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Gut microflora of the larva of silkworm, Bombyx mori

Published online by Cambridge University Press:  19 September 2011

S. Kalpana ,
A. A. M. Hatha  and
P. Lakshmanaperumalsamy
S. Kalpana
Affiliation:
Department of Environmental Sciences, Bharathiar University, Coimbatore-641 046, India
A. A. M. Hatha
Affiliation:
Department of Environmental Sciences, Bharathiar University, Coimbatore-641 046, India
P. Lakshmanaperumalsamy
Affiliation:
Department of Environmental Sciences, Bharathiar University, Coimbatore-641 046, India
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Abstract

The quantitative and qualitative changes of bacterial flora associated with the silkworm (Bombyx mori) at different stages of its life cycle were studied. Larvae reared on mulberry leaves were dissected and their bacterial populations counted after a 3-day incubation period at 28 ± 2°C. The results showed a higher heterotrophic bacterial population in the fourth and the fifth instars, which coincide with the active feeding stage of the larvae. The similarity of the genera observed in the digestive tract and that observed on the leaves fed to the larvae suggests colonisation of food microflora in the gut following ingestion. The bacteria inhabiting the gut of silkworm were found to be elaborating amylase, caseinase, gelatinase, lipase and urease. The highest percentage of isolates were protease producers followed by lipid and polysaccharide splitters. The results indicate that the bacterial flora play an important role in the digestion of ingested food material.

Résumé

Des changements quantitatifs et qualitatifs de la flore bacérienne associée au ver-à-soie ont été étudiés à différents stades de son cycle de vie. Des larves elevées sur des feuilles du mûrier ont été disséquées et leur flore bactérienne a été comptée après 3 jours d'incubation à 28 ± 2°C. Les résultats montrent une population bactérienne hautement hétérotrophique chez les 4ème et 5ème stades qui coïncident aux stades nourriciers de la larve. La similarité des genres observés au niveau du tractus digestif et sur les feuilles suggére la colonisation de la microflore de la nourriture au niveau des intestins par suite de l'ingestion. On a trouvé que les bactéries peuplant les intestins du ver-à-soie élaborent l'amylase, la caséinase, la gélatinase, la lipase et l'uréase. Un plus grand pourcentage des isolats ont été des producteurs de protéase, suivis de séquenceurs de lipides et de polysaccharides. Les résultats indiquent que la flore bactérienne joue un rôle important dans la digestion de la nourriture ingérée.

Keywords

silkwormmulberry leavesbacterial populationhydrolytic formsver-à-soiefeuilles du mûrierflore bactérienneformes hydrolytiques
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 15 , Issue 4-5 , October 1994 , pp. 499 - 502
Copyright
Copyright © ICIPE 1994

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References

REFERENCES

Aizawa, K., Furuea, Y., Kurata, K. and Sato, F. (1964) On the etiologic agent of the infectious flacherie of the silkworm, Bombyx mori L. Bull. Seric. Exp. Stn. 19, 240.Google Scholar
Anitha, S., Meena, T. P. and Vanitha, Rani R. (1992) The bacterial pathogen of silkworm, Bombyx mori. 32nd Annual Conference of Association of Microbiologists in India. Madurai, 25 pp.Google Scholar
Bignell, D. E. (1984) The arthropod gut as an environment for microorganisms. Joint symposium of the British Mycological Society and the British Ecological Society, University of Excites, Chapter 8, pp. 205–227.Google Scholar
Brooks, M. A. (1975) Symbiosis and attenuation. Ann. N. Y. Acad. Sci. 266, 166–172.CrossRefGoogle ScholarPubMed
Buchanan, R. E. and Gibbons, N. E. (1979) Bergey's Manual of Determinative Bacteriology, 8th ed.Williams and Willkins, Baltimore, Maryland.Google Scholar
Chitra, D., Karanth, N. G. K. and Vasantharajan, U. N. (1975) Diseases of mulberry silkworm, Bombyx mori L. Biochem. Rev. 56, 44–61.Google Scholar
Cowan, S. T. and Steel, K. J. (1970) Manual for the Identification of Medical Bacteria. Cambridge University Press, London & New York.Google Scholar
Cruden, D. L. and Markovetz, A. J. (1979) Carboxymethyl cellulose decomposition by intestinal bacteria of cockroaches. Appl. Environ. Microbiol. 38, 369–372.CrossRefGoogle ScholarPubMed
Edwards, P. R. and Ewing, W. H. (1972) Identification of Enterobacteriaceae. 3rd ed.Burgess Publishing Co. Minnesota.Google Scholar
Harris, J. M., Seiderer, L. J. and Lucas, M. I. (1991) Gut microflora of two saltmarsh detritivore thalassinid prawns, Upogebia africana and Callianssa kussi. Microbial. Ecol. 21, 277–296.CrossRefGoogle Scholar
Manchev, M., Doneea, M. and Donew, B. (1984) Attempts to use therapeutic agents against diseases in silkworm moth, Bombyx mori L. Vet. Med. Nauk. 13, 95–102.Google Scholar
O'Brien, R. W. and Slaytor, M. (1982) Role of microorganisms in the metabolism of termites. Aust. J. Biol. Sci. 35, 239–262.CrossRefGoogle Scholar
Plante, C. J., Jumars, P. A. and Baross, J. A. (1989) Rapid bacterial growth in the hind gut of a marine deposit feeder. Microbial. Ecol. 18, 29–44.CrossRefGoogle Scholar
Steinhaus, E. A. (1963) Insect Pathology. Vol. 1 Acad. Press, New York & London.Google Scholar
Steinhaus, E. A. (1967) Insect Microbiology. Harnes Publishing Company, New York.CrossRefGoogle Scholar
Yamasaki, H. and Yamada, T. (1962) Studies on infectious flacherie (F-type) of silkworms, Bombyx mori. Bull. Nagano. Ken Sericult. Exp. Stn. 65, 1–13.Google Scholar