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Alternative strategies for activation of the natural lactoperoxidase system in cows' milk: trials in Tanzania

Published online by Cambridge University Press:  09 July 2007

Leonard WT Fweja ,
Michael J Lewis  and
Alistair S Grandison
Leonard WT Fweja
Affiliation:
Department of Food Biosciences, The University of Reading, P O Box 226, Whiteknights, Reading, RG6 6AP, UK
Michael J Lewis
Affiliation:
Department of Food Biosciences, The University of Reading, P O Box 226, Whiteknights, Reading, RG6 6AP, UK
Alistair S Grandison*
Affiliation:
Department of Food Biosciences, The University of Reading, P O Box 226, Whiteknights, Reading, RG6 6AP, UK
*
*For correspondence; e-mail: a.s.grandison@reading.ac.uk
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Abstract

Thiocyanate content and lactoperoxidase activity of individual cow's milk of different breeds were determined, and the effects of different lactoperoxidase system (LP-s) activation strategies were compared. Lactoperoxidase activity varied significantly between Friesian and both Ayrshire and Tanzania Short Horn Zebu (TSHZ), but differences between Ayrshire and TSHZ were not significant. There was no significant variation in SCN− content between breeds. The LP-s was activated using three strategies based on SCN−: namely; equal concentrations of SCN− and H2O2 (7:7, 10:10, 15:15 mg/l), excess SCN− concentrations (15:10, 20:10, 25:10 mg SCN−:H2O2/l), and excess H2O2 concentrations (10:15, 10:20, 10:25 mg SCN−:H2O2/l), plus a fourth strategy based on I− (15:15 mg I−:H2O2/l). The keeping quality (KQ) was assessed using pH, titratable acidity, clot on boiling and alcohol stability tests. All activation strategies enhanced the shelf life of milk (typically increasing KQ from around 10 to around 20 h), but it was clear that the effectiveness of the LP-s depends on the type and concentrations of the activators of the system. The LP-s activated using I− as an electron donor was more effective than the LP-s activated using SCN− as an electron donor, increasing the KQ by a further 6–8 h compared with SCN−.

Keywords

Lactoperoxidasethiocyanateiodidehydrogen peroxidecow milkkeeping quality
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 4 , November 2007 , pp. 381 - 386
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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References

Barraquio, VL, Resubal, LE, Bantoc, IBM & Almazan, EN 1994 Preservation of raw milk with lactoperoxidase/hydrogen peroxide/thiocyanate system, hydrogen system and by refrigeration. Asia Life Sciences 3 1–10Google Scholar
Barret, NE, Grandison, AS & Lewis, MJ 1999 Contribution of the lactoperoxidase system to the keeping quality of pasteurised milk. Journal of Dairy Research 66 73–80CrossRefGoogle Scholar
Björck, L 1978 Antibacterial effect of lactoperoxidase system on psychrotrophic bacteria in milk. Journal of Dairy Research 45 109–118CrossRefGoogle ScholarPubMed
Björck, L, Claesson, O & Schulthess, W 1979 The lactoperoxidase/thiocyanate/hydrogen peroxide system as a temporary preservative for raw milk in developing countries. Milchwissenschaft 34 726–729Google Scholar
Björck, L 1981 Activation of lactoperoxidase system as a mean of preventing bacterial deterioration of raw milk – Invited paper IDF symposium on Bacteriological Quality of raw milkGoogle Scholar
Björck, L 1985 The Lactoperoxidase System. In Antimicrobial Systems in Milk. Proceedings of the International Dairy Federation Symposium on Natural Antimicrobial Systems. Bath University Press UKGoogle Scholar
Bosch, EH, Van Doorne, H & De Vries, S 2000 The lactoperoxidase system: the influence of iodide and the chemical and antimicrobial stability over the period of about 18 months. Journal of applied Microbiology 89 215–224CrossRefGoogle ScholarPubMed
Chakraborty, BK, Chaudry, SS, Alex, KA, Jacob, G & Soni, GJ 1986 Application of lactoperoxidase system for preserving buffalo milk produced in Indian villages. Milchwissenschaft 41 16–19Google Scholar
De Wit, JN & van Hooydonk, ACM 1996 Structure, functions, and application of lactoperoxidase in natural antimicrobial systems. Netherlands Milk and Dairy Journal 50 227–244Google Scholar
Ewais, SM, Sh Hefnawy, A & Abd El-Salam, MH 1985 Utilisation of lactoperoxidase system in preservation of raw milk under local conditions. Egyptian Journal of Dairy Science 13 1–7Google Scholar
FAO 1996 Food fortification: technology and quality control. FAO food and nutrition paper number 60 FAO RomeGoogle Scholar
FAO/WHO 1991 Guidelines for the preservation of raw milk by use of the lactoperoxidase system CAC/GL 13-1991Google Scholar
Fonteh, FA, Grandison, AS & Lewis, MJ 2002 Variation of lactoperoxidase activity and thiocyanate content in cows' and goats' milk throughout lactation. Journal of Dairy Research 69 401–409CrossRefGoogle ScholarPubMed
Korhonen, H 1973 Untersuchungen zur bakterizidie der milch und immunisierung der bovinen milchdrüse Meijeritieteellinen Aikakauskirja (Finnish Journal of Dairy Science) 32 (3) 1–15Google Scholar
Korhonen, H 2004 The lactoperoxidase system in mastitic milk. FAO LP Expert Meeting 29 February–1 March 2004, Cape Town South AfricaGoogle Scholar
Reiter, B & Harnulv, BG 1984 Lactoperoxidase antibacterial system: Natural occurrence, biological functions and practical applications. Journal of Food Protection 47 724–732CrossRefGoogle ScholarPubMed
Ridley, SC & Shalo, PL 1990 Farm Application of Lactoperoxidase Treatment and Evaporative Cooling for the intermediate Preservation of Unprocessed Milk in Kenya. Journal of Food Protection 53 592–597CrossRefGoogle ScholarPubMed
Rossi, EA & de Oliveira, JS 1993/94 Effect of thiocyanate concentration on the effectiveness of the lactoperoxidase system activated in raw milk. Alimentos-e-Nutriecao 5 49–56Google Scholar
Rossi, EA, Oliveira, JS & Faria, JB 1995 Effect of hydrogen peroxide levels on the effectiveness of the lactoperoxidase system in raw milk. Ciencie-e-Technologia-de-Alimentos 14 178–188Google Scholar
Seif, E, Buys, EM & Donkin, EF 2005 Significance of lactoperoxidase system in the dairy industry and its potential application: a review. Trends in Food Science and Technology 16 137–154CrossRefGoogle Scholar
Shin, K, Hayasawa, H & Lönnerdal, B 2001 Inhbition of Escherichia Coli respiratory enzymes by the lactoperoxidase – hydrogen peroxide-thiocyanate antimicrobial system. Journal of applied Microbiology 90 489–493CrossRefGoogle Scholar
Siva, CV, Upadhyay, KG & Sannabhadti, SS 1991 Lactoperoxidase/thiocyanate/hydrogen peroxide system, its use and implication in manufacture of dairy products. Indian dairyman 43 240–246Google Scholar
Thaka, RP & Dave, JM 1986 Application of the activated lactoperoxidase-thiocyanate-hdrogen peroxide system in enhancing the keeping quality of buffalo milk at higher temperatures. Milchwissenschaft 41 20–22Google Scholar
Wolfson, LM and Sumner, SS 1993 Antibacterial activity of the Lactoperoxidase system: a Review. Journal of Food Protection 56 887–892CrossRefGoogle ScholarPubMed