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Anti-atopic dermatitis effects and the mechanism of lactic acid bacteria isolated from Mongolian fermented milk

Published online by Cambridge University Press:  05 January 2009

Atsushi Hayashi ,
Makoto Kimura ,
Yusaku Nakamura  and
Hisako Yasui
Atsushi Hayashi
Affiliation:
Science of Functional Foods Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan
Makoto Kimura
Affiliation:
Science of Functional Foods Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan
Yusaku Nakamura
Affiliation:
Science of Functional Foods Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan
Hisako Yasui*
Affiliation:
Science of Functional Foods Graduate School of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan
*
*For correspondence; e-mail: hisakoy@shinshu-u.ac.jp
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Abstract

We investigated the anti-allergic effects of one strain (T120) of a lactic acid bacteria (LAB) isolated from Mongolian fermented milk using atopic dermatitis (AD) model mice (NC/Nga mice). Strain T120 has already been identified as Enterococcus faecium and shown to induce strong production of IL-12 (Kimura et al. 2006). In in vitro studies, strain T120 suppressed total IgE production and induced IL-12 and IFN-γ production by splenocytes of NC/Nga mice. The additional examination of various neutralization antibodies was performed to elucidate in detail the mechanism of depressed IgE production by strain T120. As a result, it became clear that IL-12 induced by strain T120 increased production of IFN-γ and total IgE production was mainly controlled by the IFN-γ. In order to define the cells which produce IL-12 powerfully by this strain, antigen-presenting cells (APCs) such as macrophages and dendritic cells (DCs) were removed from the splenocytes, and the reactivity of these cells to the strain was examined. Induction of IL-12 and IFN-γ by strain T120 became significantly very low by removal of APCs from splenocytes. Therefore, it was clear that strain T120 acted on APCs and induced production of IL-12. Further, this strain enhanced the production of IL-10 by splenocytes. In in vivo studies, intraperitoneal injection of strain T120 inhibited serum IgE elevation and atopic dermatitis symptoms in NC/Nga mice. These results suggest that an anti-allergic effect of strain T120 depends on the increased production of IL-12 by APCs activated by the strain and following the increased production of IFN-γ. Further, activation of regulatory T cells by strain T120 may inhibit atopic desease.

Keywords

NC/Nga mouseatopic dermatitislactic acid bacteriaanti-allergy
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 158 - 164
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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