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Anti-inflammatory effect of yoghurt in an experimental inflammatory bowel disease in mouse

Published online by Cambridge University Press:  07 November 2008

Nadia Gobbato ,
Mirta Rachid  and
Gabriela Perdigón
Nadia Gobbato
Affiliation:
Instituto de Microbiología “Dr. Luis C. Verna”, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 471-2° piso. Tucumán (4000). Argentina
Mirta Rachid
Affiliation:
Instituto de Microbiología “Dr. Luis C. Verna”, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 471-2° piso. Tucumán (4000). Argentina
Gabriela Perdigón*
Affiliation:
Instituto de Microbiología “Dr. Luis C. Verna”, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 471-2° piso. Tucumán (4000). Argentina Centro de Referencia para Lactobacilos – CERELA – CONICETChacabuco 149. Tucumán (4000). Argentina
*
*For correspondence; e-mail: perdigon@cerela.org.ar
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Abstract

Inflammatory bowel disease (IBD; Crohn′s disease and ulcerative colitis) is the clinical outcome of three interactive pathogenic factors: genetic susceptibility, environmental triggers and immune dis-regulation. At present, only the immune response is targeted by most therapeutic or preventive strategies. The beneficial effect of yoghurt on health as well as its immunomodulator effect on the gut immune system is well documented. The aim of this work was to study the possible beneficial effects of yoghurt consumption on an experimental model of IBD in mice. Balb/c mice were fed with yoghurt for 10 consecutive days. At the end of the feeding period the mice received three inoculations of 2, 4, 6-trinitrobenzene sulphonic acid (TNBS) solutions once a week for 3 consecutive weeks. After TNBS instillation the mice received yoghurt again for 10 consecutive days. IBD control received only TNBS. After treatments we analysed the number of IgA–secreting cells, CD4+, CD8+ T cells population and the number of apoptotic cells in the large intestine. The number of erythrocytes and leucocytes in peripheral blood mononuclear cells (PBMCs) was also determined. We demonstrated the antinflammatory effect of yoghurt in an experimental model of IBD induced by TNBS. The effect was mediated by an increase in the number of the IgA+cells, a decrease in CD8+ population and by the induction of apoptosis of the infiltrative cells in the large intestine.

Keywords

inflammatory bowel disease- yoghurt-TNBS- apoptosis
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 4 , November 2008 , pp. 497 - 504
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Blumberg, RS & Strober, W 2001 Prospects for research in inflammatory bowel disease. The Journal of the American Association 285 643–647CrossRefGoogle ScholarPubMed
Boirivant, M, Marini, M, Di Felice, G, Pronio, AM, Montesani, C, Tersigni, R & Strober, W 1999 Lamina propria T cells in Crohn′s disease and other gastrointestinal inflammation show defective CD2 pathway-induced apoptosis. Gastroenterology 116 557–565CrossRefGoogle ScholarPubMed
Chin, KW & Barrett, KE 1994 Mast cells are not essential to inflammation in murine model of colitis. Digestive Diseases and Sciences 39 513–525CrossRefGoogle Scholar
Fiocchi, C 1998 Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology 115 182–205CrossRefGoogle ScholarPubMed
Fuss, IJ, Marth, T, Neurath, MF, Pearlstein, GR, Jain, A & Strober, W 1999 Anti-interleukin 12 treatment regulates apoptosis of Th1 cells in experimental colitis in mice. Gastroenterology 117 1078–1088CrossRefGoogle ScholarPubMed
Gobbato, N, Maldonado Galdeano, C & Perdigon, G 2008 Study of some of the mechanisms involved in the prevention against Salmonella enteritidis serovar Typhimurium infection by lactic acid bacteria. Food and Agricultural Immunology 19 17–23CrossRefGoogle Scholar
Guarner, F & Schaafsma, G 1998 Probiotics. International Journal of Food Microbiology 39 237–238CrossRefGoogle ScholarPubMed
Isaacson, P 1982 Immunoperoxidase study of the secretory immunoglobulin system in colonic neoplasia. Journal of Clinical Pathology 35 14–25CrossRefGoogle ScholarPubMed
Isolauri, E 2001 Probiotics in human disease. The American Journal of Clinical Nutrition 73 1142S–1146SCrossRefGoogle ScholarPubMed
Isolauri, E, Sütas, Y, Kankaanpää, P, Arvilommi, H & Salminen, S 2001 Probiotics: effects on immunity. The American journal of clinical nutrition 73 444S–450SCrossRefGoogle ScholarPubMed
Macpherson, AJ, Gatto, D, Sainsbury, E, Harriman, GR, Hengartner, H & Zinkernagel, RM 2000 A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria. Science 288 2222–2226CrossRefGoogle ScholarPubMed
Madsen, KL, Doyle, JS, Jewell, LD, Tavernini, MM & Fedorak, RN 1999 Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. Gastroenterology 116 1107–1114CrossRefGoogle ScholarPubMed
Maldonado Galdeano, CM, de Moreno, de LeBlanc A, Vinderola, G, Bonet, ME & Perdigón, G 2007 Prosposed Model: Mechanisms of immunomodulation induced by probiotic bacteria. Clinical and Vaccine Immunology 14 485–492CrossRefGoogle Scholar
Malin, M, Suomalainen, H, Saxelin, M & Isolauri, E 1996 Promotion of IgA immune response in patients with Crohn′s disease by oral bacteriotherapy with Lactobacillus GG. Annals of Nutrition & Metabolism 40 137–145CrossRefGoogle ScholarPubMed
Müller, S, Lory, J, Corazza, N, Griffiths, GM, Z'graggen, K, Mazzucchelli, L, Kappeler, A & Mueller, C 1998 Activated CD4+ and CD8+ cytotoxic cells are present in increased numbers in the intestinal mucosa from patients with active inflammatory bowel disease. The American Journal of Pathology 152 261–268.Google ScholarPubMed
Perdigón, G, Alvarez, S, Rachid, M, Agüero, G & Gobbato, N 1995 Immune system stimulation by probiotic. Journal of Dairy Science 78 1597–1606CrossRefGoogle Scholar
Perdigón, G, de Moreno, de LeBlanc A, Valdez, J & Rachid, M 2002 Role of yoghurt in the prevention of colon cancer. European Journal of Clinical Nutrition 56 S65–68CrossRefGoogle ScholarPubMed
Perdigón, G, Rachid, M, De Budeguer, MV & Valdez, JC 1994 Effect of yogurt feeding on the small and large intestine associated lymphoid cells in mice. Journal of Dairy Research 61 553–562CrossRefGoogle ScholarPubMed
Perdigón, G, Valdez, JC & Rachid, M 1998 Antitumor activity of yogurt: study of the possible immune mechanisms. Journal of Dairy Research 65 129–138CrossRefGoogle Scholar
Pirzer, U, Schänhaar, A, Fleischer, B, Hermann, E & Meyer zum Büschenfelde, KH 1991 Reactivity of infiltrating T lymphocytes with microbial antigens in Crohn's disease. Lancet 338 1238–1239CrossRefGoogle ScholarPubMed
Rachid, MM, Gobbato, NM, Valdéz, JC, Vitalone, HH & Perdigón, G 2002 Effect of yogurt on the inhibition of an intestinal carcinoma by increasing cellular apoptosis. International Journal of Immunopathology and Pharmacology 15 209–216CrossRefGoogle ScholarPubMed
Saikumar, P, Dong, Z, Mikhailov, V, Denton, M, Weinberg, JM & Venkatachalam, MA 1999 Apoptosis: definition, mechanisms, and relevance to disease. The American Journal of Medicine 107 489–506.Google Scholar
Sainte-Marie, G 1962 A paraffin embedding technique for studies employing immunofluorescence. The Journal of Histochemistry and Cytochemistry 10 250–256CrossRefGoogle Scholar
Salminen, S, Bouley, C, Boutron-Ruault, MC, Cummings, JH, Franck, A, Gibson, GR, Isolauri, E, Moreau, MC, Roberfroid, M & Rowland, I 1998 Functional food science and gastrointestinal physiology and function. British Journal of Nutrition 80 S147–171CrossRefGoogle ScholarPubMed
Shanahan, F 2001 Inflammatory bowel disease: immunodiagnostics, immunotherapeutics, and ecotherapeutics. Gastroenterology 120 622–635CrossRefGoogle ScholarPubMed
Singh, B, Read, S, Asseman, C, Malmström, V, Mottet, C, Stephens, LA, Stepankova, R, Tlaskalova, H & Powrie, F 2001 Control of intestinal inflammation by regulatory T cells. Immunological Reviews 182 190–200CrossRefGoogle ScholarPubMed
Steidler, L 2001 Microbiological and immunological strategies for treatment of inflammatory bowel disease. Microbes and infection 3 1157–1166CrossRefGoogle ScholarPubMed