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Comparison of the activity of human and bovine milk on two cell lines

Published online by Cambridge University Press:  18 May 2009

Coloma Pocoví ,
Celia Conesa ,
Chockry Barbana ,
María D Pérez ,
Miguel Calvo  and
Lourdes Sánchez
Coloma Pocoví
Affiliation:
Tecnología de los Alimentos, Facultad de Veterinaria, Miguel Servet, 177, 50013Zaragoza, Spain
Celia Conesa
Affiliation:
Tecnología de los Alimentos, Facultad de Veterinaria, Miguel Servet, 177, 50013Zaragoza, Spain
Chockry Barbana
Affiliation:
Tecnología de los Alimentos, Facultad de Veterinaria, Miguel Servet, 177, 50013Zaragoza, Spain
María D Pérez
Affiliation:
Tecnología de los Alimentos, Facultad de Veterinaria, Miguel Servet, 177, 50013Zaragoza, Spain
Miguel Calvo
Affiliation:
Tecnología de los Alimentos, Facultad de Veterinaria, Miguel Servet, 177, 50013Zaragoza, Spain
Lourdes Sánchez*
Affiliation:
Tecnología de los Alimentos, Facultad de Veterinaria, Miguel Servet, 177, 50013Zaragoza, Spain
*
*For correspondence; e-mail: lousanchez@unizar.es
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Abstract

The activity of human milk on cell growth has been evaluated on two cell lines, MDCK and Caco-2. The proportion of human milk samples that reduced by half the growth of MDCK cells was of 36%. This inhibitory activity was associated with casein and not the whey fraction. Great variability was found in the degree of inhibitory activity depending on the milk sample. The susceptibility of Caco-2 cells to milk inhibitory activity was lower than that of MDCK. Bovine milk did not have any effect on cell growth, either as skimmed milk or as whey or casein. Morphology of cells incubated with active human casein showed abnormal features, such as chromatin condensation, reduced cellular volume and apoptotic bodies, and also fragmented DNA, which are all features of apoptosis.

Keywords

Human milkbovine milkgrowth inhibitionMDCKCaco-2apoptosis
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 3 , August 2009 , pp. 308 - 316
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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