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Variability of the blood composition of dairy cows in relation to time of day

Published online by Cambridge University Press:  27 March 2009

R. Mansion ,
G. J. Rowlands ,
W. Little  and
K. A. Collis
R. Mansion
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RO16 0NN
G. J. Rowlands
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RO16 0NN
W. Little
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RO16 0NN
K. A. Collis
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RO16 0NN
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Summary

A blood sample was taken at each hourly interval between 07.00 and 16.00 h on five consecutive days from each of two groups of five lactating and two groups of five nonlactating cows. The sampling was organized so that each cow was sampled twice daily and once at each time of day. Samples were analysed for blood glucose and haemoglobin, serum urea, inorganic phosphate, Ca, Mg, Na, K, total protein, albumin, Fe and Cu and plasma β-hydroxybutyrate. The sources of variation in the concentration of each constituent were determined.

Definite patterns of changes in concentrations with time of day were observed for β-hydroxybutyrate, urea, Ca, Na, Mg and Cu. In lactating cows there was a 2½-fold increase in the concentration of β-hydroxybutyrate from the lowest at 07.00 to the highest at 11.00 h. Variation in the concentrations of the other constituents with time of day was a relatively small proportion of the total variance.

It was concluded that diurnal variation in blood composition was unlikely to be a major source of error in interpretation for the usual constituents of a metabolic profile test. However, if β-hydroxybutyrate were to be included great care would be required in selecting a time for sampling.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 96 , Issue 3 , June 1981 , pp. 593 - 598
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Allcroft, W. M. (1933). Diurnal variations in the blood sugar level of the lactating cow. Biochemical Journal 27, 1820–1823.CrossRefGoogle ScholarPubMed
Baird, G. D., Heitzman, R. J., Hibbitt, K. G. & Hunter, G. D. (1974). Bovine ketosis: A review with recommendations for control and treatment. Part 1. British Veterinary Journal 130, 214–220.CrossRefGoogle Scholar
Coggins, C. R. E. & Field, A. C. (1976). Diurnal variations in the chemical composition of plasma from lactating beef cows on three dietary energy intakes. Journal of Agricultural Science, Cambridge 86, 595–602.CrossRefGoogle Scholar
Downie, J. G. (1975). A study of some factors affecting blood components of beef cows. Ph.D. thesis, University of Aberdeen.Google Scholar
Evans, E., Buchanan-Smith, J. C. & Macleod, G. K. (1975). Post-prandial patterns of plasma glucose, insulin and volatile fatty acids in ruminants fed low and high roughage diets. Journal of Animal Science 41, 1474.CrossRefGoogle Scholar
Fisher, L. J., Donnelly, P. J., Hutton, J. B. & Duganzich, D. M. (1975). Relationships between levels of feeding and certain blood metabolites in dairy cows in mid-lactation. Journal of Agricultural Science, Cambridge 84, 29–37.CrossRefGoogle Scholar
Gitelman, H. J. (1967). An improved method for the determination of caloium in biological specimens. Analytical Biochemistry 18, 521–531.CrossRefGoogle Scholar
Henderson, C. R. (1953). Estimation of variance and covariance components. Biometrics 9, 226–252.CrossRefGoogle Scholar
Hove, K. & Blom, A. K. (1973). Plasma insulin and growth hormone in dairy oows: diurnal variation and relation to food intake and plasma sugar and aceto-acetate levels. Ada Endocrinologica 73, 289–303.Google Scholar
Ichida, R. & Nobuoka, M. (1969). Determination of serum copper with atomic absorption spectrophotometry. Clinica Chimica Ada 24, 299–303.Google ScholarPubMed
Jenny, B. F. & Polan, C. E. (1975). Postprandial blood glucose and insulin in cows fed high grain. Journal of Dairy Science 58, 512–514.CrossRefGoogle ScholarPubMed
Kelly, J. M. (1977). Changes in serum beta-hydroxybutyrate concentrations in dairy cows kept under commercial farm conditions. Veterinary Record 101, 499–502.Google ScholarPubMed
Manston, R., Payne, J. M., Rowlands, G. J. & Dew, S. M. (1971). A new multi-phasic screening technique for dairy cows – the Compton metabolic profile test. Proceedings of Technicon International Symposium, Automation in Analytical Chemistry, pp. 218–220.Google Scholar
Manston, R. & Rowlands, G. J. (1972). Analytical variation in metabolio profile testing. Journal of Dairy Research 40, 85–92.CrossRefGoogle Scholar
Payne, J. M., Dew, S. M., Manston, R. & Faulks, M. (1970). The use of a metabolic profile test in dairy herds. Veterinary Record 87, 150–158.CrossRefGoogle ScholarPubMed
Payne, J. M., Rowlands, G. J., Manston, R. & Dew, S. M. (1973). A statistical appraisal of the results of metabolic profile tests on 75 dairy herds. British Veterinary Journal 129, 370–381.CrossRefGoogle ScholarPubMed
Radloff, H. D., Schultz, L. H. & Hoekstra, W. G. (1966). Relationships of plasma free fatty acids to other blood components in ruminants under various physiological conditions. Journal of Dairy Science 49, 179–182.CrossRefGoogle ScholarPubMed
Robinson, R., Roughan, M. E. & Wagstaff, D. F. (1971). Measuring inorganic phosphate without using a reducing agent. Annals of Clinical Biochemistry 8, 168–170.CrossRefGoogle Scholar
Rowlands, G. J. & Pocock, R. M. (1976). Statistical basis of the Compton metabolio profile test. Veterinary Record 98, 333–338.CrossRefGoogle ScholarPubMed
Unshelm, J. & Hagemeister, H. (1971). Individual diurnal and time-of-day variations in the blood constituents of cattle. Journal of Interdisciplinary Cycle Research 2, 283–289.CrossRefGoogle Scholar
Williamson, D. H. & Mellanby, J. (1974). In Methods of Enzymatic Analysis (ed. Bergmeyer, H. U.), second edition, volume 4, pp. 1836–1839. London and New York: Academic Press.CrossRefGoogle Scholar