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Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live lactococci on the mammary immune response

Published online by Cambridge University Press:  04 August 2008

Fiona Crispie ,
Mercedes Alonso-Gómez ,
Collette O'Loughlin ,
Katja Klostermann ,
James Flynn ,
Seán Arkins ,
William Meaney ,
R Paul Ross  and
Colin Hill
Fiona Crispie
Affiliation:
Biotechnology Department, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland Dairy Production Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
Mercedes Alonso-Gómez
Affiliation:
Department of Life Sciences, University of Limerick, Limerick, Ireland
Collette O'Loughlin
Affiliation:
Department of Life Sciences, University of Limerick, Limerick, Ireland
Katja Klostermann
Affiliation:
Biotechnology Department, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland Dairy Production Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
James Flynn
Affiliation:
Dairy Production Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
Seán Arkins
Affiliation:
Department of Life Sciences, University of Limerick, Limerick, Ireland
William Meaney
Affiliation:
Dairy Production Research Centre, Moorepark, Fermoy, Co. Cork, Ireland
R Paul Ross*
Affiliation:
Biotechnology Department, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland
Colin Hill
Affiliation:
Department of Microbiology, University College Cork, Cork, Ireland
*
*For correspondence; e-mail: paul.ross@teagasc.ie
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Abstract

In the accompanying article, we demonstrated that a live culture of Lactococcus lactis compares favourably with antibiotics for treatment of bovine mastitis in two initial field trials. In an effort to explain the mechanism involved, this study investigated the effect of culture administration on the local immune response. In this respect we initially observed that infusion of the live culture Lactococcus lactis stimulated substantial recruitment of polymorphonucleocytes (PMN) and lymphocytes to the udder. For instance, in one assay, quarters infused with the probiotic experienced a dramatic increase (~20 000-fold) in neutrophils over the first 48-h period from an average value of 83·6 cells/ml pre-treatment to 1·78×106 cells/ml 48 h post-infusion. Levels of the acute phase proteins haptaglobin and milk amyloid A were also elevated significantly in comparison with controls following infusion of the culture. The results of flow cytometric assays also demonstrated that while infusion of a live lactococcal culture led to an enhanced recruitment of PMN to the udder (from 1·85×104 cells/ml pre-infusion to 1·45×106 cells/ml 24 h post-infusion) cell-free supernatant from the same culture was not able to do so, indicating that live Lc. lactis can specifically trigger the mammary immune response to elicit PMN accumulation. These results suggest that the mechanism responsible for this probiotic treatment of mastitis is associated with stimulation of the host intramammary immune system.

Keywords

Lactococcusmastitistreatmentimmune response
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 3 , August 2008 , pp. 374 - 384
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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