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The metabolism of glucose, acetate, lipids and amino acids in lactating dairy cows

Published online by Cambridge University Press:  27 March 2009

R. Bickerstaffe ,
E. F. Annison  and
J. L. Linzell
R. Bickerstaffe
Affiliation:
Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford
E. F. Annison
Affiliation:
Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford
J. L. Linzell
Affiliation:
A.R.C.Institute of Animal Physiology, Babraham, Cambridge
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Summary

Specialized techniques, previously used in surgically prepared goats, which simultaneously measure udder metabolism (arteriovenous difference of milk precursors x udder blood flow) and the whole body turnover of the milk precursors, have been successfully transferred to dairy cows. Methods of obtaining representative samples of arterial and mammary venous blood and of measuring udder blood flow are described.

The rates of entry into the circulation, as determined by isotope dilution, of glucose, acetate and plasma free fatty acids were 3·3–4·0, 1·7–2·1 and 0·5 kg/day respectively. Acetate and glucose contributed 32–50 and 4–11% respectively of the total CO2output by the animal.

Measurement of the uptake of precursors of milk constituents and their transfer into milk showed that there were substantial arteriovenous differences of glucose, acetate, triglyceride and β-hydroxybutyrate which were not significantly different between breeds or related to milk yield. Isotopic and balance data confirm that glucose is the main precursor of lactose and that the oxidation and transfer of glucose into lactose accounted for 69–98% of the glucose entry rate. As in the goat, plasma triglycerides and blood acetate accounted for 35–80% and 25–50% of the milk triglycerides respectively. Propionate was extracted from plasma but the uptake was only about 8% of the value for acetate.

There was no net arteriovenous difference of phospholipids, cholesterol esters or free fatty acids, but the fall in specific radioactivity of free fatty acids across the mammary gland indicated there was an exchange of free fatty acids between plasma and mammary tissue. In agreement with previous findings, acetate contributed to all the milk fatty acids up to a chain length of C14 and part of the C16 fatty acid. Plasma triglycerides contributed to the remainder of the C16 fatty acid and all the milk fatty acids with a chain length of C18 or higher.

In contrast to the lactating goat, cow plasma contained very few chylomicrons. The majority of the triglycerides taken up by the udder were derived from the lowdensity lipoprotein fraction.

The essential amino acids were extracted from blood in amounts sufficient to account for the essential amino acids secreted into milk protein. Although the plasma level of methionine was low, 52–72% of the material reaching the mammary gland was taken up. The uptake of arginine was far in excess of the requirement for milk protein synthesis.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 82 , Issue 1 , February 1974 , pp. 71 - 85
Copyright
Copyright © Cambridge University Press 1974

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References

REFERENCES

Annison, E. F. (1971). The mammary metabolism of glucose, acetate and ketone bodies. In Lactation (ed. Falconer, I. R.), pp. 281–95. London: Butterworth.Google Scholar
Annison, E. F., Bickerstaffe, R. & Linzell, J. L. (1974) Glucose and fatty acid metabolism in cows, producing milk of low fat content. Journal of Agricultural Science, Cambridge 82, 87–95.CrossRefGoogle Scholar
Annison, E. F., Brown, R. E., Leng, R. A., Lindsay, D. B. & West, C. E. (1967). Rates of entry and oxidation of acetate, glucose, D( – )-β-hydroxybutyrate, palmitate, oleate and stearate, and rates of production and oxidation of propionate and butyrate in fed and starved sheep. Biochemical Journal 104, 135–47.CrossRefGoogle ScholarPubMed
Annison, E. F. & Linzell, J. L. (1964). The oxidation and utilization of glucose and acetate by the mammary gland of the goat in relation to their overall metabolism and to milk formation. Journal of Physiiology, London 175, 37285.CrossRefGoogle ScholarPubMed
Annison, E. F., Linzell, J. L., Fazakerley, S. & Nichols, B. W. (1967). The oxidation and utilization of palmitate, stearate, oleate and acetate by the mammary gland of the fed goat in relation to their overall metabolism and the role of plasma phospholipids and neutral lipids in milk-fat synthesis. Biochemical Journal 102, 637–47.CrossRefGoogle ScholarPubMed
Annison, E. F., Linzell, J. L. & West, C. E. (1968). Mammary and whole animal metabolism of glucose and fatty acids in fasting lactating goats. Journal of Physiology, London 197, 445–59.CrossRefGoogle ScholarPubMed
Barry, J. M. (1964). Quantitative balance between substrates and metabolic products of the mammary gland. Biological Reviews 39, 194–213.CrossRefGoogle ScholarPubMed
Bickerstaffe, R. (1971). Uptake and metabolism of fat in the lactating mammary gland. In Lactation (ed. Falconer, I. R.), pp. 317–32. London: Butterworth.Google Scholar
Emery, R. S., Brown, L. D. & Bell, J. W. (1965). Correlation of milk fat with dietary and metabolic factors in cows fed restricted-roughage rations supplemented with magnesium oxide or sodium bicarbonate. Journal of Dairy Science 48, 1647–51.CrossRefGoogle Scholar
Freeman, C. P., Noakes, D. E. & Annison, E. F. (1970). The metabolism of glucose, acetate, palmitate, stearate and oleate in pigs. British Journal of Nutrition 24, 705–16.CrossRefGoogle Scholar
Glascock, R. F., Welch, V. A., Bishop, C., Davies, T., Wright, E. W. & Noble, R. C. (1966). An investigation of serum lipoproteins and of their contribution to milk fat in the dairy cow. Biochemical Journal 98, 149–56.CrossRefGoogle ScholarPubMed
Graham, W. R., Kay, H. D. & Mointosh, R. A. (1936). A convenient method for obtaining bovine arterial blood. Proceedings of the Royal Society B 120, 319–29.Google Scholar
Griel, L. C. Jr, & McCarthy, R. D. (1969). Blood serum lipoproteins. A review. Journal of Dairy Science 52, 1233–43.CrossRefGoogle ScholarPubMed
Hanwell, A. & Linzell, J. L. (1972). Determination of cardiac output and mammary blood flow in the conscious lactating rat. Journal of Physiology, London 226, 24–5P.Google ScholarPubMed
Hartmann, P. E. & Lascelles, A. K. (1964). The uptake of plasma lipid and some non-lipid constituents by the mammary gland of the cow. Australian Journal of Biological Science 17, 935–44.CrossRefGoogle Scholar
Hartmann, P. E. & Lascelles, A. K. (1965). The effect of starvation on the uptake of the precursors of milk fat by the bovine mammary gland. Australian Journal of Biological Science 18, 1025–34.CrossRefGoogle ScholarPubMed
Huggett, A. St G. & Nixon, D. A. (1957). Enzymic determination of blood glucose. Biochemical Journal 66, 12p.Google Scholar
Junge, F. (1963). Volumenmessung am Euter der Ziege und ihre Beziehungen zur Milchleistung. Inaug. Diss. Tierartzl. Fak. Ludwig. Maximilians Univ., München.Google Scholar
Kaufmann, M. & Magne, H. (1906). Sur la consommation du glucose du sang par le tissue de la glande mammaire. Compte rendu hebdomadaires des Seances de I'Acadamie des Sciences, Paris 143, 779–82.Google Scholar
Ketty, S. S. & Schmidt, J. H. (1945). Determination of cerebral blood flow in man by use of nitrous oxide in low concentrations. American Journal of Physiology 143, 53–66.CrossRefGoogle Scholar
Kronfeld, D. S. (1965). Plasma non-esterified fatty acid concentration in the dairy cow; responses to nutritional and hormonal stimuli and significance in ketosis. Veterinary Record 77, 30–5.Google Scholar
Kronfeld, D. S., Raggi, F. & Ramberg, C. F. (1968). Mammary blood flow and ketone body metabolism in normal, fasted and ketotic cows. American Journal of Physiology 215, 218–27.CrossRefGoogle ScholarPubMed
Lattrell, S. (1957). Turnover rate of unesterified fatty acids in human plasma. Acta physiologica scandinavica 41, 158–67.Google Scholar
Linzell, J. L. (1960 a). Valvular incompetence in the venous drainage of the udder. Journal of Physiology, London 153, 481–91.CrossRefGoogle ScholarPubMed
Linzell, J. L. (1960 b). Mammary gland blood flow and oxygen, glucose and volatile fatty acid uptake in the conscious goat. Journal of Physiology, London 153, 492–509.CrossRefGoogle ScholarPubMed
Linzell, J. L. (1963). Carotid loops. American Journal Veterinary Research 24, 223–4.Google Scholar
Linzell, J. L. (1966 a). Measurement of udder volume in live goats as an index of mammary growth and function. Journal of Dairy Science 49, 307–11.CrossRefGoogle Scholar
Linzell, J. L. (1966 b). Measurement of venous flow by continuous thermodilution and its application to measurement of mammary blood flow in the goat. Circulation Research 18, 745–54.CrossRefGoogle ScholarPubMed
Linzell, J. L. (1966 c). Infusion and blood sampling techniques for use in minimally restrained goats. Journal of Physiology, London 186, 79P.Google ScholarPubMed
Linzell, J. L. (1971). Techniques for measuring nutrient uptake by the mammary glands. In Lactation (ed. Falconer, I. R.), pp. 261–75. London: Butterworth.Google Scholar
Linzell, J. L. (1974). Mammary blood flow and methods of identifying and measuring precursors of milk. In Lactation (ed. Larson, B. L. and Smith, B. R.). New York, London: Academic Press. (In the Press.)Google Scholar
Linzell, J. L., Mepham, T. B., Annison, E. F. & West, C. E. (1969). Mammary metabolism in lactating sows: arteriovenous differences of milk precursors and the mammary metabolism of [14C]glucose and [14C]acetate. British Journal of Nutrition 23, 319–32.CrossRefGoogle Scholar
Mangan, J. L. & Wright, P. C. (1968). The measurement of rumen volumes of sheep and cattle with lithium salts. Research in Veterinary Sciences 9, 366–75.CrossRefGoogle ScholarPubMed
Mepham, T. B. & Linzell, J. L. (1966). A quantitative assessment of the contribution of individual plasma amino acids to the synthesis of milk proteins by the goat mammary gland. Biochemical Journal 101, 76–83.CrossRefGoogle Scholar
Rasmussen, F. (1963). The mammary blood flow in the goat as measured by antipyrine absorption. Acta veterinaria Scandinavica 4, 271–80.CrossRefGoogle Scholar
Rasmussen, F. (1965). The mammary blood flow in the cow as measured by the antipyrine absorption method. Acta veterinaria Scandinavica 6, 135–49.Google ScholarPubMed
Reynolds, M., Linzell, J. L. & Rasmussen, F. (1968). Comparison of four methods for measuring mammary blood flow in conscious goats. American Journal of Physiology 214, 1415–24.CrossRefGoogle ScholarPubMed
Schoefl, G. I. & French, J. E. (1968). Vascular permeability to particulate fat: morphological observations on vessels of lactating mammary gland and of lung. Proceedings of the Royal Society B 169, 163–65.Google Scholar
Seldinger, S. I. (1953). Catheter replacement of the needle in percutaneous arteriography: a new technique. Acta radiologica 39, 368–76.CrossRefGoogle ScholarPubMed
Shaw, J. C. & Petersen, W. E. (1939). Blood volume changes in the mammary gland. Proceedings of the Society of Experimental Biology, N. Y. 42, 520–4.CrossRefGoogle Scholar
Van Leersum, E. C. (1911). Eine Methode zur Erleichterung der Blütdruckmessung bei Tieren. Pflügers Archiv 142, 377–95.CrossRefGoogle Scholar
Verbeke, R. & Peeters, G. (1965). Uptake of free plasma amino acids by the lactating cow's udder and amino acid composition of udder lymph.Biochemical Journal 194, 183–9.CrossRefGoogle Scholar
West, C. E., Bickerstaffe, R., Annison, E. F. & Linzell, J. L. (1972). Studies on the mode of uptake of blood triglycerides by the mammary gland of the lactating goat. Biochemical Journal 126, 477–90.CrossRefGoogle ScholarPubMed
Zierler, K. L. (1961). Theory of the use of arteriovenous concentration differences for measuring metabolism in steady and non-steady states. Journal of Clinical Investigation 40, 2111–25.CrossRefGoogle ScholarPubMed