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Effects of ionizing radiation on tsetse biology. Their relevance to entomological monitoring during integrated control programmes using the sterile insect technique

Published online by Cambridge University Press:  19 September 2011

A. M. V. Van der Vloedt  and
H. Barnor
A. M. V. Van der Vloedt
Affiliation:
Joint FAO/IAEA Division of Isotope and Radiation Applications of Atomic Energy for Food and Agricultural Development, International Atomic Energy Agency, Vienna, Austria
H. Barnor
Affiliation:
Joint FAO/IAEA Division of Isotope and Radiation Applications of Atomic Energy for Food and Agricultural Development, International Atomic Energy Agency, Vienna, Austria
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Abstract

The effects of gamma irradiation from a 60Co-source on Glossina palpalis palpalis (Robineau-Desvoidy) were established in laboratory tests. Dose-response data indicated that fecundity was reduced by about 95% following exposure of late stage puparia or young adults to 11–13 krad in air and 15–18 krad in a nitrogen atmosphere. None of the treatments had a detectable influence on the viability, mating behaviour, inseminating capability or competitiveness of the adult males.

When irradiated males were combined in varying ratios with untreated males and females, increasing numbers of sterile males resulted in a considerable reduction in pregnancy stages beyond the egg stage, an increasing proportion of parous females with an empty uterus due to expulsion of a dead embryo after embryonic arrest or due to ovary blockage and an increasing percentage of females with a degenerating egg in utero.

Remating of inseminated females with fertile males during the period when most of them had emptied their uterus (day 14–20) after embryonic arrest, had no noticeable effect on the sterility rate.

A most important consequence of recurring embryonic death was a detectable imbalance between intra-uterine and ovarian development. Some aberrations can be used as additional criteria for monitoring the effectiveness of sterile males released in the field.

Females irradiated in the pupal or adult stage displayed a normal receptivity and insemination rate and survived distinctly longer than untreated controls. Moreover, they built up abnormally abundant fat bodies and showed multiple mating scars (restricted to the palpalis group species) and completely atrophied ovaries.

It is postulated that the release-recapture and examination of the reproductive tract of irradiated females during an advanced stage of sterile release vector control operations would give evidence of mating that could expose the existence of relic wild populations, particularly when the natural tsetse population has been reduced to, levels below the threshold detectable by conventional techniques.

Keywords

Tsetsegamma irradiationsterile male releasesentomological monitoring
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 5 , Special Issue 5: Tsetse Behaviour and Population Ecology , October 1984 , pp. 431 - 437
Copyright
Copyright © ICIPE 1984

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References

REFERENCES

Challier, A. (1965) Amélioration de la méthode de détermination de l'âge physiologique des glossines. Bull. Soc. Path. exot. 58, 250–259.Google Scholar
Challier, A. and Laveissière, C. (1973) Un nouveau piège pour la capture des glossines (Glossina; Diptera, Mus-cidae): description et essais sur le terrain. Cah. ORSTOM Sér. ent. méd. Parasit. 11, 251–262.Google Scholar
Cuisance, D., Politzar, H., Clair, M., Sellin, E., Tazé, Y., Bourdoiseau, G. and Février, J. (1980) La lutte contre Glossina palpalis gambiensis Vanderplank par lâchers de mâles irradiés en Haute-Volta. Etude de paramètres opérationnels. Proc. Symp. Vienna, May 1979, Joint FAO/IAEA, IAEA-SM-240/2, pp. 249–266.Google Scholar
Curtis, C. F. and Langley, P. A. (1972) Use of nitrogen and chilling in the production of radiation-induced sterility in the tsetse fly Glossina morsitans. Entomologia exp. appi. 15, 360–376.CrossRefGoogle Scholar
Curtis, C. F. (1980) Factors affecting the efficiency of the sterile insect release method for tsetse. Proc. Symp. Vienna, May 1979, Joint FAO/IAEA, IAEA-SM-240/25, pp. 381–396.Google Scholar
Dame, D. A., Williamson, D. L., Cobb, P. E., Gates, D. B., Warner, P. V., Mtuya, A. G. and Baumgartner, H. (1980) Integration of sterile insects and pesticides for the control of the tsetse fly Glossina morsilans morsitans. Proc. Symp. Vienna, May 1979, Joint FAO/IAEA, IAEA-SM-240/4, pp. 267–280.Google Scholar
Jackson, C. H. (1946) An artificially isolated generation of tsetse flies (Diptera). Bull. ent. Res. 37, 291–299.CrossRefGoogle ScholarPubMed
Langley, P. A., Curtis, C. F. and Brady, J. (1974) The viability, fertility and behaviour of tsetse flies (Glossina morsitans) sterilized by irradiation under various conditions. Entomologia exp. appl. 17, 97–115.CrossRefGoogle Scholar
Saunders, D. S. (1960) The ovulation cycle in Glossina morsitans and a possible method of age determination for female tsetse flies by examination of their ovaries. Trans. R. ent. Soc. Lond. 112, 221–223.CrossRefGoogle Scholar
Van der Vloedt, A. M. V. (1982) Recent advances in tsetse mass-rearing with particular reference to Glossina palpalis palpalis fed in vivo on guinea pigs. In Sterile Insect Technique, IAEA-SM-255/11, pp. 223–253.Google Scholar
Wetzel, H. and Luger, D. (1978) In vitro feeding in the rearing of tsetse flies (Glossina m. morsitans and G. p. palpalis, Diptera: Glossinidae). Tropenmed. Parasit. 29, 239–251.Google Scholar