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Veno-arterial differences of immunoreactive and biologically active luteinizing hormone across the head in the cow

Published online by Cambridge University Press:  27 March 2009

R. J. van Aarde ,
R. B. Heap ,
M. Hamon  and
I. R. Fleet
R. J. van Aarde
Affiliation:
Mammal Research InstituteUniversity of PretoriaPretoria 0002South Africa
R. B. Heap
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT, UK
M. Hamon
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT, UK
I. R. Fleet
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT, UK
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Summary

Immunoreactive (I) and biologically active (B) luteinizing hormone (LH) was measured in jugular vein and carotid arterial plasma of four cows 110–140 min after luteinizing hormone releasing hormone (LHRH) injection. Plasma was separated on Ultragel AcA54 and fractions corresponding to those containing authentic bovine luteinizing hormone (bLH) were assayed. Immunoreactivity in venous or arterial plasma occurred mostly in fractions corresponding in molecular weight to bLH. Low activity was measured in several other fractions. Biological activity was also high in the same fractions from venous plasma, though it was not so pronounced in arterial plasma.

A veno-arterial difference across the head for immunoreactive LH was found in all animals, and there was a significant difference in biologically active LH (mouse Leydig cell assay). The mean B:I value for immunoreactive LH was similar in venous and arterial plasma for three out of four animals, 0·72 and 0·66, respectively. In a fourth cow with a clinical history of infertility, the B:I value was low in both venous and arterial plasma, 0·19 and 0·08, respectively. The results indicate that infertility in this latter instance was associated with LHRH-induced secretion of a compound similar in molecular size and immunoreactivity to LH but deficient in biological potency.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 113 , Issue 1 , August 1989 , pp. 27 - 33
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Buckingham, J. C. & Wilson, C. A. (1985). Peripubertal changes in the nature of LH. Journal of Endocrinology 104, 173–177.CrossRefGoogle ScholarPubMed
Chappel, S. C. (1985). Neuroendocrine regulation of luteinizing hormone and follicle stimulating hormone: a review. Life Sciences 36, 97–103.CrossRefGoogle ScholarPubMed
Dodson, S. E., Abbott, M. P. & Haresign, W. (1988). Comparison of bioassay and radioimmunoassay data for study of changes in the pattern of LH secretion from birth to puberty in the heifer. Journal of Reproduction and Fertility 82, 539–543.CrossRefGoogle ScholarPubMed
Foulds, L. M. & Robertson, D. M. (1983). Electrofocusing fractionation and characterization of pituitary follicle-stimulating hormone from male and female rats. Molecular and Cellular Endocrinology 31, 117–130.CrossRefGoogle ScholarPubMed
Graesslin, D., Leidenberger, F. A., Lichtenberg, V., Glismann, D., Hess, N., Czygan, P. J. & Betterdorf, G. (1976). Existence of big and little forms of luteinizing hormone in human serum. Acta Endocrinologica 83, 466–482.Google ScholarPubMed
Green, E. D. & Baenziger, J. V. (1988 a). Asparagine-linked oligosaccharides on lutropin, follitropin and thyrotropin. I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine and human pituitary glycoprotein hormones. Journal of Biological Chemistry 263, 25–35.CrossRefGoogle ScholarPubMed
Green, E. D. & Baenziger, J. V. (1988 b). Asparagine-linked oligosaccharides on lutropin, follitropin and thyrotropin. II. Distribution of sulfated and sialylated oligosaccharides on bovine, ovine and human pituitary glycoprotein hormones. Journal of Biological Chemistry 263, 36–44.CrossRefGoogle ScholarPubMed
Green, E. D., Boime, I. & Baenziger, J. V. (1986). Differential processing of Asn-linked oligosaccharides on pituitary glycoprotein hormone: implications for biologic function. Molecular and Cellular Biochemistry 72, 81–100.CrossRefGoogle ScholarPubMed
Hartree, A. S., Lester, J. B. & Shownkeen, R. C. (1985). Studies of the heterogeneity of human pituitary LH by fast protein liquid chromatography. Journal of Endocrinology 105, 405–413.CrossRefGoogle Scholar
Jackson, G. L. & Liu, T. C. (1982). Structure, characteristics, and biosynthesis of prolactin, luteinizing hormone, and follicle-stimulating hormone. In Biochemistry of Mammalian Reproduction (Eds , L. T. D. & Chatterton, R. T.), pp. 309–334. New York: John Wiley & Sons.Google Scholar
Jenkin, G., Heap, R. B. & Symons, D. B. A. (1977). Pituitary responsiveness to synthetic LH–RH and pituitary LH content at various reproductive stages in the sheep. Journal of Reproduction and Fertility 49, 207–214.CrossRefGoogle ScholarPubMed
Keel, B. A. & Grotjan, H. E. Jr, (1984). Characterisation of rat lutropin charge microheterogeneity using chromato-focusing. Analytical Biochemistry 142, 267–270.CrossRefGoogle Scholar
Keel, B. A., Schanbacher, B. D. & Grotjan, H. E. Jr, (1987). Ovine luteinizing hormone. I. Effects of castration and steroid administration on the charge heterogeneity of pituitary luteinizing hormone. Biological Reproduction 36, 1102–1113.CrossRefGoogle ScholarPubMed
Marrama, P., Zaidi, A. A., Montanini, V., Celani, M. F., Cioni, K., Carani, C., Morabito, F., Resentini, M., Bonati, B. & Baraghini, G. F. (1983). Age and sex related variations in biologically active and immunoreactive serum luteinizing hormone. Journal of Endocrinology and Investigation 6, 427–433.CrossRefGoogle ScholarPubMed
Matteri, R. L., Papkoff, H., Ng, D. A., Swerdlow, J. R. & Chang, Y.-S. (1986). Isolation and characterization of three forms of luteinizing hormone from the pituitary gland of the horse. Biological Reproduction 34, 571–578.CrossRefGoogle ScholarPubMed
Meldrum, D. B., Tsao, Z., Monroe, G. D., Braunstein, G. D., Sladek, J., Lu, T. K. H., Vale, W., Rivier, J., Judd, H. L. & Chang, R. J. (1984). Stimulation of LH fragments with reduced bioactivity following GnRH agonist administration in women. Journal of Clinical Endocrinology and Metabolism 58, 755–757.CrossRefGoogle ScholarPubMed
Moss, G. E., Lemenager, R. P., Parfet, J. R., Adams, B. M. & Adams, T. E. (1988). Concentrations of bioactive and immunoactive luteinizing hormone in bovine anterior pituitary tissue. Domestic Animal Endocrinology 5, 185–190.CrossRefGoogle ScholarPubMed
Prentice, L. G. & Ryan, R. J. (1975). LH and its subunits in human pituitary, serum and urine. Journal of Clinical Endocrinology 40, 303–312.CrossRefGoogle ScholarPubMed
Reichert, L. E. Jr, (1971). Electrophoretic properties of pituitary gonadotropins as studied by electrofocusing. Endocrinology 88, 1029–1044.CrossRefGoogle ScholarPubMed
Reiter, E. O., Beitins, I. Z., Ostrea, T. & Gutai, J. P. (1982). Bioassayable luteinizing hormone during childhood and adolescence and in patients with delayed pubertal development. Journal of Clinical Endocrinology and Metabolism 54, 155–161.CrossRefGoogle ScholarPubMed
Robertson, D. M., van Damme, M.-P. & Diczfalusy, E. (1977). Biological and immunological characterization of human luteinizing hormone. 1. Biological profile in pituitary and plasma samples after electrofocusing. Molecular and Cellular Endocrinology 9, 45–56.CrossRefGoogle ScholarPubMed
Sawyer-Steffan, J. E., Lasley, B. C., Hoff, J. D. & Yen, S. S. C. (1982). Comparison of in vitro bioactivity and immunoreactivity of serum LH in normal cyclic and hypogonadal women treated with low doses of LH–RH. Journal of Reproduction and Fertility 65, 45–51.CrossRefGoogle ScholarPubMed
Schumacher, M., Schäfer, G., Holstein, A. F. & Hilz, H. (1978). Rapid isolation of mouse Leydig cells by centrifugation in percoll density gradients with complete retention of morphological and biochemical integrity. FEBS Letters 91, 333–339.CrossRefGoogle ScholarPubMed
Ulloa-Aguirre, A. & Chappel, S. C. (1982). Multiple species of follicle-stimulating hormone exist within the anterior pituitary gland of male golden hamsters. Journal of Endocrinology 95, 257–266.CrossRefGoogle ScholarPubMed
Van Damme, M. P., Robertson, D. M. & Diczfalusy, E. (1974). An improved in vitro bioassay method for measuring luteinizing hormone (LH) activity using mouse Leydig cell preparations. Acta Endocrinologica 77, 655–671.Google ScholarPubMed
Veldhuis, J. D., Johnson, M. L. & Dufau, M. L. (1987). Preferential release of bioactive luteinizing hormone in response to endogenous and low dose exogenous gonadotropin-releasing hormone pulses in man. Journal of Clinical Endocrinology and Metabolism 64, 1275–1282.CrossRefGoogle ScholarPubMed
Ward, D. N., Glenn, S. D., Nahm, H. S. & Wen, T. (1986). Characterization of cleavage products in selected human lutropin preparations. International Journal of Peptide and Protein Research 27, 70–78.CrossRefGoogle ScholarPubMed