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Effects of anti-juvenile hormone agents and a juvenile hormone analog on neck-ligated post-feeding last instar larvae of Spodoptera mauritia Boisd. (Lepidoptera:Noctuidae)

Published online by Cambridge University Press:  19 September 2011

E. Balamani  and
V. S. K. Nair
E. Balamani
Affiliation:
Department of Zoology, University of Calicut, Kerala, India673 635
V. S. K. Nair
Affiliation:
Department of Zoology, University of Calicut, Kerala, India673 635
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Abstract

The ability of three anti-juvenile hormone agents (AJHAs) to prevent the juvenile hormone dependent phase of larval-pupal transformation has been investigated. Neck-ligated post-feeding last instar larvae of Spodoptera mauritia were treated topically with different doses of a juvenile hormone analog (JHA) or the AJHAs, tetra-hydro-4-fluoromethyl-4-hydroxy-2H-pyran-2-one (FMev), ethyl-4-[2-(tert-butyl carbonyloxy)butoxy] benzoate (ETB) and ethyl-[E]-3-methyl-2-dodecanoate (EMD). Untreated ligated larvae or those treated with acetone survived for 12 ± 1 days without showing any sign of pupal cuticle secretion. On the other hand treatments of ligated larvae with different doses of JHA induced pupation. Among the three AJHAs tested treatments of ETB promoted pupation. Treatments of ligated larvae with lower doses of EMD induced the formation of larval-pupal intermediates whereas those treated with higher dose moulted into either pupae or larval-pupal intermediates. FMev-treated larvae exhibited a complete inhibition of moulting and metamorphosis. In order to study whether AJHAs would prevent the JHA induced pupation, neck-ligated larvae were treated simultaneously with different doses of AJHAs and 1 μg JHA. Co-application of JHA with different doses of EMD induced pupation in majority of the ligated larvae and thus appears to a certain extent to counteract the effects of treatments of same doses of EMD alone. Co-application of JHA with different doses of ETB elicited pupation in all the surviving ligated larvae. To a large extent co-application of JHA with different doses of FMev failed to induce pupation. The significance of the findings is discussed in the context of the mode of action of these AJHAs.

Résumé

Les trois (AJHAs) ont été examinés son pouvoir qu' empeche la transformation du Juvenile hormone dependent phase de larvaere-pupae. Ayant nourir dernière stade cou-ligaturés larvaire de Spodoptera mauritia sont traité topicalement avec en doses differements du Juvenile hormone analog (JHA) ou AJHAs, tetra-hydro-4-fluoromethyl-4-hydroxy-2H-pyran-2-one (FMev), ethyl-4-[2-(tert-butyl carbonyloxy)butoxy] benzoate (ETB) et ethyl-[E]-3-methyl 2-dodecanoate (EMD). Non traité larvaire ligaturés ou eel les sont traité avec acetone survie pendant 12 ± 1 jours sans aucune signe de secretion par pupal cuticle. D'ailleurs traitement des larvaires ligaturés avec en doses differents de JHA induit pupation. Parmi les larvaire déjà traité par trois AJHAs sont induit pupation par ETB. Traitment des larvaires ligaturés avec en doses inferieurs du EMD induit la formation de larvaires-pupal intermediares tandisque celles qui sont traités en doses superieurs mue a pupae ou larvaires pupal intermediares. Larvaires traités par FMev montre une inhibition complete du mue et metamorphosis. A fin d'étudier si AJHAs empechevont JHA induit pupation, cou-ligaturé larvaires sont traité simultanement avec en doses differents des AJHAs et 1 μg JHA. En majorité des larvaires ligaturés induit pupation par co-traitment de JHA avec en doses differents de EMD. Co-traitment de JHA avec en doses differents de ETB elicit pupation dans toutes les larvaires ligaturés survivant. En plus part des cas co-traitment de JHA avec en doses differents de FMev n'induit pas la pupation. Les significances des resultats ont discutes d'une mannière de la mode d'action des AJHAs.

Keywords

Spodoptera mauritiaanti-juvenile hormone agentsmode of actioninsect metamorphosis
Type
Articles
Information
International Journal of Tropical Insect Science , Volume 10 , Issue 5 , October 1989 , pp. 583 - 589
Copyright
Copyright © ICIPE 1989

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