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Identification and molecular analysis of mid-gut mucin gene in Anopheles stephensi (Diptera: Culicidae)

Published online by Cambridge University Press:  16 March 2016

Nahid Borhani Dizaji ,
Irene Ricci ,
Guido Favia ,
Claudia Damiani ,
Hamid Reza Basseri ,
Mansour Heidari ,
Saied Reza Naddaf  and
Fatemeh Fotouhi
Nahid Borhani Dizaji
Affiliation:
Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
Irene Ricci
Affiliation:
School of Biosciences and Biotechnology, University of Camerino, Camerino, Italy
Guido Favia
Affiliation:
School of Biosciences and Biotechnology, University of Camerino, Camerino, Italy
Claudia Damiani
Affiliation:
School of Biosciences and Biotechnology, University of Camerino, Camerino, Italy
Hamid Reza Basseri
Affiliation:
Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mansour Heidari
Affiliation:
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Saied Reza Naddaf
Affiliation:
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
Fatemeh Fotouhi*
Affiliation:
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
*
*E-mail: fotouhi@pasteur.ac.ir
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Abstract

Recognition of Anopheles mid-gut molecules interacting with the malaria parasite is important as they can potentially be targeted to interrupt the life cycle of the parasite in the mosquito's body. The mid-gut of mosquitoes is covered with the glycocalyx, which is composed of various glycoproteins. Pieces of evidence show that mucin proteins are one of the most frequent ingredients of the glycocalyx. In the present study, we isolated and identified the sequence of mucin from the mid-gut of Anopheles stephensi, Liston and Kazerun strains. Anopheles stephensi mucin (AsMuc) has two central core repeats with the consensus sequence TTTTVAP flanked with a hydrophobic N-terminus and a C-terminus which it seems are both necessary for cell surface expression. To show if this molecule is expressed on the surface of the cell, we cloned AsMuc in a baculovirus vector and tracked the expression of the protein in Sf9 insect cells. Immune assays showed the surface localization of the recombinant mucin. AsMuc expression on the surface of the cell suggests it could be a potential ligand for Plasmodium spp. attachment to the Anopheles mid-gut.

Keywords

Anopheles stephensimucin genemolecular analysislocalization
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 36 , Issue 2 , June 2016 , pp. 60 - 69
Copyright
Copyright © icipe 2016 

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