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Protein Polymorphism in Two Populations of the Brown Ear Tick, Rhipicephalus appendiculatus Neumann (Acari: Ixodidae)

Published online by Cambridge University Press:  19 September 2011

F. N. Baliraine ,
E. O. Osir ,
S. B. Obuya  and
F. J. Mulaa
F. N. Baliraine
Affiliation:
Intemational Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
E. O. Osir
Affiliation:
Intemational Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
S. B. Obuya
Affiliation:
Intemational Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya
F. J. Mulaa
Affiliation:
Department of Biochemistry, University of Nairobi, P. O. Box 30197, Nairobi, Kenya
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Abstract

Two-dimensional gel electrophoresis was used to compare the protein profiles of two geographically isolated populations of the tick Rhipicephalus appendiculatus Neumann (Acari: Ixodidae) in Kenya. Most of the protein spots were common to both populations, but a number were specific to each population. Since proteins are encoded by genes, the presence of population-specific proteins suggests that there may be genetic differences between the two populations. It is proposed that some of these population-specific proteins might be related to the differences in susceptibility of the ticks to Tlwileria pnrva infection.

Résumé

L'électrophorèse à gel bi-dimensionnel a été utilisée pour comparer les profiles protéjques de deux populations géographiquement isolées de tiques Rhipicephalus appendiculatus Neumann (Acari: Ixodidae) au Kenya. Bien que la plupart des taches protéiques étaient communes aux deux populations, certaines d'entre elles étaient cependant spécifiques à l'une ou l'autre population. Etant donné que les protéines sont codées par géne, la présence de protéines spécifiques à une population suggère l'existence possible de différences spécifiques entre ces deux populations. Ces protéines spécifiques pourraient être liées, en quelque sort, aux différences de prédisposition des tiques aux infections de Tlicileria parva.

Keywords

Rhipicephalus appendiculatusEast Coast fevertwo-dimensional gel electrophoresisisoelectric focusingampholinesRhipicephalus appendiculatusTheileria parvaélectrophorèse à gel bi-dimenskinnellemise au point isoélectriqueampholines
Type
Short Communications
Information
International Journal of Tropical Insect Science , Volume 20 , Issue 3 , September 2000 , pp. 227 - 231
Copyright
Copyright © ICIPE 2000

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