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Germination vigour difference of superior and inferior rice grains revealed by physiological and gene expression studies

Published online by Cambridge University Press:  10 July 2018

Y. F. Zhao ,
H. Z. Sun ,
H. L. Wen ,
Y. X. Du ,
J. Zhang ,
J. Z. Li ,
T. Peng  and
Q. Z. Zhao
Y. F. Zhao
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
H. Z. Sun
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
H. L. Wen
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
Y. X. Du
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
J. Zhang
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
J. Z. Li
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
T. Peng*
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
Q. Z. Zhao*
Affiliation:
Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, China Research Center for Rice Engineering in Henan Province, Henan Agricultural University, Zhengzhou 450002, China Key Laboratory of Rice Biology in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
*
Author for correspondence: T. Peng, E-mail: lypengting@163.com Q. Z. Zhao, E-mail: qzzhaoh@126.com
Author for correspondence: T. Peng, E-mail: lypengting@163.com Q. Z. Zhao, E-mail: qzzhaoh@126.com
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Abstract

Superior and inferior rice grains have different weights and are located on the upper primary branch and lower secondary branches of the panicle, respectively. To study differences in germination vigour of these two types of grain, a number of factors were investigated from 0 to 48 h of germination. The present study demonstrated that in inferior grains the starch granule structure was looser at 0 h, with full water absorption at 48 h, while in superior grains the structure was tight and dense. Relative water content increased, and dry matter decreased, more rapidly in inferior grains than in superior ones. Abscisic acid and gibberellin levels, as well as α-amylase activity, also changed more rapidly in inferior grains, while soluble sugar content and amylase coding gene expression increased more rapidly in inferior than superior grains during early germination. The expression of OsGAMYB was higher in inferior grains at 24 h but higher in superior grains at 48 h. The phenotypic index of seedlings was higher in seedlings from superior grains at the two-leaf stage. However, the thousand-grain weight and yield per plant in superior and inferior plants showed no significant difference at harvest. The present study indicates that inferior grains germinate faster than superior ones in the early germination stage. Although inferior grains produced weaker seedlings, it is worthwhile using them in rice production due to their comparative yield potential over that of superior grains.

Keywords

Ricegermination vigourinferior grainssuperior grains
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 3 , April 2018 , pp. 350 - 357
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Copyright
Copyright © Cambridge University Press 2018 

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