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Iron-supplemented caseins: preparation, physicochemical characterization and stability

Published online by Cambridge University Press:  01 June 2009

Frédéric Gaucheron ,
Marie-Hélène Famelart  and
Yvon Le Graët
Frédéric Gaucheron
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherches de Technologie Laitière, 65 rue de Saint-Brieuc, F-35042 Rennes Cedex, France
Marie-Hélène Famelart
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherches de Technologie Laitière, 65 rue de Saint-Brieuc, F-35042 Rennes Cedex, France
Yvon Le Graët
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherches de Technologie Laitière, 65 rue de Saint-Brieuc, F-35042 Rennes Cedex, France
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Summary

Preparation, physicochemical characterization and stability of iron-supplemented caseins were studied. When ferrous iron (final concentrations of 0·25, 0·5, 1·0 and 1·5 mM) was added to sodium caseinate (final casein concentration 25 g/l), iron binding to caseins was complete. In parallel, an increase in absorbance at 280 nm, a decrease in fluorescence intensity, modifications of reversed-phase HPLC profile, a decrease in pH and an increase in free sodium concentration were observed. Moreover, no release of iron from iron-casein complexes was found between pH 6·5 and 3·7 or after heat treatments or in the presence of inorganic phosphate. Only EDTA disodium salt and trisodium citrate had significant effects on the release of iron bound to caseins.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 2 , May 1996 , pp. 233 - 243
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

REFERENCES

Albright, K. J., Gordon, D. T. & Cotterill, O. J. 1984 Release of iron from phosvitin by heat and food additives. Journal of Food Science 49 78–81CrossRefGoogle Scholar
Brignon, G., Ribadeau-Dumas, B., Mercier, J. -C., Pelissier, J. -P. & Das, B. C. 1977 Complete amino acid sequence of bovine αs2 casein. FEBS Letters 76 274–279CrossRefGoogle ScholarPubMed
Demott, B. J. & Dincer, B. 1976 Binding added iron to various milk proteins. Journal of Dairy Science 59 1557–1559CrossRefGoogle ScholarPubMed
Emery, T. 1992 Iron oxidation by casein. Biochemical and Biophysical Research Communications 182 1047–1052CrossRefGoogle ScholarPubMed
Furia, T. E. 1972 Sequestrants in food. In CRC Handbook of Food Additives. 2nd edn, vol. I, p. 275. Cleveland, OH: Chemical Rubber Co.Google Scholar
Hegenauer, J., Saltman, P., Ludwig, D., Ripley, L. & Ley, A. 1979 Iron-supplemented cow milk. Identification and spectral properties of iron bound to casein micelles. Journal of Agricultural and Food Chemistry 27 1294–1301CrossRefGoogle ScholarPubMed
Jackson, S. L. & Lee, K. 1992 The effect of dairy products on iron availability. Critical Reviews in Food Science and Nutrition 31 259–270CrossRefGoogle ScholarPubMed
Jaubert, A. & Martin, P. 1992 Reverse-phase HPLC analysis of goat caseins. Identification of αsl and αs2 genetic variants. Lait 72 235–247CrossRefGoogle Scholar
Mac Phail, A. P., Patel, R. C., Bothwell, T. H. & Lamparelli, R. D. 1994 EDTA and the absorption of iron from food. American Journal of Clinical Nutrition 59 644–648CrossRefGoogle ScholarPubMed
Manson, W. & Cannon, J. 1978 The reaction of αs1-and β-casein with ferrous ions in the presence of oxygen. Journal of Dairy Research 45 59–67CrossRefGoogle Scholar
Ribadeau-Dumas, B., Brignon, B., Grosclaude, F. & Mercier, J. C. 1968 [Primary structure of bovine β-casein. Complete sequence.] European Journal of Biochemistry 25 505–514CrossRefGoogle Scholar
Schuette, H. A. 1939 Alimentary and medicinal uses ot casein. In Casein and its Industrial Applications, 2nd edn, pp. 366–390 (Eds Sutermeister, E. and Browne, F. L.). New York: ReinholdGoogle Scholar
Snow Brand Milk Products 1990 a Compositions for therapy of iron deficiency – containing iron casein as active substance. Japanese Patent JP 02083333 AGoogle Scholar
Snow Brand Milk Products 1990 b Iron casein preparation, useful in treating anaemia – by reacting ferrous salt aqueous solution with soluble casein aqueous solution. Japanese Patent JP 02083340 AGoogle Scholar
Swaisgood, H. E. 1982 Chemistry of milk proteins. In Developments in Dairy Chemistry—1. Proteins, pp. 1–59 (Ed. Fox, P. F.). London: Applied ScienceGoogle Scholar
Visser, S., Slangen, C. J. & Rollema, H. S. 1991 Phenotyping of bovine milk proteins by reversed-phase high-performance liquid chromatography. Journal of Chromatography 548 361–370CrossRefGoogle ScholarPubMed
Webb, J., Multani, J. S., Saltman, P., Beach, N. A. & Gray, H. B. 1973 Spectroscopic and magnetic studies of iron (III) phosvitins. Biochemistry 12 1797–1802CrossRefGoogle Scholar