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A laboratory technique for infecting adult tsetse with a fungal pathogen

Published online by Cambridge University Press:  19 September 2011

Nguya K. Maniania
Nguya K. Maniania
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
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Abstract

A technique for infecting adult tsetse with an entomopathogenic fungus was developed and tested in the laboratory. A nitrocellulose filter membrane (Millipore) was used as substrate for retaining conidia after fitration, and a cylindrical plastic tube served as a chamber for the flies. The technique was designed to allow accurate estimation of inoculum on the substrate, thereby permitting comparison of virulence during the screening of fungal pathogens against tsetse flies. Glossina morsitans centralis adults were susceptible to Metarhizium anisopliae at all doses tested, and mortalities were dose dependent. No significant differences in mortalities were observed when flies were exposed for different durations to the pathogen. The number of conidia picked up by flies varied considerably with exposure time and concentration.

Résumé

Une technique pour contaminer la mouche tsé-tsé avec un champignon entomopathogène a été mise au point puis testée au laboratoire. Une membrane filtrante en ester de cellulose (Millipore) a été utilisée comme substrat pour retenir les conidies après filtration, tandis qu'un tube cylindrique en plastique a servi pour chambre d'élevage pour les mouches. La technique a été conçue pour une estimation correcte de l'inoculum sur le substrat, ainsi permettant une comparaison de virulence entre les souches fongiques pour la lutte biologique contre la mouche tsé-tsé. Les adultes de Glossina morsitans centralis étaient susceptibles au Metarhizium anisopliae à toutes les doses éprouvées, et la mortalité était dose dépendante. La mortalité n'était pas significativement différente lorsque les mouches étaient exposées à l'inoculum pendant un temps variable. Le nombre de conidies collectées par les insectes variait considérablement en fonction du temps d'exposition et la concentration du pathogène.

Keywords

Glossina morsitans centralisMetarhizium anisopliaeinoculation techniquelaboratoryentomopathogenic fungusbiological controlGlossina morsitans centralisMetarhizium anisopliaetechnique d'inoculationlaboratoirechampignon entomopathogènelutte biologique
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 15 , Issue 4-5 , October 1994 , pp. 421 - 426
Copyright
Copyright © ICIPE 1994

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