patients: a barrier to care? Surgery 2003; 134:180–188.Google Scholar
61. Catalano, G, Houston, SH, Catalano, MC, et al. Anxiety and depression in hospitalized patients in resistant organism isolation. South Med J 2003;96:141–145.CrossRefGoogle ScholarPubMed
62. Day, HR, Perencevich, EN, Harris, AD, et al. Do contact precautions cause depression? a two-year study at a tertiary care medical centre. J Hosp Infect 2011;79:103–107.Google Scholar
63. Stelfox, HT, Bates, DW, Redelmeier, DA. Safety of patients isolated for infection control. JAMA 2003;290:1899–1905.Google Scholar
64. Harris, AD, Pineles, L, Belton, B, et al. Universal glove and gown use and acquisition of antibiotic-resistant bacteria in the ICU: a randomized trial. JAMA 2013;310:1571–1580.Google ScholarPubMed
65. Diekema, DJ, Edmond, MB. Look before you leap: active surveillance for multidrug-resistant organisms. Clin Infect Dis 2007;44:1101–1107.Google Scholar
66. Hardy, KJ, Oppenheim, BA, Gossain, S, Gao, F, Hawkey, PM. A study of the relationship between environmental contamination with methicillin-resistant Staphylococcus aureus (MRSA) and patients’ acquisition of MRSA. Infect Control Hosp Epidemiol 2006;27:127–132.Google Scholar
67. French, GL, Otter, JA, Shannon, KP, Adams, NM, Watling, D, Parks, MJ. Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus (MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapour decontamination. J Hosp Infect 2004;57:31–37.Google Scholar
68. Oie, S, Hosokawa, I, Kamiya, A. Contamination of room door handles by methicillin-sensitive/methicillin-resistant Staphylococcus aureus . J Hosp Infect 2002;51:140–143.CrossRefGoogle ScholarPubMed
69. Rampling, A, Wiseman, S, Davis, L, et al. Evidence that hospital hygiene is important in the control of methicillin-resistant Staphylococcus aureus . J Hosp Infect 2001;49:109–116.Google Scholar
70. Vajravelu, RK, Guerrero, DM, Jury, LA, Donskey, CJ. Evaluation of stethoscopes as vectors of Clostridium difficile and methicillin-resistant Staphylococcus aureus . Infect Control Hosp Epidemiol 2012;33:96–98.CrossRefGoogle ScholarPubMed
71. Carling, P. Methods for assessing the adequacy of practice and improving room disinfection. Am J Infect Control 2013;41:S20–S25.Google Scholar
72. Dancer, SJ. Importance of the environment in meticillin-resistant Staphylococcus aureus acquisition: the case for hospital cleaning. Lancet Infect Dis 2008;8:101–113.Google Scholar
73. Carling, PC, Parry, MF, Bruno-Murtha, LA, Dick, B. Improving environmental hygiene in 27 intensive care units to decrease multidrug-resistant bacterial transmission. Crit Care Med 2010; 38:1054–1059.Google Scholar
74. Abad, C, Fearday, A, Safdar, N. Adverse effects of isolation in hospitalised patients: a systematic review. J Hosp Infect 2010; 76:97–102.Google Scholar
75. Salgado, CD, Farr, BM. What proportion of hospital patients colonized with methicillin-resistant Staphylococcus aureus are identified by clinical microbiological cultures? Infect Control Hosp Epidemiol 2006;27:116–121.Google Scholar
76. Huang, SS, Rifas-Shiman, SL, Warren, DK, et al. Improving methicillin-resistant Staphylococcus aureus surveillance and reporting in intensive care units. J Infect Dis 2007;195:330–338.Google Scholar
77. Jain, R, Krsalovic, SM, Evans, ME, et al. Veterans Affairs initiative to prevent methicillin-resistant Staphylococcus aureus infections. N Engl J Med 2011;364:1419–1430.Google Scholar
78. West, TE, Guerry, C, Hiott, M, Morrow, N, Ward, K, Salgado, CD. Effect of targeted surveillance for control of methicillin-resistant Staphylococcus aureus in a community hospital system. Infect Control Hosp Epidemiol 2006;27:233–238.Google Scholar
79. Huang, SS, Yokoe, DS, Hinrichsen, VL, et al. Impact of routine intensive care unit surveillance cultures and resultant barrier precautions on hospital-wide methicillin-resistant Staphylococcus aureus bacteremia. Clin Infect Dis 2006;43:971–978.Google Scholar
80. Lucet, JC, Paoletti, X, Lolom, I, et al. Successful long-term program for controlling methicillin-resistant Staphylococcus aureus in intensive care units. Intensive Care Med 2005;31:1051–1057.Google Scholar
81. Marshall, C, Richards, M, McBryde, E. Do active surveillance and contact precautions reduce MRSA acquisition? a prospective interrupted time series. PLoS ONE 2013;8:e58112.Google Scholar
82. Perlin, JB, Hickok, JD, Septimus, EJ, Moody, JA, Englebright, JD, Bracken, RM. A bundled approach to reduce methicillin-resistant Staphylococcus aureus infections in a system of community hospitals. J Healthc Qual 2013;35:57–68.CrossRefGoogle Scholar
83. Harbarth, S, Fankhauser, C, Schrenzel, J, et al. Universal screening for methicillin-resistant Staphylococcus aureus at hospital admission and nosocomial infection in surgical patients. JAMA 2008;299:1149–1157.Google Scholar
84. Huskins, WC, Huckabee, CM, O’Grady, NP, et al. Intervention to reduce transmission of resistant bacteria in intensive care. N Engl J Med 2011;364:1407–1418.CrossRefGoogle ScholarPubMed
85. Agency for Healthcare Quality Research. Screening for Methi-cillin-Resistant Staphylococcus aureus (MRSA). 2013. http://www.effectivehealthcare.ahrq.gov/ehc/products/228/1550/MRSA-screening-report-130617.pdf. Accessed July 18, 2013.Google Scholar
86. Gurieva, TV, Bootsma, MC, Bonten, MJ. Decolonization of patients and health care workers to control nosocomial spread of methicillin-resistant Staphylococcus aureus: a simulation study. BMC Infect Dis 2012;12:302.Google Scholar
87. Miller, MA, Dascal, A, Portnoy, J, Mendelson, J. Development of mupirocin resistance among methicillin-resistant Staphylococcus aureus after widespread use of nasal mupirocin ointment. Infect Control Hosp Epidemiol 1996;17:811–813.Google Scholar
88. Lepainteur, M, Royer, G, Bourrel, AS, et al. Prevalence of resistance to antiseptics and mupirocin among invasive coagulase-negative staphylococci from very preterm neonates in NICU: the creeping threat? J Hosp Infect 2013;83:333–336.CrossRefGoogle ScholarPubMed
89. Teo, BW, Low, SJ, Ding, Y, Koh, TH, Hsu, LY. High prevalence of mupirocin-resistant staphylococci in a dialysis unit where mupirocin and chlorhexidine are routinely used for prevention of catheter-related infections. J Med Microbiol 2011;60:865–867.Google Scholar
90. Ridenour, G, Lampen, R, Federspiel, J, Kritchevsky, S, Wong, E, Climo, M. Selective use of intranasal mupirocin and chlorhexidine bathing and the incidence of methicillin-resistant Staphylococcus aureus colonization and infection among intensive care unit patients. Infect Control Hosp Epidemiol 2007;28:1155–1161.CrossRefGoogle ScholarPubMed
91. Saiman, L, Cronquist, A, Wu, F, et al. An outbreak of methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit. Infect Control Hosp Epidemiol 2003;24:317–321.CrossRefGoogle Scholar
92. Nambiar, S, Herwaldt, LA, Singh, N. Outbreak of invasive disease caused by methicillin-resistant Staphylococcus aureus in neonates and prevalence in the neonatal intensive care unit. Pediatr Crit Care Med 2003;4:220–226.Google Scholar
93. Hitomi, S, Kubota, M, Mori, N, et al. Control of a methicillin-resistant Staphylococcus aureus outbreak in a neonatal intensive care unit by unselective use of nasal mupirocin ointment. J Hosp Infect 2000;46:123–129.CrossRefGoogle Scholar
94. Herwaldt, LA. Reduction of Staphylococcus aureus nasal carriage and infection in dialysis patients. J Hosp Infect 1998;40(suppl B):S13–S23.CrossRefGoogle ScholarPubMed
95. Miller, LG, Eells, SJ, Taylor, AR, et al. Staphylococcus aureus colonization among household contacts of patients with skin infections: risk factors, strain discordance, and complex ecology. Clin Infect Dis 2012;54:1523–1535.Google Scholar
96. Kluytmans, JA, Mouton, JW, VandenBergh, MF, et al. Reduction of surgical-site infections in cardiothoracic surgery by elimination of nasal carriage of Staphylococcus aureus . Infect Control Hosp Epidemiol 1996;17:780–785.Google Scholar
97. Pofahl, WE, Goettler, CE, Ramsey, KM, Cochran, MK, Nobles, DL, Rotondo, ME Active surveillance screening of MRSA and eradication of the carrier state decreases surgical-site infections caused by MRSA. J Am Coll Surg 2009;208:981–986.Google Scholar
98. Wilcox, MH, Hall, J, Pike, H, et al. Use of perioperative mupirocin to prevent methicillin-resistant Staphylococcus aureus (MRSA) orthopaedic surgical site infections. J Hosp Infect 2003; 54:196–201.Google Scholar
99. Schweizer, M, Perencevich, E, McDanel, J, et al. Effectiveness of a bundled intervention of decolonization and prophylaxis to decrease gram positive surgical site infections after cardiac or orthopedic surgery: systematic review and meta-analysis. BMJ 2013;346:f2743.Google Scholar
100. Anderson, DJ, Podgorny, K, Berríos-Torres, SI, et al. Strategies to prevent surgical site infections in acute care hospitals: 2014 update. Infect Control Hosp Epidemiol 2014;35(6):605–627.CrossRefGoogle ScholarPubMed
101. Vernon, MO, Hayden, MK, Trick, WE, Hayes, RA, Blom, DW, Weinstein, RA. Chlorhexidine gluconate to cleanse patients in a medical intensive care unit: the effectiveness of source control to reduce the bioburden of vancomycin-resistant enterococci. Arch Intern Med 2006;166:306–312.CrossRefGoogle Scholar
102. Munoz-Price, LS, Hota, B, Sterner, A, Weinstein, RA. Prevention of bloodstream infections by use of daily chlorhexidine baths for patients at a long-term acute care hospital. Infect Control Hosp Epidemiol 2009;30:1031–1035.Google Scholar
103. Evans, HL, Dellit, TH, Chan, J, Nathens, AB, Maier, RV, Cuschieri, J. Effect of chlorhexidine whole-body bathing on hospital-acquired infections among trauma patients. Arch Surg 2010; 145:240–246.Google Scholar
104. Popovich, KJ, Hota, B, Hayes, R, Weinstein, RA, Hayden, MK. Effectiveness of routine patient cleansing with chlorhexidine gluconate for infection prevention in the medical intensive care unit. Infect Control Hosp Epidemiol 2009;30:959–963.Google Scholar
105. Climo, MW, Yokoe, DS, Warren, DK, et al. Effect of daily chlorhexidine bathing on hospital-acquired infection. N Engl J Med 2013;368:533–542.Google Scholar
106. Huang, SS, Septimus, E, Kleinman, K, et al. Targeted versus universal decolonization to prevent ICU infection. N Engl J Med 2013;368:2255–2265.Google Scholar
107. Milstone, AM, Elward, A, Song, X, et al. Daily chlorhexidine bathing to reduce bacteraemia in critically ill children: a mul-ticentre, cluster-randomised, crossover trial. Lancet 2013;381: 1099–1106.CrossRefGoogle ScholarPubMed
108. Climo, MW, Sepkowitz, KA, Zuccotti, G, et al. The effect of daily bathing with chlorhexidine on the acquisition of methicillin-resistant Staphylococcus aureus, vancomycin-resistant Entero-coccus, and healthcare-associated bloodstream infections: results of a quasi-experimental multicenter trial. Crit Care Med 2009;37:1858–1865.CrossRefGoogle Scholar
109. Marschall, J, Mermel, LA, Fakih, M, et al. Strategies to prevent central line-associated bloodstream infections in acute care hospitals: 2014 update. Infect Control Hosp Epidemiol 2014; 35(7):753–771 (in this issue).Google Scholar
110. Karki, S, Cheng, AC. Impact of non-rinse skin cleansing with chlorhexidine gluconate on prevention of healthcare-associated infections and colonization with multi-resistant organisms: a systematic review. J Hosp Infect 2012;82:71–84.Google Scholar
111. Derde, LP, Cooper, BS, Goossens, H, et al. Interventions to reduce colonisation and transmission of antimicrobial-resistant bacteria in intensive care units: an interrupted time series study and cluster randomised trial. Lancet Infect Dis 2013;14(1):31–9.Google Scholar
112. Rupp, ME, Cavalieri, RJ, Lyden, E, et al. Effect of hospital-wide chlorhexidine patient bathing on healthcare-associated infections. Infect Control Hosp Epidemiol 2012;33:1094–1100.Google Scholar
113. Delaney, HM, Wang, E, Melish, M. Comprehensive strategy including prophylactic mupirocin to reduce Staphylococcus aureus colonization and infection in high-risk neonates. J Perinatol 2013;33:313–318.Google Scholar
114. Fritz, SA, Hogan, PG, Hayek, G, et al. Staphylococcus aureus colonization in children with community-associated Staphylococcus aureus skin infections and their household contacts. Arch Pediatr Adolesc Med 2012;166:551–557.CrossRefGoogle ScholarPubMed
115. Clinical and Laboratory Standards Institute (CLSI). Performance Standards f or Antimicrobial Susceptibility Testing: Twenty-Third Informational Supplement. Wayne, PA: CLSI, 2013. CLSI document M100-S23.Google Scholar
116. Center for Medicare & Medicaid Services. Federal Register. 2012. http://www.gpo.gov/fdsys/pkg/FR-2012-05-ll/pdf/2012-9985.pdf. Accessed July 18, 2013.Google Scholar
117. The Joint Commission. National Patient Safety Goals. 2013. http://www.jointcommission.org/standards_information/npsgs.aspx. Accessed July 18, 2013.Google Scholar
118. Center for Medicare & Medicaid Services. State Operations Manual: Appendix A—Survey Protocol, Regulations and Interpretive Guidelines for Hospitals. Rev. 84, 06-07-13. 2013. http://www.cms.gov/Regulations-and-Guidance/Guidance/Manuals/downloads/soml07ap_a_hospitals.pdf. Accessed July 18, 2013.Google Scholar
119. Ellingson, K, Hass, JP, Aiello, AE, et al. Strategies to prevent healthcare-associated infections through hand hygiene. Infect Control Hosp Epidemiol 2014 (forthcoming).Google Scholar
120. Seto, WH. Training the work force—models for effective education in infection control. J Hosp Infect 1995;30(suppl):241–247.Google Scholar
121. Sehulster, L, Chinn, RY. Guidelines for environmental infection control in health-care facilities: recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC). MMWR Recomm Rep 2003;52:1–42.Google ScholarPubMed
122. Rutala, WA, Weber, DJ; Healthcare Infection Control Practices Advisory Committee (HICPAC). Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008. http://www.cdc.gov/hicpac/pdf/guidelines/Disinfection_Nov_2008.pdf. Accessed July 18, 2013.Google Scholar
123. Otter, JA, Yezli, S, Perl, TM, Barbut, F, French, GL. The role of “no-touch” automated room disinfection systems in infection prevention and control. J Hosp Infect 2013;83:1–13.Google Scholar
124. Passaretti, CL, Otter, JA, Reich, NG, et al. An evaluation of environmental decontamination with hydrogen peroxide vapor for reducing the risk of patient acquisition of multidrug-resistant organisms. Clin Infect Dis 2013;56:27–35.CrossRefGoogle ScholarPubMed
125. Salgado, CD, Sepkowitz, KA, John, JF, et al. Copper surfaces reduce the rate of healthcare-acquired infections in the intensive care unit. Infect Control Hosp Epidemiol 2013;34:479–486.CrossRefGoogle ScholarPubMed
126. Kac, G, Grohs, P, Durieux, P, et al. Impact of electronic alerts on isolation precautions for patients with multidrug-resistant bacteria. Arch Intern Med 2007;167:2086–2090.Google Scholar
127. Halpin, H, Shortell, SM, Milstein, A, Vanneman, M. Hospital adoption of automated surveillance technology and the implementation of infection prevention and control programs. Am J Infect Control 2011;39:270–276.Google Scholar
128. Wright, MO. Automated surveillance and infection control: toward a better tomorrow. Am J Infect Control 2008;36:S1–S6.Google Scholar
129. Mozzillo, KL, Ortiz, N, Miller, LG. Patients with methicillin-resistant Staphylococcus aureus infection: twenty-first century lepers. J Hosp Infect 2010;75:132–134.Google Scholar
130. Briggs, JJ, Milstone, AM. Changes over time in caregivers’ knowledge, attitudes, and behaviors regarding methicillin-resistant Staphylococcus aureus . J Pediatr 2011;158:1039.Google Scholar
131. Wang, JT, Chang, SC, Ko, WJ, et al. A hospital-acquired outbreak of methicillin-resistant Staphylococcus aureus infection initiated by a surgeon carrier, J Hosp Infect 2001;47:104–109.Google Scholar
132. Faibis, F, Laporte, C, Fiacre, A, et al. An outbreak of methicillin-resistant Staphylococcus aureus surgical-site infections initiated by a healthcare worker with chronic sinusitis. Infect Control Hosp Epidemiol 2005;26:213–215.Google Scholar
133. Coombs, GW, Van Gessel, H, Pearson, JC, Godsell, MR, O’Brien, FG, Christiansen, KJ. Controlling a multicenter outbreak involving the New York/Japan methicillin-resistant Staphylococcus aureus clone. Infect Control Hosp Epidemiol 2007;28:845–852.Google Scholar
134. Pittet, D, Allegranzi, B, Sax, H, Dharan, S, Pessoa-Silva, CL, Donaldson, L, Boyce, JM. Evidence-based model for hand transmission during patient care and the role of improved practices. Lancet Infect Dis 2006;6:641–652.Google Scholar
135. Thompson, RL, Cabezudo, I, Wenzel, RP. Epidemiology of nosocomial infections caused by methicillin-resistant Staphylococcus aureus . Ann Intern Med 1982;97:309–317.Google Scholar
136. Cox, RA, Conquest, C. Strategies for the management of healthcare staff colonized with epidemic methicillin-resistant Staphylococcus aureus . J Hosp Infect 1997;35:117–127.Google Scholar
137. Albrich, WC, Harbarth, S. Health-care workers: source, vector, or victim of MRSA? Lancet Infect Dis 2008;8:289–301.Google Scholar
138. Cookson, B, Peters, B, Webster, M, Phillips, I, Rahman, M, Noble, W. Staff carriage of epidemic methicillin-resistant Staphylococcus aureus . J Clin Microbiol 1989;27:1471–1476.Google Scholar
139. Bertin, ML, Vinski, J, Schmitt, S, et al. Outbreak of methicillin-resistant Staphylococcus aureus colonization and infection in a neonatal intensive care unit epidemiologically linked to a healthcare worker with chronic otitis. Infect Control Hosp Epidemiol 2006;27:581–585.CrossRefGoogle Scholar
140. Stein, M, Navon-Venezia, S, Chmelnitsky, I, et al. An outbreak of new, nonmultidrug-resistant, methicillin-resistant Staphylococcus aureus strain (SCCmec type IILA variant-1 ) in the neonatal intensive care unit transmitted by a staff member. Pediatr Infect Dis J 2006;25:557–559.Google Scholar
141. Meier, PA, Carter, CD, Wallace, SE, Hollis, RJ, Pfaller, MA, Herwaldt, LA. A prolonged outbreak of methicillin-resistant Staphylococcus aureus in the burn unit of a tertiary medical center. Infect Control Hosp Epidemiol 1996;17:798–802.Google Scholar
142. Blok, HE, Troelstra, A, Kamp-Hopmans, TE, et al. Role of healthcare workers in outbreaks of methicillin-resistant Staphylococcus aureus: a 10-year evaluation from a Dutch university hospital. Infect Control Hosp Epidemiol 2003;24:679–685.Google Scholar
143. Wendt, C, Schinke, S, Wurttemberger, M, Oberdorfer, K, Hensley, O, von Baum, H. Value of whole-body washing with chlorhexidine for the eradication of methicillin-resistant Staphylococcus aureus: a randomized, placebo-controlled, double-blind clinical trial. Infect Control Hosp Epidemiol 2007;28:1036–1043.Google Scholar
144. Popovich, KJ, Lyles, R, Hayes, R, et al. Relationship between chlorhexidine gluconate skin concentration and microbial density on the skin of critically ill patients bathed daily with chlorhexidine gluconate. Infect Control Hosp Epidemiol 2012;33:889–896.Google Scholar
145. Tamma, PD, Aucott, SW, Milstone, AM. Chlorhexidine use in the neonatal intensive care unit: results from a national survey. Infect Control Hosp Epidemiol 2010;31:846–849.Google Scholar
146. Chapman, AK, Aucott, SW, Milstone, AM. Safety of chlorhexidine gluconate used for skin antisepsis in the preterm infant. J Perinatol 2012;32:4–9.Google Scholar
147. Sitzlar, B, Deshpande, A, Fertelli, D, Kundrapu, S, Sethi, AK, Donskey, CJ. An environmental disinfection Odyssey: evaluation of sequential interventions to improve disinfection of Clostridium difficile isolation rooms. Infect Control Hosp Epidemiol 2013;34:459–465.Google Scholar
148. Carling, PC, Parry, MF, Von Beheren, SM. Identifying opportunities to enhance environmental cleaning in 23 acute care hospitals. Infect Control Hosp Epidemiol 2008;29:1–7.Google Scholar
149. Morgan, DJ, Day, HR, Furano, JP, et al. Improving efficiency in active surveillance for methicillin-resistant Staphylococcus aureus or vancomycin-resistant Enterococcus at hospital admission. Infect Control Hosp Epidemiol 2010;31:1230–1235.Google Scholar
150. Reilly, JS, Stewart, S, Christie, P, et al. Universal screening for meticillin-resistant Staphylococcus aureus in acute care: risk factors and outcome from a multicentre study. J Hosp Infect 2012; 80:31–35.Google Scholar
151. Haley, CC, Mittal, D, Laviolette, A, Jannapureddy, S, Parvez, N, Haley, RW. Methicillin-resistant Staphylococcus aureus infection or colonization present at hospital admission: multivariable risk factor screening to increase efficiency of surveillance culturing. J Clin Microbiol 2007;45:3031–3038.Google Scholar
152. Riedel, S, Von Stein, D, Richardson, K, et al. Development of a prediction rule for methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus carriage in a Veterans Affairs medical center population. Infect Control Hosp Epidemiol 2008;29:969–971.Google Scholar
153. Harbarth, S, Sax, H, Fankhauser-Rodriguez, C, Schrenzel, J, Agostinho, A, Pittet, D. Evaluating the probability of previously unknown carriage of MRSA at hospital admission. Am J Med 2006; 119:275.e215-275.e223.Google Scholar
154. Lautenbach, E, Nachamkin, I, Hu, B, et al. Surveillance cultures for detection of methicillin-resistant Staphylococcus aureus: diagnostic yield of anatomic sites and comparison of provider-and patient-collected samples. Infect Control Hosp Epidemiol 2009;30:380–382.Google Scholar
155. Furuno, JP, Harris, AD, Wright, MO, et al. Value of performing active surveillance cultures on intensive care unit discharge for detection of methicillin-resistant Staphylococcus aureus . Infect Control Hosp Epidemiol 2007;28:666–670.CrossRefGoogle ScholarPubMed
156. Senn, L, Basset, P, Nahimana, I, Zanetti, G, Blanc, DS. Which anatomical sites should be sampled for screening of methicillin-resistant Staphylococcus aureus carriage by culture or by rapid PCR test? Clin Micro Infect 2012;18:E31-E33.Google Scholar
157. McKinnell, JA, Huang, SS, Eells, SJ, Cui, E, Miller, LG. Quantifying the impact of extranasal testing of body sites for methicillin-resistant Staphylococcus aureus colonization at the time of hospital or intensive care unit admission. Infect Control Hosp Epidemiol 2013;34:161–170.Google Scholar
158. Shurland, SM, Stine, OC, Venezia, RA, et al. Colonization sites of USA300 methicillin-resistant Staphylococcus aureus in residents of extended care facilities. Infect Control Hosp Epidemiol 2009;30:313–318.Google Scholar
159. Matheson, A, Christie, P, Stari, T, et al. Nasal swab screening for methicillin-resistant Staphylococcus aureus—how well does it perform? a cross-sectional study. Infect Control Hosp Epidemiol 2012;33:803–808.Google Scholar
160. Eveillard, M, de Lassence, A, Lancien, E, Barnaud, G, Ricard, JD, Joly-Guillou, ML. Evaluation of a strategy of screening multiple anatomical sites for methicillin-resistant Staphylococcus aureus at admission to a teaching hospital. Infect Control Hosp Epidemiol 2006;27:181–184.Google Scholar
161. Popovich, KJ, Hota, B, Aroutcheva, A, et al. Community-associated methicillin-resistant Staphylococcus aureus colonization burden in HIV-infected patients. Clin Infect Dis 2013;56:1067–1074.Google Scholar
162. Gerber, SI, Jones, RC, Scott, MV, et al. Management of outbreaks of methicillin-resistant Staphylococcus aureus infection in the neonatal intensive care unit: a consensus statement. Infect Control Hosp Epidemiol 2006;27:139–145.Google Scholar
163. Rosenthal, A, White, D, Churilla, S, Brodie, S, Katz, KC. Optimal surveillance culture sites for detection of methicillin-resistant Staphylococcus aureus in newborns. J Clin Microbiol 2006;44: 4234–4236.Google Scholar
164. Bishop, EJ, Grabsch, EA, Ballard, SA, et al. Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual-specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus . J Clin Microbiol 2006;44:2904–2908.Google Scholar
165. Diederen, B, van Duijn, I, van Belkum, A, Willemse, P, van Keulen, P, Kluytmans, J. Performance of CHROMagar MRSA medium for detection of methicillin-resistant Staphylococcus aureus . J Clin Microbiol 2005;43:1925–1927.Google Scholar
166. Diederen, BM, van Leest, ML, van Duijn, I, Willemse, P, van Keulen, PH, Kluytmans, JA. Performance of MRSA ID, a new chromogenie medium for detection of methicillin-resistant Staphylococcus aureus . J Clin Microbiol 2006;44:586–588.Google Scholar
167. Flayhart, D, Hindler, JF, Bruckner, DA, et al. Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. J Clin Microbiol 2005;43:5536–5540.Google Scholar
168. Stoakes, L, Reyes, R, Daniel, J, et al. Prospective comparison of a new chromogenie medium, MRSAselect, to CHROMagar MRSA and mannitol-salt medium supplemented with oxacillin or cefoxitin for detection of methicillin-resistant Staphylococcus aureus . J Clin Microbiol 2006;44:637–639.Google Scholar
169. Han, Z, Lautenbach, E, Fishman, N, Nachamkin, I. Evaluation of mannitol salt agar, CHROMagar Staph aureus and CHROMagar MRSA for detection of meticillin-resistant Staphylococcus aureus from nasal swab specimens. J Clin Microbiol 2007;56:43–46.Google Scholar
170. Wassenberg, MW, Kluytmans, JA, Box, AT, et al. Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenie agar: a prospective study to evaluate costs and effects. Clin Micro Infect 2010;16:1754–1761.Google Scholar
171. Denys, GA, Renzi, PB, Koch, KM, Wissel, CM. Three-way comparison of BBL CHROMagar MRSAII, MRSA select, and MR Spectra SA for detection of methicillin-resistant Staphylococcus aureus isolates in nasal surveillance cultures. J Clin Microbiol 2013;51:202–205.Google Scholar
172. Safdar, N, Narans, L, Gordon, B, Maki, DG. Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods. J Clin Microbiol 2003;41:3163–3166.Google Scholar
173. Wolk, DM, Marx, JL, Dominguez, L, Driscoll, D, Schifman, RB. Comparison of MRSASelect agar, CHROMagar methicillin-resistant Staphylococcus aureus (MRSA) medium, and Xpert MRSA PCR for detection of MRSA in nares: diagnostic accuracy for surveillance samples with various bacterial densities. J Clin Microbiol 2009;47:3933–3936.Google Scholar
174. Nonhoff, C, Denis, O, Brenner, A, et al. Comparison of three chromogenie media and enrichment broth media for the detection of methicillin-resistant Staphylococcus aureus from mucocutaneous screening specimens: comparison of MRSA chromogenie media. Eur J Clin Microbiol Infect Dis 2009;28:363–369.Google Scholar
175. Huletsky, A, Lebel, P, Picard, FJ, et al. Identification of methicillin-resistant Staphylococcus aureus carriage in less than 1 hour during a hospital surveillance program. Clin Infect Dis 2005; 40:976–981.Google Scholar
176. Warren, DK, Liao, RS, Merz, LR, Eveland, M, Dunne, WM Jr. Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J Clin Miaobiol 2004;42:5578–5581.Google Scholar
177. Drews, SJ, Willey, BM, Kreiswirth, N, et al. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting, J Clin Microbiol 2006;44:3794–3796.Google Scholar
178. Wong, H, Louie, L, Lo, RY, Simor, AE. Characterization of Staphylococcus aureus isolates with a partial or complete absence of staphylococcal cassette chromosome elements. J Clin Microbiol 2010;48:3525–3531.Google Scholar
179. Blanc, DS, Basset, P, Nahimana-Tessemo, I, Jaton, K, Greub, G, Zanetti, G. High proportion of wrongly identified methicillin-resistant Staphylococcus aureus carriers by use of a rapid commercial PCR assay due to presence of staphylococcal cassette chromosome element lacking the mecA gene, J Clin Microbiol 2011;49:722–724.Google Scholar
180. Bartels, MD, Boye, K, Rohde, SM, et al. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. J Clin Miaobiol 2009;47:1524–1527.Google Scholar
181. Laurent, C, Bogaerts, P, Schoevaerdts, D, et al. Evaluation of the Xpert MRSA assay for rapid detection of methicillin-resistant Staphylococcus aureus from nares swabs of geriatric hospitalized patients and failure to detect a specific SCC mec type IV variant. Eur J Clin Microbiol Infect Dis 2010;29:995–1002.Google Scholar
182. Cunningham, R, Jenks, P, Northwood, J, Wallis, M, Ferguson, S, Hunt, S. Effect on MRSA transmission of rapid PCR testing of patients admitted to critical care. J Hosp Infect 2007;65: 24–28.Google Scholar
183. Bootsma, MC, Diekmann, O, Bonten, MJ. Controlling methi-cillin-resistant Staphylococcus aureus: quantifying the effects of interventions and rapid diagnostic testing. Proc Nati Acad Sci USA 2006;103:5620–5625.Google Scholar
184. Olchanski, N, Mathews, C, Fusfeld, L, Jarvis, W. Assessment of the influence of test characteristics on the clinical and cost impacts of methicillin-resistant Staphylococcus aureus screening programs in US hospitals. Infect Control Hosp Epidemiol 2011; 32:250–257.Google Scholar
185. Li, J, Ulvin, K, Biboh, H, Kristiansen, IS. Cost-effectiveness of supplementing a broth-enriched culture test with the Xpert meticillin-resistant Staphylococcus aureus (MRSA) assay for screening inpatients at high risk of MRSA. J Hosp Infect 2012; 82:227–233.Google Scholar
186. Jeyaratnam, D, Whitty, CJ, Phillips, K, et al. Impact of rapid screening tests on acquisition of meticillin resistant Staphylococcus aureus: cluster randomised crossover trial. BMJ 2008; 336:927–930.Google Scholar
187. Safdar, N, Marx, J, Meyer, NA, Maki, DG. Effectiveness of preemptive barrier precautions in controlling nosocomial colonization and infection by methicillin-resistant Staphylococcus aureus in a burn unit. Am J Infect Control 2006;34:476–483.Google Scholar
188. Guyatt, GH, Oxman, AD, Vist, GE, et al. GRADE: an emerging consensus on rating quality of evidence and strength of recommendations. BMJ 2008;336(7650):924–926.Google Scholar
189. GRADE. Canadian Task Force on Preventive Health Care website. http://canadiantaskforce.ca/methods/grade/. Accessed December 31, 2013.Google Scholar