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Effects of betaine supplementation on rumen fermentation, lactation performance, feed digestibilities and plasma characteristics in dairy cows

Published online by Cambridge University Press:  28 May 2010

C. WANG ,
Q. LIU ,
W. Z. YANG ,
J. WU ,
W. W. ZHANG ,
P. ZHANG ,
K. H. DONG  and
Y. X. HUANG
C. WANG
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China
Q. LIU*
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China
W. Z. YANG
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China Agriculture and Agri-Food Canada, Research Centre, P. O. Box 3000, Lethbridge, AB, Canada
J. WU
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China
W. W. ZHANG
Affiliation:
Shanxi Xinliyuan Biotechnology Co. Ltd, Taiyuan, Shanxi 030006, People's Republic of China
P. ZHANG
Affiliation:
Datong State Dairy Cow Farm, Datong, Shanxi 037004, People's Republic of China
K. H. DONG
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China
Y. X. HUANG
Affiliation:
College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, People's Republic of China
*
*To whom all correspondence should be addressed. Email: liuqiangabc@163.com
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Summary

The objective of the present study was to evaluate the effects of betaine supplementation on rumen fermentation, lactation performance and plasma characteristics in dairy cows. Twenty multiparous Holstein dairy cows (597±11·8 kg body weight (BW), 88±4·5 days in milk (DIM) and average daily milk production of 26·3±0·5 kg/cow) were used in a replicated 4×4 Latin square experiment. The treatments were: control (without betaine), low-betaine (LB), medium-betaine (MB) and high-betaine (HB) with 0, 50, 100 and 150 g supplemental anhydrous betaine/cow/day, respectively. Betaine was hand-mixed into the top one-third of the daily ration at feeding. Experimental periods were 30 days with 15 days of adaptation and 15 days of sampling. Dry matter (DM) intake was not affected with increasing the betaine supplementation. There were linear increases in milk yield and fat-corrected milk yield (corrected to 40 g fat/kg) and a linear and quadratic increase in milk fat concentration with increasing the betaine supplementation, whereas the proportion and yield of milk protein and lactose, and feed efficiency, were not affected. Ruminal pH and ammonia N linearly decreased, whereas total volatile fatty acid (VFA) concentration linearly and quadratically increased with increasing the betaine supplementation. The ratio of acetate to propionate (A:P) linearly increased from 3·06 to 3·53 as betaine supplementation increased. Digestibility of DM linearly increased, whereas digestibilities of organic matter (OM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) in the total tract were quadratically increased with increasing the betaine supplementation. Plasma concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) were lower for betaine supplementation than for control, and were linearly decreased by betaine supplementation. The results indicate that supplementation of mid-lactation dairy cow diets with betaine increased milk yield through increased feed digestion. Betaine supplementation may benefit lactation performance when methionine supply is limiting.

Type
Animals
Information
The Journal of Agricultural Science , Volume 148 , Issue 4 , August 2010 , pp. 487 - 495
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

AOAC (1990). Official Methods of Analysis. 14th edn.Arlington, VA: Association of Official Analytical Chemists.Google Scholar
AOAC (1997). Official Methods of Analysis. 16th edn, 3rd revision.Gaithersburg, MD: Association of Analytical Chemists Int.Google Scholar
Banskalieva, V., Puchala, R., Goetsch, A. L., Luo, J. & Sahlu, T. (2005). Effects of ruminally protected betaine and choline on net flux of nutrients across the portal-drained viscera and liver of meat goat wethers consuming diets differing in protein concentration. Small Ruminant Research 57, 193–202.CrossRefGoogle Scholar
Bock, B. J., Brethour, J. R., Harmoney, K. R. & Goodall, S. R. (2004). Influence of betaine on pasture, finishing, and carcass performance in steers. Professional Animal Scientist 20, 53–57.CrossRefGoogle Scholar
Bonner, S. L., Gaughan, J. B., Al Jassim, R. A. M. & Bryden, W. L. (2008). Dietary betaine and its effect on rumen VFA profiles in sheep. Proceedings of the Australian Society of Animal Production 27, 107.Google Scholar
Canfield, R. W. & Butler, W. R. (1991). Energy balance, first ovulation and the effects of naloxone on LH secretion in early postpartum dairy cows. Journal of Animal Science 69, 740–746.CrossRefGoogle ScholarPubMed
Davidson, S. (2006). Supplementation of rumen-protected forms of methionine, betaine, and choline to early lactation Holstein cows. PhD thesis, North Carolina State University, Raleigh, NC.Google Scholar
Davidson, S., Hopkins, B. A., Odle, J., Brownie, C., Fellner, V. & Whitlow, L. W. (2008). Supplementing limited methionine diets with rumen-protected methionine, betaine, and choline in early lactation Holstein cows. Journal of Dairy Science 91, 1552–1559.CrossRefGoogle ScholarPubMed
Fernández, C., Gallego, L. & Lopez-Bote, C. J. (1998). Effect of betaine on fat content in growing lambs. Animal Feed Science and Technology 73, 329–338.CrossRefGoogle Scholar
Fernández, C., López-Saez, A., Gallego, L. & De La Fuente, J. M. (2000). Effect of source of betaine on growth performance and carcass traits in lambs. Animal Feed Science and Technology 86, 71–82.CrossRefGoogle Scholar
Fernández, C., Sánchez-Seiquer, P., Sánchez, A., Contreras, A. & De La Fuente, J. M. (2004). Influence of betaine on milk yield and composition in primiparous lactating dairy goats. Small Ruminant Research 52, 37–43.CrossRefGoogle Scholar
Fernández-Figares, I., Wray-Cahen, D., Steele, N. C., Campbell, R. G., Hall, D. D., Virtanen, E. & Caperna, T. J. (2002). Effect of dietary betaine on nutrient utilization and partitioning in the young growing feed-restricted pig. Journal of Animal Science 80, 421–428.CrossRefGoogle Scholar
Ferret, A., Plaixats, J., Caja, G., Gasa, J. & Prió, P. (1999). Using markers to estimate apparent dry matter digestibility, faecal output and dry matter intake in dairy ewes fed Italian ryegrass hay or alfalfa hay. Small Ruminant Research 33, 145–152.CrossRefGoogle Scholar
Gralak, M. A., Leśniewska, V., Puchaa, R., Barej, W. & Dymnicki, E. (1998). The effect of betaine and rumen undegradable choline on growth rate and feed efficiency in calves. Journal of Animal and Feed Sciences 7 (Suppl. 1), 229–233.CrossRefGoogle Scholar
Jacobson, D. R., Lindahl, I. L., Mcneill, J. J., Shaw, J. C., Doetsch, R. N. & Davis, R. E. (1957). Feedlot bloat studies. II. Physical factors involved in the etiology of frothy bloat. Journal of Animal Science 16, 515–524.CrossRefGoogle Scholar
Johnson, M. M. & Peters, J. P. (1993). Technical note: an improved method to quantify nonesterified fatty acids in bovine plasma. Journal of Animal Science 71, 753–756.CrossRefGoogle ScholarPubMed
Kettunen, H., Tiihonen, K., Peuranen, S., Saarinen, M. T. & Remus, J. C. (2001). Dietary betaine accumulates in the liver and intestinal tissue and stabilizes the intestinal epithelial structure in healthy and coccidia-infected broiler chicks. Comparative Biochemistry and Physiology – Part A: Molecular and Integrative Physiology 130, 759–769.CrossRefGoogle ScholarPubMed
Kozloski, G. V., Moraes Flores, E. M. & Martins, A. F. (1999). Use of chromium oxide in digestibility studies: variations of the results as a function of the measurement method. Journal of the Science of Food and Agriculture 76, 373–376.3.0.CO;2-Y>CrossRefGoogle Scholar
Liu, Q., Wang, C., Huang, Y. X., Dong, K. H., Yang, W. Z. & Wang, H. (2008). Effects of Lanthanum on rumen fermentation, urinary excretion of purine derivatives and digestibility in steers. Animal Feed Science and Technology 142, 121–132.CrossRefGoogle Scholar
Löest, C. A., Titgemeyer, E. C., Drouillard, J. S., Blasi, D. A. & Bindel, D. J. (2001). Soybean hulls as a primary ingredient in forage-free diets for limit-fed growing cattle. Journal of Animal Science 79, 766–774.CrossRefGoogle ScholarPubMed
Löest, C. A., Titgemeyer, E. C., Drouillard, J. S., Coetzer, C. M., Hunter, R. D., Bindel, D. J. & Lambert, B. D. (2002). Supplemental betaine and peroxide- treated feather meal for finishing cattle. Journal of Animal Science 80, 2234–2240.Google ScholarPubMed
Melaku, S., Peters, K. J. & Tegegne, A. (2004). Microbial nitrogen supply, nitrogen retention and rumen function in Menz sheep supplemented with dried leaves of multipurpose trees, their mixtures or wheat bran. Small Ruminant Research 52, 25–36.CrossRefGoogle Scholar
Mitchell, A. D., Chappell, A. & Knox, K. L. (1979). Metabolism of betaine in the ruminant. Journal of Animal Science 49, 764–774.CrossRefGoogle ScholarPubMed
NRC (2001). Nutrient Requirements of Dairy Cattle. 7th rev. edn.Washington, DC: National Academy of Sciences.Google Scholar
Puchala, R., Banskalieva, V., Goetsch, A. L., Sahlu, T. & Prieto, I. (2000). Effect of ruminally protected choline and betaine on productivity of Angora doelings. In Proceedings of the Seventh International Conference on Goats, 105 pp. Tours, France: INRA.Google Scholar
Puchala, R., Sahlu, T., Herselman, M. J. & Davis, J. J. (1995). Influence of betaine on blood metaboloites of Alpine and Angora kids. Small Ruminant Research 18, 137–143.CrossRefGoogle Scholar
Russell, J. B., O'Connor, J. D., Fox, D. G., Van Soest, P. J. & Sniffen, C. J. (1992). A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of Animal Science 70, 3551–3561.CrossRefGoogle Scholar
SAS Institute (2002). User's Guide: Statistics, Version 9. Cary, NC: SAS Institute, Inc.Google Scholar
Shenkoru, T. (2001). Effect of betaine and choline supplementation on mohair and milk production. Ph.D. thesis. Oklahoma State University, Stillwater, OK.Google Scholar
Trinder, P. (1969). Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry 6, 24–27.CrossRefGoogle Scholar
Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.CrossRefGoogle Scholar
Wiedmeier, R. D., Tanner, B. H., Bair, J. R., Shenton, H. T., Arambel, M. J. & Walters, J. L. (1992). Effects of a new molasses byproduct, concentrated separator byproduct, on nutrient digestibility and ruminal fermentation in cattle. Journal of Animal Science 70, 1936–1940.CrossRefGoogle ScholarPubMed
Williamson, D. H., Mellanby, J. & Krebs, H. A. (1962). Enzymic determination of D(−)-β-hydroxybutyric acid and acetoacetic acid in blood. Biochemical Journal 82, 90–96.CrossRefGoogle ScholarPubMed