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Purification procedure sensitizes Bacillus endospores to free radicals from UVA radiation and photocatalysis

Published online by Cambridge University Press:  03 August 2017

Vijay Krishna ,
Jue Zhao ,
Ben Koopman  and
Brij Moudgil
Vijay Krishna*
Affiliation:
Department of Materials Science and Engineering, Particle Engineering Research Center, University of Florida, P.O. Box-116135, Gainesville, Florida 32611, USA
Jue Zhao
Affiliation:
Department of Environmental Engineering Sciences, University of Florida, P.O. Box-116135, Gainesville, Florida 32611, USA
Ben Koopman
Affiliation:
Department of Environmental Engineering Sciences, University of Florida, P.O. Box-116135, Gainesville, Florida 32611, USA
Brij Moudgil
Affiliation:
Department of Materials Science and Engineering, Particle Engineering Research Center, University of Florida, P.O. Box-116135, Gainesville, Florida 32611, USA
*
e-mail: krishnv2@ccf.org
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Abstract

Many researchers are investigating the extreme resilience of bacterial endospores against chemical and physical inactivating agents. The presence of vegetative cells in spore suspensions can result in overly optimistic assessment of inactivating agents; therefore, various spore purification methods have been applied to separate spores from vegetative cells prior to testing. The present study was undertaken to evaluate the effect of two widely used spore purification methodologies on spore integrity and susceptibility to ultraviolet-A (UVA) radiation and free radicals generated from photocatalysts. Bacillus subtilis and Bacillus cereus spores were purified by procedures that involved heat shock alone or chemical washes, lysozyme treatment and heat shock (CLH). The purified spores were exposed to UVA radiation or free radicals generated by photocatalyst and susceptibility were evaluated in terms of survival ratio. The effect of purification procedure on the spore morphology was investigated with electron microscopy. The CLH purification process significantly damages spore coats and increases the susceptibility of Bacillus spores to UVA radiation and photocatalytic inactivation. It is therefore likely that the survival of CLH treated spores in extra-terrestrial environments would be less than that of the same spores purified by a less aggressive procedure.

Keywords

heat shocklysozymephotocatalysispost-harvestpurificationspores
Type
Research Article
Information
International Journal of Astrobiology , Volume 17 , Issue 4 , October 2018 , pp. 329 - 335
Copyright
Copyright © Cambridge University Press 2017 

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Footnotes

*

Present Address: Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.

†

Present address: Public Works Department, City of Salem, Salem, Oregon 97303, USA.

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