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Improved detection of Mycobacterium paratuberculosis in milk

Published online by Cambridge University Press:  14 July 2005

Eva D'Haese ,
Iris Dumon ,
Hadewig Werbrouck ,
Valerie De Jonghe  and
Lieve Herman
Eva D'Haese
Affiliation:
Scientific Institute of Public Health, Juliette Wytsmanstraat 14, B-1050 Brussels, Belgium
Iris Dumon
Affiliation:
Department for Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium
Hadewig Werbrouck
Affiliation:
Department for Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium
Valerie De Jonghe
Affiliation:
Department for Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium
Lieve Herman
Affiliation:
Department for Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium
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Abstract

At present there is no rapid microbiological method for the detection of viable Mycobacterium paratuberculosis in milk. By combining an extensive milk sample pretreatment with solid phase cytometry as the detection technique we were able to demonstrate viable mycobacterial cells in 50 ml of artificially contaminated pasteurized milk in less than one working day.

Keywords

Mycobacterium paratuberculosissolid phase cytometrymilk
Type
Research Article
Information
Journal of Dairy Research , Volume 72 , Issue S1 , August 2005 , pp. 125 - 128
Copyright
Proprietors of Journal of Dairy Research 2005

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