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Variation in the coding and non-coding sequences of the DH-PBAN gene of diapause and non-diapause silkworm races

Published online by Cambridge University Press:  01 June 2008

R. Saravanakumar ,
K.M. Ponnuvel  and
C.K. Kamble
R. Saravanakumar
Affiliation:
Biotechnology Laboratory, Central Sericultural Germplasm Resources Centre, PO Box 44, Thally Road, Hosur635 109, Tamil Nadu, India
K.M. Ponnuvel*
Affiliation:
Biotechnology Laboratory, Central Sericultural Germplasm Resources Centre, PO Box 44, Thally Road, Hosur635 109, Tamil Nadu, India
C.K. Kamble
Affiliation:
Biotechnology Laboratory, Central Sericultural Germplasm Resources Centre, PO Box 44, Thally Road, Hosur635 109, Tamil Nadu, India
*
*E-mail: kmpvel@yahoo.com
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Abstract

Diapause hormone (DH) and pheromone biosynthesis-activating neuropeptide (PBAN) code for the diapause gene (DH-PBAN) of Bombyx mori Linnaeus. The DH-PBAN gene sequence from the BLAST database was searched against B. mori genomic gene sequences for similarity. Results showed maximum homology with a genomic contig (accession no. D16230). Primers were designed for the intron, exon and promoter regions of the DH-PBAN gene sequence. The corresponding regions were amplified using genomic DNA as template and products of diapause and non-diapause silkworm races were compared. There was no variation in the 425 bp intron and 254 bp exon regions. However, 1.3% nucleotide sequence variation was observed in selected silkworm races. The promoter region showed 4.2% single nucleotide variation in the diapause and non-diapause silkworm races. The promoter region of the diapause gene showed variations in the sequence of the POU (Pit 1, Oct 1 and Unc-86)-binding site of non-diapause and diapause races of B. mori. In phylogenetic analysis, B. mori formed a cluster with other silkworms of the Bombycidae family, whereas the Noctuidae family formed a separate cluster, indicating that the diapause gene is conserved across the insect taxa.

Keywords

diapauseDH-PBANsilkwormmultivoltinebivoltineBombyx mori
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 28 , Issue 2 , June 2008 , pp. 69 - 77
Copyright
Copyright © ICIPE 2008

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