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DNA Fingerprinting to Improve Data Collection Efficiency and Yield in an Open-Field Host-Specificity Test of a Weed Biological Control Candidate

Published online by Cambridge University Press:  20 January 2017

Brian G. Rector ,
Alessio De Biase ,
Massimo Cristofaro ,
Simona Primerano ,
Silvia Belvedere ,
Gloria Antonini  and
Rouhollah Sobhian
Brian G. Rector*
Affiliation:
Exotic and Invasive Weeds Research Unit, USDA–ARS, 920 Valley Road, Reno, NV 89512
Alessio De Biase
Affiliation:
Department of Biology and Biotechnology “Charles Darwin,” University of Rome “La Sapienza,” Viale dell'Università 32, 00185 Rome, Italy
Massimo Cristofaro
Affiliation:
BBCA-ENEA, ENEA C.R. Casaccia–BIOTEC, Via Anguillarese 301, 00123 Rome, Italy
Simona Primerano
Affiliation:
Department of Biology and Biotechnology “Charles Darwin,” University of Rome “La Sapienza,” Viale dell'Università 32, 00185 Rome, Italy
Silvia Belvedere
Affiliation:
Department of Biology and Biotechnology “Charles Darwin,” University of Rome “La Sapienza,” Viale dell'Università 32, 00185 Rome, Italy
Gloria Antonini
Affiliation:
Department of Biology and Biotechnology “Charles Darwin,” University of Rome “La Sapienza,” Viale dell'Università 32, 00185 Rome, Italy
Rouhollah Sobhian
Affiliation:
European Biological Control Laboratory, USDA-ARS, Campus International de Baillarguet, Montpellier, France
*
Corresponding author's E-mail: brian.rector@ars.usda.gov
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Abstract

An open-field test was conducted in southern France to assess the host-specificity of Ceratapion basicorne (Illiger), a candidate for biological control of yellow starthistle. Test plants were infested by naturally occurring populations of C. basicorne but were also exposed to sympatric herbivore species, including other Ceratapion spp. Insects from the test plants were collected directly into tubes of ethanol and were subsequently identified to species according to DNA sequence similarity with morphologically identified reference specimens. This integrated, morphological and molecular identification method was used in an effort to maximize the amount of data gained in the field bioassay and to minimize the number of taxonomist–hours necessary to complete the study. The results obtained showed that the French C. basicorne population only attacked yellow starthistle and cornflower, another known host of C. basicorne. Molecular phylogenetic analysis of the insects collected from all other nonhost plants rejected the possibility that any were C. basicorne.

Keywords

Cornflower, Centaurea cyanus L. CENCYyellow starthistle, Centaurea solstitialis L. CENSOCeratapion basicorne (Illiger)Apionidaebiological controlhost rangeopen-field testsafflower
Type
Research
Information
Invasive Plant Science and Management , Volume 3 , Issue 4 , December 2010 , pp. 429 - 439
Copyright
Copyright © Weed Science Society of America 

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References

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