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Exogenous phospholipase A2 affects inflammatory gene expression in primary bovine mammary epithelial cells

Published online by Cambridge University Press:  30 April 2019

Jacqueline P. Kurz ,
Mark P. Richards ,
Matthew Garcia  and
Zhongde Wang
Jacqueline P. Kurz
Affiliation:
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84341, USA Utah Veterinary Diagnostic Laboratory, Utah State University, Logan 84341, USA School of Veterinary Medicine, Utah State University, Logan 84341, USA
Mark P. Richards
Affiliation:
Department of Animal Sciences, Meat Science and Muscle Biology Laboratory, University of Wisconsin-Madison, Madison, WI 53705, USA
Matthew Garcia
Affiliation:
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84341, USA
Zhongde Wang*
Affiliation:
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84341, USA
*
Author for correspondence: Zhongde Wang, Email: zonda.wang@usu.edu
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Abstract

This Research Communication addresses the hypothesis that exogenously administered phospholipase A2 (PLA2) affects the inflammatory responses of bovine mammary epithelial cells (bMEC) in vitro with the aim of providing preliminary justification of investigation into the uses of exogenously administered PLA2 to manage or treat bovine mastitis. Primary bMEC lines from 11 lactating Holstein dairy cows were established and the expression of 14 pro-inflammatory genes compared under unchallenged and lipopolysaccharide (LPS)-challenged conditions, with and without concurrent treatment with bovine pancreatic PLA2G1B, a secreted form of PLA2. No differences in the expression of these genes were noted between PLA2-treated and untreated bMEC under unchallenged conditions. Following LPS challenge, untreated bMEC exhibited significant downregulation of CXCL8, IL1B, CCL20, and CXCL1. In contrast, PLA2-treated bMEC exhibited significant downregulation of IL1B and CCL20 only. These findings indicate that exogenous PLA2 affects the expression of some pro-inflammatory factors in immune-stimulated bMEC, but does not influence the constitutive expression of these factors. Further investigation of the influence of exogenous PLA2 in the bovine mammary gland is justified.

Keywords

Bovine mammary epithelial cellHolsteinmastitisphospholipase A2
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 177 - 180
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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