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Chromosomal location of the genetic determinants for bacteriocins produced by Lactobacillus gasseri K7

Published online by Cambridge University Press:  09 May 2003

Andreja Čanžek Majhenič ,
Bojana Bogovič Matijašić  and
Irena Rogelj
Andreja Čanžek Majhenič
Affiliation:
University of Ljubljana, Biotechnical Faculty, Chair of Dairy Science, Groblje 3, SI-1230 Domžale, Slovenia
Bojana Bogovič Matijašić
Affiliation:
University of Ljubljana, Biotechnical Faculty, Chair of Dairy Science, Groblje 3, SI-1230 Domžale, Slovenia
Irena Rogelj
Affiliation:
University of Ljubljana, Biotechnical Faculty, Chair of Dairy Science, Groblje 3, SI-1230 Domžale, Slovenia
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Abstract

The production of similar or even identical bacteriocins by different lactic acid bacteria is not a rare event. To take advantage of this finding, genetic determinants of the Lactobacillus K7 bacteriocins were tested for putative homologies with previously described bacteriocins of the Lactobacillus acidophilus group through polymerase chain reaction (PCR). Among specific primer pairs of seven known bacteriocins, derived from their respective sequences, only acidocin LF221 A and B primers amplified fragments in chromosomal DNA of K7 strain that revealed strong similarity over small regions of LF221 bacteriocins. Treatment of Lactobacillus K7 with ethidium bromide and mitomycin C was ineffective in generating non-bacteriocinogenic derivatives and had no impact on plasmid loss either. Classification studies elucidated Lactobacillus K7 as a member of the Lactobacillus gasseri species.

Keywords

BacteriocinLactobacillus gasseriLactobacillus acidophilus groupPCR
Type
Research Article
Information
Journal of Dairy Research , Volume 70 , Issue 2 , May 2003 , pp. 199 - 203
Copyright
Proprietors of Journal of Dairy Research 2003

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