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Integrity of the 130 kda polypeptide of Bacillus Thuringiensis SSP. Israelensis δ-Endotoxin in K-S sporulation medium

Published online by Cambridge University Press:  19 September 2011

Z. Ngalo Otieno-Ayayo ,
David M. Chipman ,
Arieh Zaritsky  and
Kamal Khawaled
Z. Ngalo Otieno-Ayayo
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P.O. Box 653, Beer Sheva 84105, Israel
David M. Chipman
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P.O. Box 653, Beer Sheva 84105, Israel
Arieh Zaritsky
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P.O. Box 653, Beer Sheva 84105, Israel
Kamal Khawaled
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev P.O. Box 653, Beer Sheva 84105, Israel
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Abstract

The 130 kDa polypeptide appears to be the most susceptible of the polypeptides constituting the Bacillus thuringiensis ssp. israelensis δ-endotoxin to the proteases associated with sporulation. Stabilization of this polypeptide in the recently described K-S sporulation medium has been achieved through limiting its cleavage by removing contaminating proteases from the culture by washing. In this way, the complete δ-endotoxin profile is retained for studies on all of the constituent polypeptide fractions.

Résumé

Parmi les polypeptides constituant δ-endotoxine de Bacillus thuringiensis israelensis, le polypeptide de poids moléculaire 130 kDa semble étre le plus vulnérable aux proteases associées à la sporulation. La stabilisation de ce polypeptide par le procéde récemment décrit, “milieu de sporulation K-S”, a été obtenue en limitant sa dégradation par lavage des protéase ayant contaminées de milieu de culture. Ainsi, le profile complet de δ-endotoxine a été retenu par études sur toutes les composantes des polypeptides.

Keywords

Bacillus thuringiensis israelensis130 kDa polypeptidedisappearance and stabilizationK-S mediumBacillus ssp. thuringiensis israelensispolypeptide deolypeptide de poids moléculaire 130 kDadisparition et stabilisationmilieu K-S
Type
Research Artilces
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 3 , June 1993 , pp. 377 - 381
Copyright
Copyright © ICIPE 1993

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References

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