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Relative Activity of Various Esterases in Six Pakistani Strains of the Lesser Grain Borer, Rhyzopertha dominica (Fabricius)

Published online by Cambridge University Press:  19 September 2011

Abdul Rauf Shakoori ,
Khawaja Abdul Mujeeb ,
Shazia Maqbool  and
Syed Shahid Ali
Abdul Rauf Shakoori
Affiliation:
Cell and Molecular Biology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore-54590, Pakistan
Khawaja Abdul Mujeeb
Affiliation:
Cell and Molecular Biology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore-54590, Pakistan
Shazia Maqbool
Affiliation:
Cell and Molecular Biology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore-54590, Pakistan
Syed Shahid Ali
Affiliation:
Cell and Molecular Biology Laboratory, Department of Zoology, University of the Punjab, New Campus, Lahore-54590, Pakistan
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Abstract

Acetylcholinesterase (AChE), arylesterase (AE), carboxylesterase (CE), and cholinesterase (ChE) activities were determined in six adult populations of the lesser grain borer, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) collected from grain warehouses in various areas of Punjab, namely Lahore (L), Sialkot (S), Wazirabad (W), Multan (M), Chkhawatni (C), and Karachi (K). The multiple forms of esterases were resolved by polyacrylamide gel electrophoresis (PAGE) and activities determined biochemically, using a spectrophotometer. All the strains had the same AChE activity except Sialkot strain, which had 46% more activity than the susceptible K strain. Similarly K, S, W, L, and C strains exhibited almost the same AE level, whereas the M strain showed 29% lower activity than K strain. The CE activity of L (352%), S (198%) and C (214%) strains and ChE activity of L (178%) and C (317%) strains were significantly higher than that of K strain. The total esterases of L, S and C strains showed respectively 152%, 63% and 73% higher activity than the susceptible K strain. The PAGE pattern of various esterases coincided with the biochemical analysis. The variable thickness of the bands in the gel indicated relative esterase induction that could be correlated with the development of resistance in R. dominica.

Résumé

Les activités acétylcholinestérasique, arylestérasique, carboxylestérasique, et cholinestérasique ont été déterminées dans six populations d'adultes du foreur de grain, Rliyzopertlia dominica (F.), collectés à partir des entrepôts de grain dans diverses régions du Punjab, à savoir Lahore (L), Sialkot (S), Wazitabad (W), Multan (M), Chkhawatni (C), et Karachi (K). Diverses formes d'estérases ont été résolues par électrophorèse à gel de polyacrylamide et les activités biochimiquement déterminées par spectrophotomètrie. Toutes les souches avaient la mê;me activité acétylcholinestérasique, excepté la souche S, qui a eu 46% de plus d'activité que la souche K sensible. Les souches K, S, W, L, et C ont montré presque le même niveau d'arylestérase, tandis que la souche M avait 29% de moins d'activité que la souche K. L'activité carboxylestérasique des souches L (352%), S (198%) et C (214%) et l'activité cholinestérasique des souches L (178%) et C (317%) étaient sensiblement plus élevées que celles de la souche K. Toutes les estérases des souches L, S et C ont respectivement montré 152%, 63% et 73% d'activité plus élevée que la souche K sensible. La configuration de l'électrophorèse à gel de polyacrylamide de diverses estérases a coïncidé avec leurs analyses biochimiques. La variabilité de l'épaisseur des bandes dans le gel a suggeré l'induction relative d'estérase qui pourrait ê;tre corrélée avec le développement de la résistance au sein des pupulations de R. dominica.

Keywords

insecticide resistancelesser grain borerRhyzopertha dominicaesterase activityrésistance aux insecticidesforeur de grainRhyzopertha dominicaactivité estérasique
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 20 , Issue 3 , September 2000 , pp. 207 - 213
Copyright
Copyright © ICIPE 2000

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