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Association between the GHR, GHRHR and IGF1 gene polymorphisms and milk coagulation properties in Sarda sheep

Published online by Cambridge University Press:  10 July 2019

Maria L. Dettori ,
Michele Pazzola ,
Emanuela Pira ,
Giorgia Stocco  and
Giuseppe M. Vacca
Maria L. Dettori*
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Michele Pazzola
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Emanuela Pira
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Giorgia Stocco
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
Giuseppe M. Vacca
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy
*
Author for correspondence: Maria L. Dettori, Email: mldettori@uniss.it
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Abstract

We investigated whether variation of the sheep Growth Hormone Receptor (GHR), Growth Hormone Releasing Hormone Receptor (GHRHR) and Insulin-Like Growth Factor 1 (IGF1) genes were associated with milk coagulation properties (MCP) in sheep. The GHR, GHRHR and IGF1 genes are part of the GH system, which is known to modulate metabolism, growth and reproduction as well as mammogenesis and galactopoiesis in dairy species. A total of 380 dairy Sarda sheep were genotyped for 36 SNPs mapping to these three genes. Traditional MCP were measured as rennet coagulation time (RCT), curd-firming time (k20) and curd firmness at 30 m (a30). Modeling of curd firming over time (CFt) was based on a 60 m lactodynamographic test, generating a total of 240 records of curd firmness (mm) for each milk sample. The model parameters obtained included: the rennet coagulation time as a result of modeling all data available (RCTeq, min); the asymptotic potential value of curd firmness (CFP, mm) at an infinite time; the CF instant rate constant (kCF, %/min); the syneresis instant rate constant (kSR, %/min); the maximum value of CF (CFmax, mm) and the time at achievement of CFmax (tmax, min). Statistical analysis revealed that variation of the GHR gene was significantly associated with RCT, kSR and CFP (P < 0.05). No other significant associations were detected. These findings may be useful for the dairy industry, as well as for selection programs.

Keywords

GHRGHRHRIGF1milk coagulation propertiessheep milk
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 3 , August 2019 , pp. 331 - 336
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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