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Mediation of inducible nitric oxide and immune-reactive lysozymes biosynthesis by eicosanoid and biogenic amines in flesh flies

Published online by Cambridge University Press:  10 January 2018

Amr A. Mohamed ,
Mona M. Ali ,
Moataza A. Dorrah  and
Taha T. M. Bassal
Amr A. Mohamed
Affiliation:
Department of Entomology, Faculty of Science, Cairo University, Giza, PO Box 12613, Egypt
Mona M. Ali
Affiliation:
Department of Entomology, Faculty of Science, Cairo University, Giza, PO Box 12613, Egypt
Moataza A. Dorrah
Affiliation:
Department of Entomology, Faculty of Science, Cairo University, Giza, PO Box 12613, Egypt
Taha T. M. Bassal*
Affiliation:
Department of Entomology, Faculty of Science, Cairo University, Giza, PO Box 12613, Egypt
*
*E-mail: taha@sci.cu.edu.eg
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Abstract

Nitric oxide (NO) plays various roles in insect immunity: as a cytotoxic component and as a signalling molecule; and immune-reactive lysozymes (IrLys) provide a first line of humoral immune functions against invading bacteria. Although there is considerable literature on eicosanoid and biogenic monoamine actions on insect immunity, there is no information on the role(s) of these chemicals in inducing NO and IrLys. We addressed this gap by challenging third instar Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy) with the Gram-positive bacterium Micrococcus luteus. Here, we report that bacterial challenge induces elevation of NO and IrLys concentrations in haemocytes and in the fat body. The plasma pool content is comparatively low. Eicosanoid biosynthesis inhibitors (EBIs) lead to suppression of both NO and IrLys levels. Control larvae have low constitutive levels of NO and lysozyme concentrations. Octopamine (OA) elicits elevation of NO and IrLys concentrations. A similar effect is obtained by 5-hydroxytryptamine (5-HT) for NO. These data indicate immune-mediating roles of eicosanoids, OA and 5-HT in NO and IrLys activities.

Keywords

Nitric oxidelysozymeseicosanoidsbiogenic monoaminesSarcophaga
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 38 , Issue 1 , March 2018 , pp. 93 - 104
Copyright
Copyright © icipe 2018 

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Footnotes

†

Both authors contributed equally to this work.

Authors' contributions

Conceptualization: TTMB, MAD and AAM. Data curation: TTMB and AAM. Formal analysis: MMA, AAM and MAD. Funding acquisition: MMA and AAM. Investigation: AAM and MMA. Methodology: TTMB, AAM and MAD. Resources: MAD, MMA and AAM. Software and Statistics: AAM. Supervision: TTMB and MAD. Validation: TTMB, AAM and MAD. Visualization: MMA and AAM. Writing – original draft: TTMB and AAM. Writing – review and editing: TTMB and AAM.

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