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A dairy cow model to assess aflatoxin transmission from feed into milk – Evaluating efficacy of the mycotoxin binder Mycosorb®

Published online by Cambridge University Press:  13 December 2013

C.A. Moran ,
H. Kettunen ,
A. Yiannikouris ,
S. Ojanperä ,
E. Pennala ,
I.M. Helander  and
J. Apajalahti
C.A. Moran*
Affiliation:
Alltech France, 25 rue Greffulhe, 92300 Levallois-Perret, France
H. Kettunen
Affiliation:
Alimetrics Ltd, Koskelontie 19 B, FIN-02920 Espoo, Finland
A. Yiannikouris
Affiliation:
Alltech Inc., 3031 Catnip Hill Pike, Nicholasville, KY 40356, USA
S. Ojanperä
Affiliation:
Alimetrics Ltd, Koskelontie 19 B, FIN-02920 Espoo, Finland
E. Pennala
Affiliation:
Alimetrics Ltd, Koskelontie 19 B, FIN-02920 Espoo, Finland
I.M. Helander
Affiliation:
Alimetrics Ltd, Koskelontie 19 B, FIN-02920 Espoo, Finland
J. Apajalahti
Affiliation:
Alimetrics Ltd, Koskelontie 19 B, FIN-02920 Espoo, Finland
*
*Corresponding author:cmoran@alltech.com
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Summary

A dairy cow model was established to measure the transmission of aflatoxin M1 (AFM1) into milk. Four Ayrshire mid-lactation dairy cows (ranging 590 – 650 kg body weight and averaging 4.5 years of age) were used in a crossover experimental design in which each cow acted as its own control and was subjected to four dietary treatments administered as six 12-day feeding periods followed by a 7-day washout period. Periods I – VI comprised a 7 d of adaptation followed by 5 d of sampling. Period VII was a 7-d washout with sampling in the final two days. From each morning's milking, yield was recorded and sampled for AFM1 analysis by HPLC. Treatments included a negative control, contamination with AFB1 (5 µg of AFB1/kg feed), AFB1 + Mycosorb® (MTB) 10 g/cow/d, and AFB1 + MTB 50 g/cow/d. In response to the negative control, AFM1 concentrations in milk remained below the limit of detection (<5 ng/kg), whereas cows fed the AFB1-contaminated feed had milk AFM1 concentrations ranging from 110 to 230 ng/kg. At the end of the washout period, AFM1 was again undetectable. The dairy cow model was also used to test the efficacy of the yeast-cell-wall-based mycotoxin binder Mycosorb® (MTB) in reducing secretion of AFM1 into milk by cows fed the same AFB1-contaminated diet. When supplemented at 50 g/head/day, MTB significantly reduced (P < 0.05) AFM1 secretion into milk with no adverse effects on milk production. The dairy cow model is a sensitive tool for measuring aflatoxin transmission to milk and mycotoxin binder efficacy at 5 µg of AFB1/kg feed. As it is common for on-farm AFB1 concentrations to exceed 5 µg/kg, more research may be warranted to determine the effectiveness of the model at higher AFB1 concentrations.

Keywords

aflatoxin B1aflatoxin M1dairy cow modelMycosorb®milkadsorbentmycotoxin
Type
Original Research
Information
Journal of Applied Animal Nutrition , Volume 2 , 2013 , e6
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2013 

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References

Anfossi, L., Baggiani;, C., Giovannoli, C. & Giraudi, G. (2011) Occurrence of Aflatoxin M1 in Dairy Products, in: Torres-Pacheco, I. (Ed.) Aflatoxins – Detection, Measurement and Control, ISBN:978-953-307-711-6, InTech, Available from: http://www.intechopen.com/books/aflatoxins-detectionmeasurement-and-control/occurrence-of-aflatoxin-m1-in-dairy-productsCrossRefGoogle Scholar
Battacone, G., Nudda, A., Palomba, M., Mazzette, A. & Pulina, G. (2009) The transfer of aflatoxin M1 in milk of ewes fed diet naturally contaminated by aflatoxins and effect of inclusion of dried yeast culture in the diet. J. Dairy Sci. 92: 4997–5004.CrossRefGoogle ScholarPubMed
Britzi, M., Friedman, S., Miron, J., Solomon, R., Cuneah, O., Shimshoni, J.A., Soback, S., Ashkenazi, R., Armer, S. & Shlosberg, A. (2013) Carry-over of aflatoxin B1 to aflatoxin M1 in high yielding Israeli cows in mid- and late- lactation. Toxins, 5: 173–183.CrossRefGoogle ScholarPubMed
Diaz, D.E., Hagler, W.M. JR., Blackwelder, J.T., Eve, J.A., Hopkins, B.A., Anderson, K.L., Jones, F.T. & Whitlow, L.W. (2004) Aflatoxin binders II: reduction of Aflatoxin M1 in milk by sequestering agents of cows consuming Aflatoxin in feed. Mycopathologia, 157: 233–241Google Scholar
Directive 2002/32/EC of the European Parliament and of the Council of 7 May 2002 on undesirable substances in animal feed (2002) Official Journal of the European Union OJ L 140/10Google Scholar
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes Official Journal of the European Union OJ L 276/33.Google Scholar
Dragacci, S., Grosso, F. & Gilbert, J. (2001) Immunoaffinity column cleanup with liquid chromatography for determination of Aflatoxin M1 in liquid milk: collaborative study. Journal of the AOAC International, 84: 437–443CrossRefGoogle ScholarPubMed
EFSA (2010) EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP); Statement on the establishment of guidelines for the assessment of additives from the functional group ‘substances for reduction of the contamination of feed by mycotoxins’. EFSA Journal 8: 1693. [8 pp.]. doi:10.2903/j.efsa.2010.1693.Google Scholar
EFSA (2011) EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP); Technical guidance: Tolerance and efficacy studies in target animals. EFSA Journal 9: 2175. [15 pp.] doi:10.2903/j.efsa.2011.2175.Google Scholar
Fink-Gremmels, J. (2008) Mycotoxins in cattle feeds and carry-over to dairy milk: A review. Food Additives & Contaminants: Part A. 25: 172–180.Google Scholar
Frobish, R.A., Bradley, B.D., Wagner, D.D., Long-Bradley, P.E. & Hairston, H. (1986) Aflatoxin residues in milk of dairy cows after ingestion of naturally contaminated grain. J. Food Protect. 49: 781–785.CrossRefGoogle ScholarPubMed
Jouany, J.P. & Diaz, D. (2005) Effects of mycotoxins in ruminants. Pages 295–321 in The Mycotoxin Blue Book. Diaz, D.E., ed. Nottingham University Press, Nottingham, UK.Google Scholar
Jouany, J.P., Yiannikouris, A. & Bertin, G. (2009) Risk assessment of mycotoxins in ruminants and ruminant products. Options Mediterranéennes, A 85: 205–224.Google Scholar
Kavanagh, G., Pentieva, K., Kennedy, J. & Moran, C.A. (2013) The impact of Regulation (EC) 767/2009 on the practice of feed advertising in Europe. Journal of Applied Animal Nutrition, Vol. 1; e4; page 1 of 10Google Scholar
Kutz, R.E., Sampson, J.D., Pompeu, L.B., Ledoux, D.R., Spain, J.N., Vázquez-Añón, M. & Rottinghaus, G.E. (2009) Efficacy of Solis, NovasilPlus, and MTB-100 to reduce aflatoxin M1 levels in milk of early to mid lactation dairy cows fed aflatoxin B1. J Dairy Sci. 92: 3959–63.Google Scholar
Masoero, F., Gallo, A., Moschini, M., Piva, G. & Diaz, D. (2007) Carryover of aflatoxin from feed to milk in dairy cows with low or high somatic cell counts. Animal 1: 1344–1350.Google Scholar
Moschini, M., Masoero, F., Gallo, A. & Diaz, D. (2007) Mucosal absorption of aflatoxin B1 in lactating dairy cows. Italian Journal Of Animal Science 6 (Suppl. 1): 324–326.Google Scholar
Regulation (EC) no 178/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety (2002) Official Journal of the European Union, OJ L 31/1Google Scholar
Regulation (EC) no 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition (2003) Official Journal of the European Union, L 268/29Google Scholar
Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed. (2009) Official Journal of the European Union L 54/1Google Scholar
Regulation (EC) No 386/2009 of 12 May 2009 amending Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the establishment of a new functional group of feed additives (2009) Official Journal of the European Union OJ L 118/66Google Scholar
Regulation (EC) No 767/2009 of the European Parliament and of the Council of 13 July 2009 on the placing on the market and use of feed, amending European Parliament and Council Regulation (EC) No 1831/2003 and repealing Council Directive 79/373/EEC, Commission Directive 80/511/EEC, Council Directives 82/471/EEC, 83/228/EEC, 93/74/EEC, 93/113/EC and 96/25/EC and Commission Decision 2004/217/EC (2009) Official Journal of the European Union OJ L 229/1.Google Scholar
Regulation (EU) No 165/2010 of 26 February 2010 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards aflatoxins. (2010) Official Journal of the European Union L 50/8.Google Scholar
Regulation (EU) No 68/2013 of 16 January 2013 on the Catalogue of feed materials. (2013) Official Journal of the European Union L29/1.Google Scholar
Rosi, P., Borsari, A., Lasi, G., Lodi, P., Galanti, A., Fava, A., Girotti, S. & Ferri, E. (2007) Aflatoxin M1 in milk: Reliability of the immunoenzymatic assay. International Dairy Journal 17: 429–435.CrossRefGoogle Scholar
SCFCAH (2010) Summary record of the Standing Committee on the Food Chain and Animal Health held on 14 & 15 December 2010 in Brussels. European Commission – DG SANCO. http://ec.europa.eu/food/committees/regulatory/scfcah/animalnutrition/sum_1415122010_en.pdf p. 1–8.Google Scholar
Trunk, W. (2009) Better regulation, simplification and modernisation of the EU-legislation in the area of food safety. European Food and Feed Law Review, 2: 130–134.Google Scholar
Van Egmond, H.P. (1989) Aflatoxin M1: occurrence, toxicity, regulation. In Mycotoxins in dairy products (ed. van Egmond, HP), pp. 11–55. Elsevier Applied Science, London, UK.Google Scholar
Van Egmond, H.P. & Dragacci, S. (2001) Liquid chromatographic method for aflatoxin M1 in milk, in: Trucksess, M.W & Pohland, A.E: (Eds.), Methods in Molecular Biology, Vol. 157 Mycotoxin Protocols II, pp. 59–69 (Totowa, Humana Press).Google Scholar
Völkel, I., Schröer-Merker, E. & Czerny, C-P. (2011) The carry-over of mycotoxins in products of animal origin with special regard to its implications for the European food safety legislation. Food and Nutrition Sciences, 2: 852–867.Google Scholar
Yiannikouris, A.L., Poughon, X., Cameleyre, C.-G., Dussap, J., François, G. Bertin, & Jouany, J.-P.. (2003) A novel technique to evaluate interactions between Saccharomyces cerevisiae cell wall and mycotoxins: application to zearalenone. Biotechnology Letters 25: 783–789.CrossRefGoogle ScholarPubMed