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Label-free RF biosensors for human cell dielectric spectroscopy

Published online by Cambridge University Press:  19 January 2010

Claire Dalmay ,
Arnaud Pothier ,
Mathilde Cheray ,
Fabrice Lalloue ,
Marie-Odile Jauberteau  and
Pierre Blondy
Claire Dalmay*
Affiliation:
XLIM Research Institute, Centre National de la Recherche Scientifique (CNRS), University of Limoges, 87060 Limoges Cedex, France.
Arnaud Pothier
Affiliation:
XLIM Research Institute, Centre National de la Recherche Scientifique (CNRS), University of Limoges, 87060 Limoges Cedex, France.
Mathilde Cheray
Affiliation:
Laboratory of “Homeostasie cellulaire et Pathologies”, University of Limoges, 87060 Limoges Cedex, France.
Fabrice Lalloue
Affiliation:
Laboratory of “Homeostasie cellulaire et Pathologies”, University of Limoges, 87060 Limoges Cedex, France.
Marie-Odile Jauberteau
Affiliation:
Laboratory of “Homeostasie cellulaire et Pathologies”, University of Limoges, 87060 Limoges Cedex, France.
Pierre Blondy
Affiliation:
XLIM Research Institute, Centre National de la Recherche Scientifique (CNRS), University of Limoges, 87060 Limoges Cedex, France.
*
Corresponding author: C. Dalmay Email: claire.dalmay@xlim.fr
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Abstract

This paper presents an original biosensor chip allowing determination of intrinsic relative permittivity of biological cells at microwave frequencies. This sensor permits non-invasive cell identification and discrimination using an RF signal to probe intracellular medium of biological samples. Indeed, these sensors use an RF planar resonator that allows detection capabilities on less than 10 cells, thanks to the microscopic size of its sensitive area. Especially, measurements between 15 and 35 GHz show the ability label-free biosensors to differentiate two human cell types using their own electromagnetic characteristics. The real part of permittivity of cells changes from 20 to 48 for the nervous system cell types studied. The proposed biodetection method is detailed and we show how the accuracy and the repeatability of measurements have been improved to reach reproducible measurements.

Keywords

BiosensorsBiomedical measurementsBiological cellMicrowave resonatorsMicrowave spectroscopy
Type
Original Article
Information
International Journal of Microwave and Wireless Technologies , Volume 1 , Special Issue 6: 2009 National Microwave Days in France , December 2009 , pp. 497 - 504
Copyright
Copyright © Cambridge University Press and the European Microwave Association 2010

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References

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