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Diurnal variations in number of ciliate protozoa in the rumen of sheep fed once and twice daily

Published online by Cambridge University Press:  27 March 2009

T. Michalowski  and
P. Muszyński
T. Michalowski
Affiliation:
Department of Animal Physiology, Zoological Institute, Warsaw University, Zwirki i Wigury 93, 02–089 Warszawa, Poland
P. Muszyński
Affiliation:
Department of Animal Physiology, Zoological Institute, Warsaw University, Zwirki i Wigury 93, 02–089 Warszawa, Poland
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Summary

Diurnal changes in number of protozoa were examined in two wethers fed once and twice daily with 0·4 kg of concentrate and 0·4 kg of hay per day. The number of protozoa varied from 4·8 × 105 to 12·5 × 105 cells/ml of rumen fluid, depending on the feeding frequency and time of the day. Entodinia formed 96·3%, Holotricha 2·0%, Diplodinia 1·3%, and Ophryoscolex 0·4% of the total counts. All taxonomic groups of protozoa reached maximum concentration immediately before feeding and decreased by about 60·80% during 4·12 h after feeding. Larger decreases occurred in animals fed once daily.

Rumen fluid volume varied from 3·12 to 4·47 l but in different ways due to feeding frequency. Diurnal patterns of dilution rate of rumen fluid were the same in animals fed once and twice daily, showing the decrease from 0·98 1/h at 09·00 h to 0·311/h at 01·00 h. It is suggested that some factors other than dilution rate may be responsible for the decrease of protozoa after feeding.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 90 , Issue 1 , February 1978 , pp. 1 - 5
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Bailey, N. T. J. (1959). Statistical methods in biology. London: The English Universities Press.Google Scholar
Bauchop, T. & Clarke, R. T. J. (1976). Attachment of ciliate Epidiniwm Crawley to plant fragments in the sheep rumen. Applied and Environmental Microbiology 32, 417–22.Google Scholar
Dogiel, V. A. (1927). Monographie der Familie Ophryoscolecidae. Archiv fur Protistenkunde 59, 1–288.Google Scholar
Hyden, S. (1956). A turbidimetric method for determination of higher polyethylene glycols in biological materials. Kungl. Lantbrukshogskolans Annaler 22, 139–45.Google Scholar
Hyden, S. (1961). The use of reference substances and the measurement of flow in the alimentary tract. In Digestive Physiology and Nutrition of the Ruminant (ed. Lewis, D.), pp. 37–47. London: Butterworths.Google Scholar
Michałowski, T. (1977). Diurnal changes in concentration of rumen ciliates and in occurrence of dividing forms in water buffalo (Bubalus bubalus) fed once daily. Applied and Environmental Microbiology 33, 802–4.CrossRefGoogle ScholarPubMed
Moir, R. S. & Somers, M. (1956). A factor influencing the protozoal population in sheep. Nature, London 178, 1472.Google Scholar
Potter, E. L. & Dehority, B. A. (1973). Effects of change in feed level, starvation, and level of food after starvation upon the concentration of rumen protozoa in the ovine. Applied Microbiology 26, 692–8.CrossRefGoogle ScholarPubMed
Senaud, J., Jouany, J-P., Grain, J. & P., De Puytorac, (1973). Dynamique d'une population de Polyplastron multivesiculatum (Cilié oligotriche) en équilibre dans le rumen de mouton. Comptes Rendus de I'Academie des Sciences de Paris 277, 197–200.Google Scholar
Warner, A. C.I. (1966 a). Diurnal changes in the concentrations of micro-organisms in the rumens of sheep fed limited diets once daily. Journal of General Microbiology 45, 213–35.Google Scholar
Warner, A. C. I. (1966 b). Periodic changes in the concentrations of micro-organisms in the rumen of a sheep fed a limited ration every three hours. Journal of General Microbiology 45, 237–41.CrossRefGoogle ScholarPubMed
Warner, A. C. I. (1966c). Diurnal changes in the concentrations of micro-organisms in the rumens of sheep fed to appetite in pens or at pasture. Journal of General Microbiology 45, 243–51.Google Scholar
Weller, R. A. & Pilgrim, A. F. (1974). Passage of protozoa and volatile fatty acids from the rumen of the sheep and from a continuous in vitro fermentation system. British Journal of Nutrition 32, 341–51.CrossRefGoogle ScholarPubMed