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Partial Sterilisation of Soil by Volatile and Non-Volatile Antiseptics

Published online by Cambridge University Press:  27 March 2009

Walter Buddin
Affiliation:
(Rolhamsted Experimental Station, Harpenden.)

Extract

1. The characteristics of true partial sterilisation have been found to be common to a large number of antiseptics and are

(a) An initial decrease in the numbers of bacteria followed by a large sustained rise.

(b) The killing of protozoa and nitrifying organisms. In no case have we observed all the usual partial sterilisation phenomena without the death of the larger protozoa which occur abundantly in cultures made from our soils.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1914

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References

page 418 note 1 Arbeiten der Biolog. Abteilung f. Land u. Forstwirtschaft, 1903, Bel. 3, Heft 5.Google Scholar

page 420 note 1 Fowler, Cp., Ardern, and Lockett, , Proc. Roy. Soc. 1910, 83. B., 149.CrossRefGoogle Scholar

page 421 note 1 The numbers of bacteria are probably depressed in the untreated soil immediately after it is spread out, but the treated soils suffer an initial depression before they are spread out for volatilisation of the chemical. The cultural methods used did not demonstrate any action on the protozoa arising from the spreading out.

page 421 note 2 Hutchinson, H. B. and MacLennan, K., Journ. Agr. Sci. 1914, VI. p. 302.CrossRefGoogle Scholar

page 423 note 1 The differences between the various soils used in these experiments was probably not sufficient to give rise to any serious narrowing of these limits.

page 425 note 1 Journ. Agric. Sci. 1910, III. p. 233.Google Scholar

page 428 note 1 Difficulty was experienced at first in obtaining reliable counts of the numbers of bacteria prevailing after treatment owing to the hot weather and to the presence of a large proportion of actively liquefying organisms. The counts given above, however, obtained later in the incubation period, represent accurately the relative numbers prevailing in the various soils.

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page 444 note 2 Russell, E. J. and Petherbridge, F. R., Journ. Agric. Sci. 1913, V. 248CrossRefGoogle Scholar

page 445 note 1 This has been shown to be due probably to the calcium sulphide being washed out of the soil in the extraction and then interfering with the reduction of the zinc-copper couple. 1·2 gms. of calcium sulphide (corresponding to the amount present in 200 gms. of the soil treated with the M/10 dose.) were shaken thoroughly with water, allowed to stand and filtered off. To the filtrate was then added 20 c.c. of a standard nitrate solution and the boiling down with magnesia, acidifying with acetic acid and reduction carried out in the usual way. 20 c.c. of the standard nitrate solution were found to contain 4 m.gms. of N, but tho solution treated with calcium sulphide yielded only 0·7 m.gm. of N as ammonia on distillation.

page 446 note 1 e.g. Chancrin, and Desriot, . J. Agri. Prat. n. ser. 21 (1911), No. 14, pp. 427429Google Scholar. Abst. in E. S. R. XXV. p. 519.

page 446 note 2 Landw. Versuchs. Stationen, Bd. 83. Heft 5 and 6. p. 359Google Scholar.

page 446 note 3 Report of the Woburn Expt. Stn. on Pot Culture Expts., Journ. Roy. Agric. Soc. 1913.

page 446 note 4 Abst. in New Zealand Journ. of Agri. VIII. No. 4, p. 412.Google Scholar

page 446 note 5 This Journal, 1913. V. 173.Google Scholar