Hostname: page-component-76c49bb84f-6sxsx Total loading time: 0 Render date: 2025-07-09T21:51:21.519Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of Nested Polymerase Chain Reaction Based on Sequence-Based Typing of Clinical Samples to Determine the Source of Infection for Hospital-Acquired Legionnaires' Disease

Published online by Cambridge University Press:  02 January 2015

Maria Scaturro ,
Stefano Fontana  and
Maria Luisa Ricci
Maria Scaturro*
Affiliation:
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Stefano Fontana
Affiliation:
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Maria Luisa Ricci
Affiliation:
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
*
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy (e-mail: maria.scaturro@iss.it)
Get access Rights & Permissions [Opens in a new window]

Extract

The source of infection of a hospital-acquired Legionnaires' disease case was determined for the first time by nested polymerase chain reaction based on sequence-based typing. The typing was performed directly on DNA extracted from tissue samples, allowing a rapid epidemiological correlation with environmental isolates.

Information

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 32 , Issue 5 , May 2011 , pp. 510 - 512
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

1. Carratalà, J, Garcia-Vidal, C. An update on Legionella . Curr Opin Infect Dis 2010;23:152–157.Google Scholar
2. Diederen, BM, Bruin, JP, Scopes, E, Peeters, MF, IJzerman, EP. Evaluation of the Oxoid Xpect Legionella test kit for detection of Legionella pneumophila serogroup 1 antigen in urine. J Clin Microbiol 2009;47:2272–2274.CrossRefGoogle ScholarPubMed
3. Loens, K, Beck, T, Ursi, D, et al. Evaluation of different nucleic acid amplification techniques for the detection of M. pneumoniae, C. pneumoniae and Legionella spp. in respiratory specimens from patients with community-acquired pneumonia. J Microbiol Methods 2008;73:257–262.Google Scholar
4. Gaia, V, Fry, NK, Afshar, B, et al. Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila . J Clin Microbiol 2005;43:2047–2052.Google Scholar
5. Ratzow, S, Gaia, V, Helbig, JH, Fry, NK, Lück, PC. Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila sero-group 1 strains. J Clin Microbiol 2007;45:1965–1968.Google Scholar
6. Ginevra, C, Lopez, M, Forey, F, et al. Evaluation of a nested-PCR-derived sequence-based typing method applied directly to respiratory samples from patients with Legionnaires’ disease. J Clin Microbiol 2009;47:981–987.CrossRefGoogle ScholarPubMed
7. Helbig, JH, Bernander, S, Castellani Pastoris, M, et al. Pan-European study on culture-proven Legionnaires’ disease: distribution of Legionella pneumophila serogroups and monoclonal subgroups. Eur J Clin Microbiol Infect Dis 2002;21:710–716.Google Scholar
8. European Working Group for Legionella Infections, http://www.ewgli.org.Google Scholar
9. Fry, NK, Bangsborg, JM, Bergmans, A, et al. Designation of the European Working Group on Legionella Infection (EWGLI) amplified fragment length polymorphism types of Legionella pneumophila serogroup 1 and results of intercentre proficiency testing using a standard protocol. Eur J Clin Microbiol Infect Dis 2002;21:722–728.Google Scholar
10. Lück, PC, Ecker, C, Reischl, U, Linde, HJ, Stempka, R. Culture-independent identification of the source of an infection by direct amplification and sequencing of Legionella pneumophila DNA from a clinical specimen. J Clin Microbiol 2007;45:3143–3144.Google Scholar
11. Coscollá, M, González-Candelas, F. Direct sequencing of Legionella pneumophila from respiratory samples for sequence-based typing analysis. J Clin Microbiol 2009;47:2901–2905.Google Scholar