Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-28T20:36:34.406Z Has data issue: false hasContentIssue false
  • English
  • Français

Diurnal variation in the chemical composition of plasma from lactating beef cows on three dietary energy intakes

Published online by Cambridge University Press:  27 March 2009

C. R. E. Coggins  and
A. C. Field
C. R. E. Coggins
Affiliation:
Moredun Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH
A. C. Field
Affiliation:
Moredun Institute, 408 Gilmerton Road, Edinburgh, EH17 7JH
Get access Rights & Permissions [Opens in a new window]

Summary

Twenty-two beef cows, producing on average 5·5 kg 4% fat corrected milk per day, were offered diets of rolled barley and silage such that eight animals were consuming 70%, seven 95% and seven 120% of their estimated energy requirements. Blood samples were taken at intervals during the day and the plasma concentrations of glucose, free fatty acids (FFA), ketone bodies, total protein, albumin, globulin, urea nitrogen, calcium, inorganic phosphate, magnesium and copper determined.

Diurnal variation was found in the plasma concentration of glucose, FFA, ketone bodies, urea nitrogen, albumin, calcium and magnesium; it was particularly marked for the first four constituents and appeared to be related to feeding.

There were no overall significant differences between groups in the mean daily concentrations of the measures of energy status (glucose, FFA, ketone bodies), but significant (P < 0·001) prefeeding group differences were found in glucose and ketone body concentrations. No significant differences between groups were found for the remaining constituents.

There was a postprandial depression in plasma glucose concentration, which was positively correlated with FFA concentration.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 86 , Issue 3 , June 1976 , pp. 595 - 602
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agricultural Research Council (1965). The nutrient requirements of farm livestock. No. 2. Ruminants. London: Agricultural Research Council.Google Scholar
Allcroft, W. M. (1933). Diurnal variations in the blood-sugar level of the laotating cow. Biochemical Journal 27, 1820–3.CrossRefGoogle Scholar
Annison, E. F. (1960). Plasma non-esterified fatty acids in sheep. Australian Journal of Agricultural Research 11, 58–64.CrossRefGoogle Scholar
Bowden, D. M. (1971). Non-esterified fatty acids and ketone bodies in blood as indicators of nutritional status in ruminants: a review. Canadian Journal of Animal Science 51, 1–13.CrossRefGoogle Scholar
Dole, V. P. (1956). A relation between non-esterified fatty acids in plasma and the metabolism of glucose. Journal of Clinical Investigation 35, 150–4.CrossRefGoogle ScholarPubMed
Duncombe, W. G. (1963). The colorimetric microdetermination of long-chain fatty acids Biochemical Journal 88, 7–10.CrossRefGoogle ScholarPubMed
Economides, S. J., Miller, T. B., Topps, J. H., Gelman, A. L. & Keith, D. G. (1973). A preliminary study of the milk production, bodyweight changes and some blood characteristics of underfed beef cows. British Veterinary Journal 129, 63–72.CrossRefGoogle ScholarPubMed
Erfle, J. D., Fisher, L. J. & Sauer, F. D. (1974). Interrelationships between blood metabolites and an evaluation of their use as criteria of energy status of cows in early lactation. Canadian Journal of Animal Science 54, 293–303.CrossRefGoogle Scholar
Fiske, C. M. & Subbarow, Y. (1925). The colorimetric determination of phosphorus. Journal of Biological Chemistry 66, 375–400.CrossRefGoogle Scholar
Gartner, R. J. W., Ryley, J. W. & Beattie, A. W. (1966). Values and variations of blood constituents in grazing Hereford cattle. Research in Veterinary Science 7, 424–34.CrossRefGoogle ScholarPubMed
Gitelman, H. J. (1967). An improved automated procedure for the determination of calcium in biological specimens. Analytical Biochemistry 18, 521–31.CrossRefGoogle Scholar
Hagemeister, H. & Unshelm, J. (1970). Individuelle, tages-und tageszeitabhangige Schwankungen von Blutbestandteilen beim Rind. 8. Mitteilung: Das Verhalten der Milchsaure (Laktat), der Brenztraubensaure (Pyruvat), des Hamstoffs und des Blutzuckers. Zentralblatt fur Veterinarmedizin, A 17, 13–26.CrossRefGoogle Scholar
Harvey, W. R. (1968). Least-squares analysis of data with unequal sub-class numbers. U.S. Department of Agriculture ARS/20/8.Google Scholar
Hove, K. & Blom, A. K. (1973). Plasma insulin and growth hormone in dairy cows; diurnal variation and relation to food intake and plasma sugar and acetoacetate levels. Ada Endocrinologica 73, 289–303.Google ScholarPubMed
Kronfeld, D. S. (1972). Ketosis in pregnant sheep and laotating cows: a review. Australian Veterinary Journal 48, 680–7.CrossRefGoogle Scholar
Marsh, W. H., Fingerhut, B. & Miller, H. (1965). Automated and manual direct methods for the determination of blood urea. Clinical Chemistry 11, 624–7.CrossRefGoogle ScholarPubMed
Ness, A. T., Dickerson, H. C. & Pastewka, J. V. (1965). The determination of human serum albumin by its specific binding of the anionic dye 2-(4-hydroxybenzeneazo)-benzoic acid. Clinica Chimica Ada 12, 533–41.Google ScholarPubMed
Payne, J. M., Dew, S. M., Manston, R. & Faulks, M. (1970). The use of a metabolic profile test in dairy herds. Veterinary Record 87, 150–8.CrossRefGoogle ScholarPubMed
Radloff, H. D., Schultz, L. H. & Hoekstra, W. G. (1966). Relationships of plasma free fatty acids to other blood components in ruminants under various physiological conditions. Journal of Dairy Science 49, 179–82.CrossRefGoogle ScholarPubMed
Reid, R. L. (1960). The determination of ketone bodies in blood. Analyst, London 85, 265–71.CrossRefGoogle Scholar
Reid, R. L. & Hinks, N. T. (1962). Studies on the carbohydrate metabolism of sheep. XIX. The metabolism of glucose, free fatty acids and ketones after feeding and during fasting or undernourishment of non-pregnant, pregnant and lactating ewes. Australian Journal of Agricultural Research 13, 1124–36.CrossRefGoogle Scholar
Russel, A. J. F., Doney, J. M. & Reid, R. L. (1967). The use of biochemical parameters in controlling nutritional state in pregnant ewes, and the effect of undernourishment during pregnancy on lamb birthweight. Journal of Agricultural Science, Cambridge 68, 351–8.CrossRefGoogle Scholar
Summers, R. M. (1960). Microdetermination of serum copper. Analytical Chemistry 32, 1903–4.CrossRefGoogle Scholar
Sykes, A. R., & Field, A. C. (1973). Effects of dietary deficiencies of energy, protein and calcium on the pregnant ewe. IV. Serum total protein, albumin, globulin, transferrin and plasma urea levels. Journal of Agricultural Science, Cambridge 80, 29–36.CrossRefGoogle Scholar
Thornton, R. F. (1970). Factors affecting the urinary excretion of urea nitrogen in cattle. ii. The plasma urea nitrogen concentration. Australian Journal of Agricultural Research 21, 145–52.CrossRefGoogle Scholar
Trinder, P. (1969). Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry 6, 24–7.CrossRefGoogle Scholar
Unshelm, J. (1969). Individuelle, tages-und tageszeitabhangige Schwankungen von Blutbestandteilen beim Rind. 6. Mitteilung: Das Verhalten der Gesamteiweibkonzentration und ihrer Komponenten. Zentralblatt fur Veterinarmedizin, A 16, 808–19.CrossRefGoogle Scholar
Unshelm, J. & Rappen, W. H. (1968). Individuelle, tages-und tageszeitabhangige Sohwankungen von Blutbestandteilen beim Rind. 1. Mitteilung: Das Verhalten der Mineralstoffe Natrium, Kalium, Kalzium, Magnesium und anorganischer Phosphor. Zentralblatt fur Veterinarmedizin, A 15, 418–37.CrossRefGoogle Scholar
Weichselbaum, T. E. (1946). An aoourate and rapid method for the determination of protein in small amounts of blood serum or plasma. American Journal of Clinical Pathology (Technical Supplement) 10, 40–9.CrossRefGoogle ScholarPubMed