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Push–pull configuration of high-power MOSFETs for generation of nanosecond pulses for electropermeabilization of cells

Published online by Cambridge University Press:  27 May 2019

I. W. Davies ,
C. Merla ,
A. Casciati ,
M. Tanori ,
A. Zambotti ,
M. Mancuso ,
J. Bishop ,
M. White ,
C. Palego  and
C. P. Hancock
I. W. Davies*
Affiliation:
School of Computer Science and Electronic Engineering, University of Bangor, Bangor, UK Creo Medical, Bath, UK
C. Merla
Affiliation:
Division of Health Protection Technologies, ENEA-Casaccia, Rome 00123, Italy
A. Casciati
Affiliation:
Division of Health Protection Technologies, ENEA-Casaccia, Rome 00123, Italy
M. Tanori
Affiliation:
Division of Health Protection Technologies, ENEA-Casaccia, Rome 00123, Italy
A. Zambotti
Affiliation:
Division of Health Protection Technologies, ENEA-Casaccia, Rome 00123, Italy
M. Mancuso
Affiliation:
Division of Health Protection Technologies, ENEA-Casaccia, Rome 00123, Italy
J. Bishop
Affiliation:
Creo Medical, Bath, UK
M. White
Affiliation:
Creo Medical, Bath, UK
C. Palego
Affiliation:
School of Computer Science and Electronic Engineering, University of Bangor, Bangor, UK
C. P. Hancock
Affiliation:
School of Computer Science and Electronic Engineering, University of Bangor, Bangor, UK Creo Medical, Bath, UK
*
Author for correspondence: I. W. Davies, E-mail: eeu25b@bangor.ac.uk
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Abstract

A power MOSFET-based push–pull configuration nanosecond-pulse generator has been designed, constructed, and characterized to permeabilize cells for biological and medical applications. The generator can deliver pulses with durations ranging from 80 ns up to 1 µs and pulse amplitudes up to 1.4 kV. The unit has been tested for in vitro experiments on a medulloblastoma cell line. Following the exposure of cells to 100, 200, and 300 ns electric field pulses, permeabilization tests were carried out, and viability tests were conducted to verify the performance of the generator. The maximum temperature rise of the biological load was also calculated based on Joule heating energy conservation and experimental validation. Our results indicate that the developed device has good capabilities to achieve well-controlled electro-manipulation in vitro.

Keywords

Circuit design and applicationsEM field theory and numerical techniquesmedical and biological effectspassive components and circuitsSi-based devices and IC technologies
Type
EuMW 2018
Information
International Journal of Microwave and Wireless Technologies , Volume 11 , Special Issue 7: MIKON 2018 / EuMW 2018 (Part II) , September 2019 , pp. 645 - 657
Copyright
Copyright © Cambridge University Press and the European Microwave Association 2019 

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