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Kinetics of neovascularisation of splenic autotransplants in mice

Published online by Cambridge University Press:  01 October 1999

HUMBERTO J. ALVES ,
GISELE VIANA ,
MÔNICA M. MAGALHÃES ,
ROSA M. E. ARANTES ,
PAULO M. Z. COELHO  and
JOSÉ R. CUNHA-MELO
HUMBERTO J. ALVES
Affiliation:
Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Minais Gerais, Brazil
MÔNICA M. MAGALHÃES
Affiliation:
Department of Surgery, Federal University of Minas Gerais, Belo Horizonte, Minais Gerais, Brazil
ROSA M. E. ARANTES
Affiliation:
Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Minais Gerais, Brazil
PAULO M. Z. COELHO
Affiliation:
Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte, Minais Gerais, Brazil Santa Casa de Misericórdia, Belo Horizonte, Minais Gerais, Brazil
JOSÉ R. CUNHA-MELO
Affiliation:
Department of Surgery, Federal University of Minas Gerais, Belo Horizonte, Minais Gerais, Brazil GEN-CAD Service, Federal University of Minas Gerais, Belo Horizonte, Minais Gerais, Brazil
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Abstract

The aim of this study was to examine the kinetics of neovascularisation of splenic autoimplants into the abdominal cavity after splenectomy in mice. Sixty-eight female Swiss mice were submitted to splenectomy. The spleen from each animal was sliced and the slices were implanted into the abdominal cavity. Groups of animals were killed after 1, 2, 3, 5, 7, 15, 21, 28, 42, 56, 70 and 84 d. Fluorescent polystyrene microspheres were injected via the orbital venous plexus before killing and the splenules were removed 5 min later for light and electron microscopy. Mesenteric blood vessels were injected with coloured latex to study the origin of the nutrient vessels. Three days after the implant the microspheres were observed at the periphery and then migrating to the internal parts of the implant in the subsequent days. The blood supply to the implants originated from branches of the splenic, short gastric, mesenteric and gastroepiploic arteries. It is concluded that revascularisation of splenic autografts proceeds centripetally, starting as early as 3 d after implantation.

Keywords

Vasculaturesplenectomymicrosphere injection
Type
Research Article
Information
The Journal of Anatomy , Volume 195 , Issue 3 , October 1999 , pp. 387 - 392
Copyright
© Anatomical Society of Great Britain and Ireland 1999

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