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Calbindin-D28k immunoreactivity in the mice thoracic spinal cord after space flight

Published online by Cambridge University Press:  22 May 2015

Valentina V. Porseva ,
Valentin V. Shilkin ,
Igor B. Krasnov  and
Petr M. Masliukov
Valentina V. Porseva
Affiliation:
Department of Human Anatomy, Yaroslavl State Medical University, Yaroslavl, Russia
Valentin V. Shilkin
Affiliation:
Department of Human Anatomy, Yaroslavl State Medical University, Yaroslavl, Russia
Igor B. Krasnov
Affiliation:
Laboratory of Gravitational Biology, Institute of Biomedical Problems of Russian Academy of Sciences, Moscow, Russia
Petr M. Masliukov*
Affiliation:
Department of Normal Physiology, Yaroslavl State Medical University, Yaroslavl, Russia
*
e-mail: mpm@yma.ac.ru
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Abstract

The aim of the work was to analyse changes in the location and morphological characteristics of calbindin (CB)-immunoreactive (IR) neurons of the thoracic spinal cord of C57BL/6N male mice after completion of a 30-day space flight on board the BION-M1 biosatellite (Russia, 2013). Space flight induced multidirectional changes of the number and morphological parameters of CB-positive neurons. The number of IR neurons increased in laminae I (from 10 to 17 neurons per section), II (from 42 to 67 cells per section) and IX (from two neurons per segment to two neurons per section), but CB disappeared in neurons of lamina VIII. Weightlessness did not affect the number of CB-IR neurons in laminae III–V and VII, including preganglionic sympathetic neurons. The cross-sectional area of CB-IR neurons decreased in lamina II and VII (group of partition cells) and increased in laminae III–V and IX. After a space flight, few very large neurons with long dendrites appeared in lamina IV. The results obtained give evidence about substantial changes in the calcium buffer system and imbalance of different groups of CB-IR neurons due to reduction of afferent information under microgravity.

Keywords

calbindin-D28kimmunohistochemistryspace flightspinal cord
Type
Research Article
Information
International Journal of Astrobiology , Volume 14 , Issue 4 , October 2015 , pp. 555 - 562
Copyright
Copyright © Cambridge University Press 2015 

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