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Activity of digestive enzymes in Zonocerus variegatus (Orthoptera: Pyrgomorphidae) gut homogenates during post-embryonic development

Published online by Cambridge University Press:  30 March 2011

K.O. Ademolu  and
A.B. Idowu
K.O. Ademolu*
Affiliation:
Biological Sciences Department, University of Agriculture, P.M.B. 2240, Abeokuta, Ogun State, Nigeria
A.B. Idowu
Affiliation:
Biological Sciences Department, University of Agriculture, P.M.B. 2240, Abeokuta, Ogun State, Nigeria
*
*E-mail: kennyademolu@yahoo.com
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Abstract

The activity of five enzymes – amylase, α-glucosidase, cellulase, proteinase and lipase – present in the foregut, midgut and hindgut of the African variegated grasshopper Zonocerus variegatus (L.) was studied in the post-embryonic stages of the insect. The activities of all the enzymes increased with age of the insect, with the adult stage having significantly higher (P < 0.05) activity than the lower instars. The midgut had the highest activity for all the enzymes except for amylase. Proteinase activity was the highest, and cellulase the lowest in the gut regions. These findings indicate that the adult stage of the polyphagous insect has higher digestive capacity than the immature stages.

Keywords

Zonocerus variegatusdigestiongut enzymesdevelopment
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 1-2 , June 2011 , pp. 29 - 33
Copyright
Copyright © ICIPE 2011

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References

Adedire, C. O. and Balogun, R. A. (1995) Digestive enzymes and regional localization of proteolytic endopeptidases in alimentary canal of kola nut weevil, Sophrorhinus insperatus Faust (Coleoptera: Curculionidea). Entomon 20, 183–189.Google Scholar
Ademolu, K. O., Idowu, A. B. and Amusan, A. A. S. (2007) Chemical analysis of tissues of Zonocerus variegatus (L) (Orthoptera: Pyrgomorphidae) during post-embryonic development in Abeokuta, South-western, Nigeria. Nigerian Journal of Entomology 24, 24–34.CrossRefGoogle Scholar
Ademolu, K. O., Idowu, A. B. and Dansu, B. M. (2006) Morphometric analysis of Zonocerus variegatus during post-embryonic development, pp. 75–79. In Proceedings of the Second International Conference on Science and National Development organized by the College of Natural Sciences. University of Agriculture, Abeokuta, Nigeria.Google Scholar
Ademolu, K. O., Idowu, A. B. and Olatunde, G. O. (2009) Morphometrics and enzyme activities in the femoral muscles of variegated grasshopper, Zonocerus variegatus (Orthoptera: Pyrgomorphidae) during post embryonic development. International Journal of Tropical Insect Science 29, 53–56.CrossRefGoogle Scholar
Anya, A. O. (1973) Ecology of the variegated grasshopper, Zonocerus variegatus (Orthoptera, Acridoidea, Pyrgomorphidae) on the Nsukka Plateau. Entomologia Experimentalis et Applicata 16, 65–76.CrossRefGoogle Scholar
Apori, L., Castro, O. and Orskov, M. (2000) Chemical composition and nutritive value of leaves and stems of tropical weed Chromolaena odorata. Grass and Forage Science 55, 77.CrossRefGoogle Scholar
Ayodeji, O. F. and Valentine, A. A. (2005) Varietal composition and functional properties of cassava (Manihot esculenta Crantz) leaf meal and leaf protein concentrates. Pakistan Journal of Nutrition 4, 43–49.Google Scholar
Balogun, R. A. (1972) Digestive carbohydrases and the nature of amylase in the gut homogenates of Zonocerus variegatus (L.). Bulletin of the Entomological Society of Nigeria 3, 91–94.Google Scholar
Bernays, E. A., Chapman, R. F., Cook, A. G., Meveigh, L. J. and Page, W. M. (1975) Food plants in the survival and development of Zonocerus variegatus (L). Acrida 4, 33–45.Google Scholar
Centre for Overseas Pest Research London (COPR) (1974) Control of Zonocerus variegatus (L) in Nigeria. Interim Report: 1972–1973. ODM Research Scheme R2727.Google Scholar
Chapman, R. (1990) The Insect: Structure and Function. The English Language Book Society and Hodder and Stoughton, Great Britain. 968 pp.Google Scholar
Chapman, R. F., Page, W. W. and McCaffery, A. R. (1986) Bionomics of the variegated grasshopper Zonocerus variegatus in West and Central Africa. Annual Review of Entomology 32, 479–505.CrossRefGoogle Scholar
Clissold, F. J., Sanson, G. D. and Read, J. (2004) Indigestibility of plant cell wall by the Australian plague locust, Chortoicetes terminifera. Entomologia Experimentalis et Applicata 112, 159–168.CrossRefGoogle Scholar
Dow, J. A. T. (1981) Counter current flows, water movements and nutrient absorption in the locust midgut. Journal of Insect Physiology 27, 79–85.CrossRefGoogle Scholar
Englemann, F. (1969) Food-stimulated synthesis of intestinal proteolytic enzymes in the cockroach Leucophaea maderae. Journal of Insect Physiology 10, 657–674.Google Scholar
Hill, S. R. and Orchard, I. (2005) Enzymes in the gut during starvation. Journal of Insect Physiology 51, 1–9.CrossRefGoogle Scholar
Idowu, A. B. and Edema, M. O. (2005) The microbial flora of the different gut regions of Zonocerus variegatus (L) (Orthoptera: Pyrgomorphidae). Nigerian Journal of Plant Protection 20, 19–30.Google Scholar
Idowu, A. B. and Sonde, O. A. (2004) The contribution of food plants to the growth, development and fecundity of Zonocerus variegatus (L). Nigerian Journal of Entomology 21, 24–28.CrossRefGoogle Scholar
Idowu, A. B., Edema, M. O. and Oyedepo, M. T. (2009) Extracellular enzyme production by microflora from the gut region of the variegated grasshopper Zonocerus variegatus (Orthoptera: Pyrgomorphidae). International Journal of Tropical Insect Science 29, 229–235.CrossRefGoogle Scholar
Jianchu, M. O., Tianci, Y., Xiaogang, S. and Jiaan, C. (2004) Cellulase activity in five species of important termites in China. Applied Entomology and Zoology 39, 635–641.Google Scholar
Lebene, M. J. (1998) The foregut microscopy, pp. 713–724. In Microscopic Anatomy of Invertebrates vol. 11B, Insecta (edited by Harrison, F. W. and Locke, M.). Wiley-Liss, New York.Google Scholar
Mandal, S., Roy, S. and Chaudhuri, D. K. (1981) Quantification of protease, amylase, and lipase in the gut of Schizodactylus monstrosus during the post-embryonic development stage. Effect of starvation, stress and topical application of juvenile hormone analogue. Acta Physiologica Academiae Scientiarum Hungaricae 58, 53–64.Google Scholar
Modder, W. W. D. (1984) Diurnal variation in the feeding and growth activity in nymphs of the African pest, grasshopper Zonocerus variegatus (L). Insect Science and Its Application 5, 527–531.Google Scholar
Muse, W. A. (2003) Effect of sex, age, starvation and feeding, isolation and crowding and oviposition on longevity of Zonocerus variegatus (L) (Orthoptera: Pyrgomorphidae). Journal of Biological Research and Biotechnology 1, 11–20.Google Scholar
Obidapo, O. (1979) Studies on gut proteolytic enzymes and aspects of digestive physiology in the African pest grasshopper, Zonocerus variegatus (L) (Acridoidea: Pyrgomorphidae). PhD thesis, University of Ibadan. 452 pp.Google Scholar
Terra, W. R. (1990) Evolution of digestive systems of insects. Annual Review of Entomology 25, 181–200.CrossRefGoogle Scholar
Toye, S. A. (1982) Studies on the biology of the grasshopper pest Zonocerus variegatus (L) (Orthoptera: Pyrgomorphidae) in Nigeria. Insect Science and Its Application 3, 1–7.Google Scholar
Wigglesworth, V. R. (1963) A further function of the air sacs in some insects. Nature 198, 106.CrossRefGoogle Scholar
Woodring, J., Hoffman, K. and Lorenz, M. W. (2007) Activity, release and flow of digestive enzymes in the cricket Gryllus bimaculatus. Physiological Entomology 32, 56–63.CrossRefGoogle Scholar