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An evaluation of the field performance of the progeny of plants regenerated from embryos of Hordeum vulgare cv. Golden Promise

Published online by Cambridge University Press:  27 March 2009

J. M. Dunwell
Affiliation:
John Innes Institute, Golney Lane, Norwich, NR4 1UH
M. Cornish
Affiliation:
John Innes Institute, Golney Lane, Norwich, NR4 1UH
W. Powell
Affiliation:
Scottish Crop Research Institute, Pentlandfield, Roslin, Midlothian, EH25 9FR, Scotland
E. M. Borrino
Affiliation:
Scottish Crop Research Institute, Pentlandfield, Roslin, Midlothian, EH25 9FR, Scotland

Summary

The study was designed to compare the agronomic performance of progenies of barley plants (cv. Golden Promise) regenerated from immature zygotic embryos (IE) with these originating from embryos produced after chromosome elimination (Hordeum bulbosum technique). Conventionally derived seed material was used as control.

In a randomized complete block design with two replicates, plants were scored for mildew reaction (Erysiphe graminis f.sp. hordei), number of days to awn emergence, height and yield. Data showed no evidence of variation for mildew reaction and although significant differences between lines for the other three characters were observed in the IE-derived sample these were not significantly greater than that observed in the control samples.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Beckert, M., Pollacsek, M. & Caenen, M. (1983). Etude de la variabilité génétique obtenue chez le mais après callogenèse et régénération de plantes invitro. Agronomie 3, 918.CrossRefGoogle Scholar
Deambrogio, E. & Dale, P. J. (1980). Effect of 2,4-D on the frequency of regenerated plants in barley and on genetic variability between them. Cereal Besearch Communications 8, 417423.Google Scholar
Edallo, S., Zucchinali, C, Perenzin, M. & Salamini, F. (1981). Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica 26, 3956.Google Scholar
Evans, D. A., Sharp, W. R. & Medina-Filho, H. P. (1984). Somaclonal and gametoclonal variation. American Journal of Botany 71, 759774.CrossRefGoogle Scholar
Fukui, K. (1983). Sequential occurrence of mutations in a growing rice callus. Theoretical and Applied Genetics 65, 225230.CrossRefGoogle Scholar
Huang, B., Dunwell, J. M., Powell, W., Hayter, A. M. & Wood, W. (1984). The relative efficiency of microspore culture and chromosome elimination as methods of haploid production in Hordeum vulgare L. Zeitschrift fur Pflanzenzuchtung 92, 2229.Google Scholar
Larkin, P. J., Ryan, S. A., Brettell, R. I. S. & Scowcroft, W. R. (1984). Heritable somaclonal variation in wheat. Theoretical and Applied Genetics 67, 443455.CrossRefGoogle ScholarPubMed
Larkin, P. J. & Scowcroft, W. R. (1983). Somaclonal variation and crop improvement. In Genetic Engineering of Plants: an Agricultural Perspective (ed. Kosuge, T., Meredith, C. P. and Hollaender, A.), pp. 289314. New York: Plenum Press.CrossRefGoogle Scholar
Lupotto, E. (1984). Callus induction and plant regeneration from barley mature embryos. Annals of Botany 54, 523529.CrossRefGoogle Scholar
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15, 473497.CrossRefGoogle Scholar
Oono, K. (1985). Putative homozygous mutations in regenerated plants of rice. Molecular and General Genetics 198, 377384.CrossRefGoogle Scholar
Orton, T. J. (1980). Chromosomal variability in tissue cultures and regenerated plants of Hordeum. Theoretical and Applied Genetics 56, 101112.CrossRefGoogle ScholarPubMed
Powell, W., Hayter, A. M., Wood, W., Dunwell, J. M. & Huang, B. (1984). Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare cv. Sabarlis. Heredity 52, 1923.CrossRefGoogle Scholar
Sun, Z-X., Zhao, C-Z., Zheng, K-L., Qi, L-F. & Fu, Y-P. (1983). Somaclonal genetics of rice Oryza saliva L. Theoretical and Applied Genetics 67, 6773.Google Scholar