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Study of the effects of the outer space environment on dormant forms of microorganisms, fungi and plants in the ‘Expose-R’ experiment

Published online by Cambridge University Press:  19 January 2015

N. Novikova*
Affiliation:
RF SRC – Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
E. Deshevaya
Affiliation:
RF SRC – Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
M. Levinskikh
Affiliation:
RF SRC – Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
N. Polikarpov
Affiliation:
RF SRC – Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
O. Gusev
Affiliation:
Institute of Fundamental Medicine and Biology, Kazan Federal University, Russia Institute of Space and Astronautical Science, Japan
V. Sychev
Affiliation:
RF SRC – Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
*

Abstract

Investigations of the effects of solar radiation combined with the spaceflight factors on biological objects were performed in the «EXPOSE-R» experiment on the outer surface of ISS. After more than 1 year of outer space exposure, the spores of microorganisms and fungi, as well as two species of plant seeds were analysed for viability and the set of biological properties. The experiment provided evidence that not only bacterial and fungal spores but also dormant forms of plants had the capability to survive a long-term exposure to outer space.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2015 

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References

Atlas, R.M. & Parks, L.C. (1993). Handbook of Microbiological Media. CRC Press, Inc. London.Google Scholar
Baranov, V.M., Novikova, N.D., Polikarpov, N.A., Sychev, V.N., Levinskikh, M.A., Alekseev, V.R., Okuda, T., Sugimoto, M., Gusev, O.A. & Grigor’ev, A.I. (2009). The Biorisk experiment: 13-month exposure of resting forms of organisms on the outer side of the Russian Segment of the International Space Station. Preliminary results. Dokl. Biol. Nauk 426, 267270 (in Russian).CrossRefGoogle ScholarPubMed
Horneck, G. (1993). Responses of Bacillus subtilis spores to the space environment: results from experiments in space. Orig. Life Evol. Biosph. 23(1), 3752.CrossRefGoogle Scholar
Horneck, G., Klaus, D.M. & Mancinelli, R.L. (2010). Space microbiology. Microbiol. Mol. Biol. Rev. 74, 121156.CrossRefGoogle ScholarPubMed
Horneck, G., Moeller, R., Cadet, J., Douki, T., Mancinelli, R.L., Nicholson, W.L., Panitz, C., Rabbow, E., Rettberg, P., Spry, A., Stackebrandt, E., Vaishampayan, P. & Venkateswaran, K.J. (2012). Resistance of bacterial endospores to outer space for planetary protection purposes – experiment PROTECT of the EXPOSE-E mission. Astrobiology 12, 445456.CrossRefGoogle ScholarPubMed
Jeffries, C.D., Holtman, D.F. & Guse, D.G. (1957). Rapid method for determining the activity of microorganisms on nucleic acids. J. Bacteriol. 73(4), 590591.CrossRefGoogle ScholarPubMed
Labinskaya, A.S. & Volina, E.G. (2008). Sanitation Microbiology. Medical Microbiology Guidebook. Book 1: General and Sanitary Microbiology. Eds. BINOM, Moscow. pp. 350352 (in Russian).Google Scholar
La Duc, M.T., Nicholson, W., Kern, R. & Venkateswaran, K. (2003). Microbial characterization of Mars Odyssey spacecraft and its encapsulated facility. Environ. Microbiol. 5(10), 977985.Google Scholar
Novikova, N.D., Gusev, O., Polikarpov, N.A., Deshevaya, E.A., Levinskikh, M., Alekseev, V., Okuda, T., Sugimoto, M., Sychev, V. & Grigoriev, A. (2011). Survival of dormant organisms after long-term exposure to the space environment. Acta Astronaut. 68, 15741580.CrossRefGoogle Scholar
Potebnia, H.P., Safronova, L.A., Cheremshenko, N.L., Lisovenko, H.S., Sorokulova, I.B., Prykhod'ko, V.O., Trokhymenko, N.V., Tanasiienko, O.A. & Bombin, A.V. (2006). Influence of probiotic subalin on efficiency of antitumor vaccine. Mikrobiol. Z. 68(6), 5158.Google Scholar
Rabbow, E., Horneck, G., Rettberg, P., Schott, J.U., Panitz, C., L'Afflitto, A., von Heise-Rotenburg, R., Willnecker, R., Baglioni, P., Hatton, J., Dettmann, J., Demets, R. & Reitz, G. (2009). EXPOSE, an astrobiological exposure facility on the International Space Station—from proposal to flight. Orig. Life Evol. Biosph. 39, 581598.Google Scholar
Rabbow, E., Rettberg, P., Barczyk, S., Bohmeier, M., Parpart, A., Panitz, C., Horneck, G., von Heise-Rotenburg, R., Hoppenbrouwers, T., Willnecker, R., Baglioni, P., Demets, R., Dettmann, J. & Reitz, G. (2012). EXPOSE-E: an ESA astrobiology mission 1.5 years in space. Astrobiology 12, 374386.Google Scholar
Rabbowa, E., Rettberg, P., Barczyk, S., Bohmeier, M., Parpart, A., Panitz, C., Horneck, G., Burfeindt, J., Molter, F., Jaramillo, E., Pereira, C., Weiß, P., Willnecker, R., Demets, R., Dettmann, J. & Reitz, G. (2014). The astrobiological mission EXPOSE-R on board of the International Space Station. Int. J. Astrobiol. (this issue), Published online: 28 08 2014 , doi: http://dx.doi.org/10.1017/S1473550414000202 Google Scholar
Sugimoto, M., Oono, Y., Gusev, O., Matsumoto, T., Yazawa, T., Levinskikh, M.A., Sychev, V.N., Bingham, G.E., Wheeler, R. & Hummerick, M. (2014). BMC Plant Biol. 14(4). doi: 10.1186/1471-2229-14-4.CrossRefGoogle Scholar
Tepfer, D., Zalar, A. & Leach, S. (2012). Survival of plant seeds, their UV screens, and nptII DNA for 18 months outside the International Space Station. Astrobiology 12, 517528.Google Scholar
Vaishampayan, P.A., Rabbow, E., Horneck, G. & Venkateswaran, K.J. (2012). Survival of Bacillus pumilus spores for a prolonged period of time in real space conditions. Astrobiology 12, 487497.Google Scholar
Wassmann, M., Moeller, R., Rabbow, E., Panitz, C., Horneck, G., Reitz, G., Douki, T., Cadet, J., Stan-Lotter, H., Cockell, C.S. & Rettberg, P. (2012). Survival of spores of the UV-resistant Bacillus subtilis strain MW01 after exposure to low-Earth orbit and simulated martian conditions: data from the space experiment ADAPT on EXPOSE-E. Astrobiology 12, 498507.Google Scholar