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The isolation of plant cell wall preparations with low nitrogen contents

Published online by Cambridge University Press:  27 March 2009

I. M. Morrison
I. M. Morrison
Affiliation:
Harmah Research Institute, Ayr Ka& 5Hl
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Summary

Grasses of widely differing maturity have been extracted by two chemical and two enzymic methods in order to prepare cell wall materials with low nitrogen contents. These methods involved extraction, with neutral detergent or phenol-acetic acid-water (1:1:1), or digestion with the proteolytic enzymes pepsin or pronase. Wide differences were noted between these methods with grasses at different levels of maturity, both in the nitrogen content of the residue and the actual yield of cell wall preparation. The more efficient of these methods for removing nitrogenous materials, namely neutral detergent extraction and digestion with pepsin and pronase, were also examined for their effect on the structural carbohydrate and lignin content of the cell wall. In all respects, a method involving incubation with the enzyme, pronase, gave the most satisfactory cell wall preparation.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 80 , Issue 3 , June 1973 , pp. 407 - 410
Copyright
Copyright © Cambridge University Press 1973

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References

Bagdasaran, M., Matheson, N. A., Synge, R. L. M. & Youngson, M. A. (1964). New procedures for isolating polypeptides and proteins from tissues. Biochem. J. 91, 91–105.Google Scholar
Bailey, R. W. & Jones, D. I. H. (1972). The hydrolysis of cell wall polysaccharides from freeze-dried and oven-dried herbage by rumen and mould carbohydrases. J. Sci. Fd Agric. 23, 609–14.Google Scholar
Decker, R. F. H., Richards, G. N. & Playne, M. J. (1972). Digestion of polysaccharide constituents of tropical pasture herbage in the bovine rumen. Carbohyd. Bes. 22, 173–85.CrossRefGoogle Scholar
Dubois, M., Gillies, K. A., Hamilton, J. K., Rebers, P. S. & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28, 350–6.CrossRefGoogle Scholar
Jones, D. I. H. & Griffith, C. (1962). Normal-acid fibre method for evaluating roughages. Nature, Lond. 193, 882–3.CrossRefGoogle ScholarPubMed
Mora, K. W. (1972). The effect of different extraction procedures on the recovery of cell walls in forage and faeces from cattle and sheep. J. agric. Sci., Camb. 78, 351–3.CrossRefGoogle Scholar
Morrison, I. M. (1972). A semi-micro method for the determination of lignin and its use in predicting the digestibility of forage crops. J. Sci. Fd Agric. 23, 455–63.CrossRefGoogle ScholarPubMed
Norman, A. G. (1935). The composition of crude fibre. J. agric. Sci., Camb. 25, 529–40.CrossRefGoogle Scholar
Porter, P. & Singleton, A. G. (1971). The degradation of lignin and quantitative aspects of ruminant digestion. Br. J. Nutr. 25, 3–14.CrossRefGoogle ScholarPubMed
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. 1. Preparation of fibre residues of low nitrogen content. J. Ass. off. agric. Chem. 46, 825–9.Google Scholar
Waite, R., Johnston, M. J. & Armstrong, D. G. (1964). The evaluation of artificially dried grass as a source of energy for sheep. 1. The effect of stage of maturity on the apparent digestibility of rye-grass, cocksfoot and timothy. J. agric. Sci., Camb. 62, 391–8.CrossRefGoogle Scholar
Weatherburn, M. W. (1967). Phenol-hypochlorite reaction for determination of ammonia. Analyt. Chem. 39, 971–4.CrossRefGoogle Scholar