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Temperature as a key factor for successful inoculation of soybean with Bradyrhizobium spp. under cool growing conditions in Belgium

Published online by Cambridge University Press:  22 August 2018

J. Pannecoucque ,
S. Goormachtigh ,
J. Ceusters ,
J. Debode ,
C. Van Waes  and
J. Van Waes
J. Pannecoucque*
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Crop Husbandry, Soil and Environment, Burg. Van Gansberghelaan 109, 9820 Merelbeke, Belgium
S. Goormachtigh
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Crop Husbandry, Soil and Environment, Burg. Van Gansberghelaan 109, 9820 Merelbeke, Belgium
J. Ceusters
Affiliation:
KU Leuven, Department of Microbial and Molecular Systems, Faculty of Engineering Technology, Technology Campus Geel, Kleinhoefstraat 4, 2440 Geel, Belgium
J. Debode
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Crop Protection, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium
C. Van Waes
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Crop Husbandry, Soil and Environment, Burg. Van Gansberghelaan 109, 9820 Merelbeke, Belgium
J. Van Waes
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Crop Husbandry, Soil and Environment, Burg. Van Gansberghelaan 109, 9820 Merelbeke, Belgium
*
Author for correspondence: J. Pannecoucque, E-mail: joke.pannecoucque@ilvo.vlaanderen.be
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Abstract

Bacterial inoculation of soybean seeds to improve biological nitrogen fixation is a well-known practice to achieve higher seed and protein yield with reduced fertilization. The optimal inoculation strategy in temperate regions is unknown because soybeans are rarely cultivated under colder growing conditions. The aim of the present work was to determine the most suitable inoculation strategy for soybean cultivation in Belgium. Field trials were set up with four Bradyrhizobium inoculants (HiStick, Force 48, Biodoz and Optimize) at two locations over 2 years (2014–2015) and compared with a non-inoculated control treatment. In addition, HiStick was tested at three doses and Optimize at two time periods prior to sowing. Under Belgian conditions, all inoculants were effective in establishing rhizobial symbiosis, resulting in increased yield, protein content, protein yield and thousand-grain weight compared with the non-inoculated control. A single dose of HiStick was sufficient to establish symbiosis. Pre-inoculation with Optimize 2 weeks before sowing gave an intermediate performance for most parameters between the non-inoculated control treatment and inoculation with Optimize 24 h prior to sowing. Among the four products tested, Biodoz seemed the best product for inoculation under cool growing conditions. Based on the atpD gene, the bacterial strain of Biodoz showed complete similarity with Bradyrhizobium diazoefficiens, while strains of other products were identified as Bradyrhizobium japonicum. In vitro growing capacity of the Biodoz strain at 8 °C was higher compared with the other strains. Better cold adaptation of the Biodoz strain might be a possible explanation for the better performance of Biodoz in Belgium.

Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 4 , May 2018 , pp. 493 - 503
Copyright
Copyright © Cambridge University Press 2018 

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References

Albareda, M, Rodriguez-Navarro, DN, Camacho, M and Temprano, FJ (2008) Alternatives to peat as a carrier for rhizobia inoculants: solid and liquid formulations. Soil Biology and Biochemistry 40, 2771–2779.Google Scholar
Albareda, M, Rodriguez-Navarro, DN and Temprano, FJ (2009) Soybean inoculation: dose, N fertilizer supplementation and rhizobia persistence in soil. Field Crops Research 113, 352–356.Google Scholar
Aper, J, De Clercq, H and Baert, J (2016) Agronomic characteristics of early-maturing soybean and implications for breeding in Belgium. Plant Genetic Resources 14, 142–148.Google Scholar
Bates, D, Mächler, M, Bolker, BM and Walker, SC (2015) Fitting linear mixed-effects models using lme4. Journal of Statistical Software 67, 1–48.Google Scholar
Broughton, WJ, Zhang, F, Perret, X and Staehelin, C (2003) Signals exchanged between legumes and Rhizobium: agricultural uses and perspectives. Plant and Soil 252, 129–137.Google Scholar
Catroux, G, Hartmann, A and Revellin, C (2001) Trends in rhizobial inoculant production and use. Plant and Soil 230, 21–30.Google Scholar
Chueire, LMDO and Hungria, M (1997) N2-fixation ability of Brazilian soybean cultivars with Sinorhizobium fredii and Sinorhizobium xinjiangensis. Plant and Soil 196, 1–5.Google Scholar
Coenye, T, Falsen, E, Vancanneyt, M, Hoste, B, Govan, JR, Kersters, K and Vandamme, PAR (1999) Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. International Journal of Systematic Bacteriology 49, 405–413.Google Scholar
Deaker, R, Roughley, RJ and Kennedy, IR (2004) Legume seed inoculation technology – a review. Soil Biology & Biochemistry 36, 1275–1288.Google Scholar
Delamuta, JRM, Ribeiro, RA, Ormeño-Orrillo, E, Melo, IS, Martinez-Romero, E and Hungria, M (2013) Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. International Journal of Systematic and Evolutionary Microbiology 63, 3342–3351.Google Scholar
Duzan, HM, Mabood, F, Souleimanov, A and Smith, DL (2006) Nod Bj-V (C18:1, MeFuc) production by Bradyrhizobium japonicum (USDA110, 532C) at suboptimal growth temperatures. Journal of Plant Physiology 163, 107–111.Google Scholar
Edwards, U, Rogall, T, Blöcker, H, Emde, M and Böttger, E (1989) Isolation and direct complete nucleotide determination of entire genes – characterization of a gene coding for 16S-ribosomal RNA. Nucleic Acids Research 17, 7843–7853.Google Scholar
Egnér, H, Riehm, H and Domingo, WR (1960) Untersuchungen über die chemische Bodenanalyse als Grundlage für die Beurtleilung des Nährstoffzustandes der Böden. II. Chemische Extraktionsmethoden zur Phosphor- und Kaliumbestimmung. Kungliga Lantbrukshögskolans 26, 199–215.Google Scholar
Herridge, D, Gemell, G and Hartley, E (2002) Legume inoculants and quality control. In Herridge, D (ed.), Inoculants and Nitrogen Fixation of Legumes in Vietnam, ACIAR Proceedings 109e. Canberra, Australia: Australian Centre for International Agricultural Research, pp. 105–115.Google Scholar
Herridge, DF, Hartley, E and Gemell, LG (2014) Rhizobial counts in peat inoculants vary amongst legume inoculant groups at manufacture and with storage: implications for quality standards. Plant and Soil 380, 327–336.Google Scholar
IndexMundi (2017) European Union (EU-27) Soybean Meal Imports by Year. IndexMundi. Available online from: https://www.indexmundi.com/agriculture/?country=eu&commodity=soybean-meal&graph=imports (Accessed 13 June 2018).Google Scholar
KMI (2017) Maandelijkse Normalen. Brussels, Belgium: KMI. Available online from: http://www.meteo.be/meteo/view/nl/360955-Maandelijkse+normalen.html#ppt_16230336 (Accessed 13 June 2018).Google Scholar
Miransari, M and Smith, DL (2007) Overcoming the stressful effects of salinity and acidity on soybean nodulation and yields using signal molecule genistein under field conditions. Journal of Plant Nutrition 30, 1967–1992.Google Scholar
Miransari, M, Riahi, H, Eftekhar, F, Minaie, A and Smith, DL (2013) Improving soybean (Glycine max L.) N2 fixation under stress. Journal of Plant Growth Regulation 32, 909–921.Google Scholar
Monsanto (2015) Optimize ST Soybean Inoculant. Winnipeg, Canada: Monsanto. Available online from: http://www.monsantobioag.com/global/ca/Products/Pages/optimize.aspx (Accessed 13 June 2018).Google Scholar
Murray, MG and Thompson, WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8, 4321–4326.Google Scholar
Piepho, HP, Bu, A and Emrich, K (2003) A hitchhiker's guide to mixed models for randomized experiments. Journal of Agronomy & Crop Science 189, 310–322.Google Scholar
Q-bank Bacteria (2017) Q-bank Bacteria Database. Q-bank. Available online from: http://www.q-bank.eu/Bacteria/ (Accessed 13 June 2018).Google Scholar
R Core Team (2016) R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing.Google Scholar
Rivas, R, Martens, M, De Lajudie, P and Willems, A (2009) Multilocus sequence analysis of the genus Bradyrhizobium. Systematic and Applied Microbiology 32, 101–110.Google Scholar
Rodriguez-Navarro, DN, Oliver, IM, Contreras, MA and Ruiz-Sainz, JE (2011) Soybean interactions with soil microbes, agronomical and molecular aspects. Agronomy for Sustainable Development 31, 173–190.Google Scholar
Schmidt, J, Messmer, M and Wilbois, KP (2015) Beneficial microorganisms for soybean (Glycine max (L.) Merr), with a focus on low root-zone temperatures. Plant and Soil 397, 411–445.Google Scholar
Schulz, TJ and Thelen, KD (2008) Soybean seed inoculant and fungicidal seed treatment effects on soybean. Crop Science 48, 1975–1983.Google Scholar
Shenk, JS and Westerhaus, MO (1991 a) Population definition, sample selection, and calibration procedures for near infrared reflectance spectroscopy. Crop Science 31, 469–474.Google Scholar
Shenk, JS and Westerhaus, MO (1991 b) Population structuring of near infrared spectra and modified partial least squares regression. Crop Science 31, 1548–1555.Google Scholar
Stephens, JHG and Rask, HM (2000) Inoculant production and formulation. Field Crops Research 65, 249–258.Google Scholar
Tang, J, Bromfield, ESP, Rodrigue, N, Cloutier, S and Tambong, JT (2012) Microevolution of symbiotic Bradyrhizobium populations associated with soybeans in east North America. Ecology and Evolution 2, 2943–2961.Google Scholar
Thilakarathna, MS and Raizada, MN (2017) A meta-analysis of the effectiveness of diverse rhizobia inoculants on soybean traits under field conditions. Soil Biology and Biochemistry 105, 177–196.Google Scholar
Ulzen, J, Abaidoo, RC, Mensah, NE, Masso, C and AbdelGadir, AH (2016) Bradyrhizobium inoculants enhance grain yields of soybean and cowpea in northern Ghana. Frontiers in Plant Science 7, 1770. DOI: 10.3389/fpls.2016.01770.Google Scholar
USDA-NRCS Soil Science Division (2003) Soil Temperature Regimes Map | NRCS Soils. Washington, DC, USA: USDA. Available online from: https://www.nrcs.usda.gov/wps/portal/nrcs/detail/soils/use/worldsoils/?cid=nrcs142p2_054019 (Accessed 13 June 2018).Google Scholar
Vinuesa, P, Silva, C, Werner, D and Martínez-Romero, E (2005) Population genetics and phylogenetic inference in bacterial molecular systematics: the roles of migration and recombination in Bradyrhizobium species cohesion and delineation. Molecular Phylogenetics and Evolution 34, 29–54.Google Scholar
Vollmann, J, Fritz, CN, Wagentristl, H and Ruckenbauer, P (2000) Environmental and genetic variation of soybean seed protein content under Central European growing conditions. Journal of the Science of Food and Agriculture 80, 1300–1306.Google Scholar
Zhang, H, Daoust, F, Charles, TC, Driscoll, BT, Prithiviraj, B and Smith, DL (2002) Bradyrhizobium japonicum mutants allowing improved nodulation and nitrogen fixation of field-grown soybean in a short season area. Journal of Agricultural Science 138, 293–300.Google Scholar
Zhang, H, Prithiviraj, B, Charles, TC, Driscoll, BT and Smith, DL (2003) Low temperature tolerant Bradyrhizobium japonicum strains allowing improved nodulation and nitrogen fixation of soybean in a short season (cool spring) area. European Journal of Agronomy 19, 205–213.Google Scholar
Zimmer, S, Messmer, M, Haase, T, Piepho, HP, Mindermann, A, Schulz, H, Habekuß, A, Ordon, F, Wilbois, KP and Heß, J (2016) Effects of soybean variety and Bradyrhizobium strains on yield, protein content and biological nitrogen fixation under cool growing conditions in Germany. European Journal of Agronomy 72, 38–46.Google Scholar