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AGPAT3 Gene polymorphisms are associated with milk production traits in Chinese Holstein cows

Published online by Cambridge University Press:  15 July 2021

Xiaomei Sun ,
Yan Liang ,
Qisong Gao ,
Jiahe Guo ,
Cheng Tang ,
Kerong Shi ,
Zhangping Yang  and
Yongjiang Mao
Xiaomei Sun*
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Yan Liang
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Qisong Gao
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Jiahe Guo
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Cheng Tang
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China
Kerong Shi
Affiliation:
College of Animal Science and Technology, Shandong Agricultural University, Taian271018, China
Zhangping Yang
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou225009, China
Yongjiang Mao*
Affiliation:
Key Laboratory of Animal Genetics & Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou225009, China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou225009, China
*
Authors for correspondence: Xiaomei Sun, Email: sunxiaomei@live.com Yongjiang Mao, E-mail: cattle@yzu.edu.cn
Authors for correspondence: Xiaomei Sun, Email: sunxiaomei@live.com Yongjiang Mao, E-mail: cattle@yzu.edu.cn
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Abstract

The current study reports the identification of previously undiscovered single-nucleotide polymorphisms (SNPs) in the bovine AGPAT3 gene and further investigates their associations with milk production traits. Our results demonstrate that the major allele C of the SNP g.12264 C > T is positively correlated with test-day milk yield, protein percentage and 305-day milk yield. Importantly, in silico analysis showed that the C/T transition at this locus gives rise to two new transcription factor binding sites (TFBS), E2F1 and Nkx3-2. Polymorphism g.18658 G > A was the only SNP associated with milk urea nitrogen (MUN) with the G allele related to an increase in milk urea nitrogen as well as fat percentage. The GG genotype of SNP g.28731 A > G was associated with the highest fat and protein percentage and lowest 305-day milk yield and somatic cell score (SCS). The association between AGPAT3 locus and milk production traits could be utilized in marker-assisted selection for the genetic improvement of milk production traits and, probably in conjunction with other traits, for selection to improve fitness of dairy cattle.

Keywords

1-acylglycerol-3-phosphate O-acyltransferase 3AssociationDairy cattleMilk production traitsSingle nucleotide polymorphisms
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 247 - 252
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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