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Effect of soya-bean protein on the ability of gnotobiotic pig intestine to digest and absorb nutrients

Published online by Cambridge University Press:  27 March 2009

B. Ratcliffe ,
M. W. Smith ,
B. G. Miller ,
P. S. James  and
F. J. Bourne
B. Ratcliffe
Affiliation:
Department of Applied Chemistry and Life Sciences, The Polytechnic of North London, Holloway Road, London N7 8DB
M. W. Smith
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT
B. G. Miller
Affiliation:
Department of Veterinary Medicine, University of Bristol, Langford, Bristol BS18 7DU
P. S. James
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT
F. J. Bourne
Affiliation:
Department of Veterinary Medicine, University of Bristol, Langford, Bristol BS18 7DU
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Summary

Gnotobiotic pigs were fed control and soya-bean protein containing diets in an attempt to identify direct effects of this protein on intestinal structure and function.

Feeding diets containing soya-bean protein for 4 days to 21-day-old gnotobiotic pigs increased crypt depth in the lower half of the small intestine. This increase was accompanied by a corresponding reduction in lactase activity, brought about mainly by a decrease in the apparent rate at which this enzyme appeared in the luminal membrane of developing enterocytes. Sucrase and maltase II and III activities increased slightly in tissue taken from pigs fed soya-bean protein. Alanine transport measured in the presence and absence of Na remained unaffected by change of diet.

The structure and function of control gnotobiotic pig intestine differed from that found in normal unweaned piglets. The possible source of these differences and the probable role played by enteric microflora in amplifying initial effects of soya-bean protein on intestinal function is discussed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 112 , Issue 1 , February 1989 , pp. 123 - 130
Copyright
Copyright © Cambridge University Press 1989

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References

Altmann, G. G. & Leblond, C. P. (1970). Factors influencing villus size in the small intestine of adult rats as revealed by transposition of intestinal segments. American Journal of Anatomy 127, 15–36.CrossRefGoogle ScholarPubMed
Anderson, J. C. (1977). The response of gut-associated lymphoid tissue in gnotobiotic piglets to the presence of bacterial antigen in the alimentary tract. Journal of Anatomy 124, 555–562.Google Scholar
Coates, M. E. & Fuller, R. (1977). The gnotobiotic animal in the study of gut microbiology. In Microbiol Ecology of the Gut (ed. Clarke, R. T. J. and Bauchop, T.), pp. 311–346. London: Academic Press.Google Scholar
Dahlqvist, A. (1960). Characterisation of three different hog intestinal maltases. Acta Chemica Scandinavica 13, 1659–1667.CrossRefGoogle Scholar
Dowling, R. H. (1982). Small bowel adaptation and its regulation. Scandinavian Journal of Gastroenterology supplement 74, 53–74.Google ScholarPubMed
Dziaba, K. A., Lambrecht, G. & Petzoldt, K. (1985). Intestinal and serum antibody response in gnotobiotic piglets to oral immunization with Escherichia coli. Comparative Immunology, Microbiology and Infectious Diseases 8, 267–272.CrossRefGoogle ScholarPubMed
Hampson, D. J. (1986). Alterations in piglet small intestinal structure at weaning. Research in Veterinary Science 40, 32–40.CrossRefGoogle ScholarPubMed
Hampson, D. J. & Kidder, D. E. (1986). Influence of creep feeding and weaning on brush border enzyme activities in the piglet small intestine. Research in Veterinary Science 40, 24–31.CrossRefGoogle ScholarPubMed
Hoerlein, A. B. (1956). The influence of colostrum on antibody response in baby pigs. Journal of Immunology 78, 112–117.CrossRefGoogle Scholar
Lojda, Z. & Kraml, J. (1971). Indogenic methods for glycosidases. III. Ant improved method with 4-Cl-5-Brindolyl-β-D-fucoside and its application in studies of enzymes in the intestine, kidney and other tissues. Histochemie 25, 195–207.CrossRefGoogle Scholar
Lund, E. K., Bruce, M. G., Smith, M. W. & Ferguson, A. (1986). Selective effects of graft-versus-host reaction on disaccharidase expression by mouse jejunal enterocytes. Clinical Science 71, 189–198.CrossRefGoogle ScholarPubMed
Manners, M. J. & Stevens, J. A. (1972). Changes from birth to maturity in the pattern of distribution of lactase and sucrase activity in the mucosa of the small intestine of pigs. British Journal of Nutrition 28, 113–127.CrossRefGoogle ScholarPubMed
Markwell, M. A. K., Haas, A. M., Bieber, L. L. & Tolbert, N. E. (1978). Modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87, 206–210.CrossRefGoogle ScholarPubMed
McCarthy, D. M., Nicholson, J. A. & Kim, Y. S. (1980). Intestinal enzyme adaptation to normal diets of different composition. American Journal of Physiology, 239, G445–G451.Google ScholarPubMed
Miller, B. G., James, P. S., Smith, M. W. & Bourne, F. J. (1986). Effect of weaning on the capacity of pig intestinal villi to digest and absorb nutrients. Journal of Agricultural Science, Cambridge 107, 579–589.CrossRefGoogle Scholar
Miller, B. G., Newby, T. J., Stokes, C. R. & Bourne, F. J. (1984). Influence of diet on postweaning malabsorption and diarrhoea in the pig. Research in Veterinary Science 36, 187–193.CrossRefGoogle ScholarPubMed
Mowat, A. M. & Ferguson, A. (1981). Hypersensitivity reactions in the small intestine. 6. Pathogenesis of the graft-versus-host reaction in the small intestinal mucosa of the mouse. Transplantation 32, 238–243.CrossRefGoogle ScholarPubMed
Pabst, R., Geist, M., Rothkötter, H. J. & Fritz, F. J. (1988). Postnatal development and lymphocyte production of jejunal and ileal Peyer's patches in normal and gnotobiotic pigs. Immunology 64, 539–544.Google ScholarPubMed
Phillips, A. D., Smith, M. W. & Walker-Smith, J. A. (1988). Selective alteration of brush-border hydrolases in intestinal diseases in childhood. Clinical Science 74, 193–200.CrossRefGoogle ScholarPubMed
Ratcliffe, B. & Fordham, J. P. (1987). A technique for rearing germfree piglets obtained without surgery. Laboratory Animals 21, 53–59.CrossRefGoogle ScholarPubMed
Ratcliffe, B., Smith, M. W., Miller, B. G. & Bourne, F. J. (1987). The effect of soya-bean protein on intestinal morphology, absorption and enzyme activity in gnotobiotic pigs at 3 weeks of age. Proceedings of the Nutrition Society 46, 101A.Google Scholar
Smith, M. W. (1985). Expression of digestive and absorptive function in differentiating enterocytes. Annual Review of Physiology 47, 247–260.CrossRefGoogle ScholarPubMed
Smith, M. W., Miller, B. G., James, P. S. & Bourne, F. J. (1985). Effect of weaning on the structure and function of piglet small intestine. In Digestive Physiology in the Pig (ed. Just, A., Jorgensen, H. and Fernandez, J. A.), pp. 75–78. Report No. 580 from the National Institute of Animal Science, Denmark.Google Scholar
Tlaskalova-Hogenova, H., Cerna, J. & Mandel, L. (1981). Peroral immunization of germfree piglets: appearance of antibody-forming cells and antibodies of different isotypes. Scandinavian Journal of Immunology 13, 467–472.CrossRefGoogle ScholarPubMed
Tzipori, S., Robins-Browne, R. M., Gonis, G., Hayes, J., Withers, M. & McCartney, E. (1985). Enteropathogenic Escherichia coli enteritis: evaluation of the gnotobiotic piglet as a model of human infection. Gut 26, 570–578.CrossRefGoogle Scholar