Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T06:52:38.541Z Has data issue: false hasContentIssue false
  • English
  • Français

Conjugated linoleic acid in ewe milk fat

Published online by Cambridge University Press:  23 May 2005

Pilar Luna ,
Javier Fontecha ,
Manuela Juárez  and
Miguel Angel de la Fuente
Pilar Luna
Affiliation:
Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain
Javier Fontecha
Affiliation:
Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain
Manuela Juárez
Affiliation:
Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain
Miguel Angel de la Fuente
Affiliation:
Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain
Get access Rights & Permissions [Opens in a new window]

Abstract

Ewe milk fat from five different herds was studied to determine the content of conjugated linoleic acid (CLA) isomers. Research was carried out by combining gas chromatography-mass spectrometry (GC-MS) of fatty acid methyl esters (FAME) and 4,4-dimethyloxazolyne derivatives (DMOX) with silver ion-high performance liquid chromatography (Ag+-HPLC). Reconstructed mass spectral profiles of CLA characteristic ions from DMOX were used to identify positional isomers and Ag+-HPLC to quantify them. Total CLA content varied from 0·57 to 0·97 g/100 g of total fatty acids. FAME and DMOX were separated into a complex mixture of minor isomers and major rumenic acid (9-cis 11-trans C18:2) by GC-MS using a 100-m polar capillary column. Rumenic acid would represent more than 75% of total CLA. 11-trans 13-trans, 11–13 cis/trans plus trans/cis and 7–9 cis/trans plus trans/cis were the main CLA isomers after rumenic acid. Minor amounts of 8–10 and 10–12 C18:2 isomers were also found. Although most of the isomers were present in each herd's milk, differences in content were observed for some CLA species.

Keywords

EwemilkCLAfatty acidGC-MSDMOXAg+-HPLC
Type
Research Article
Information
Journal of Dairy Research , Volume 72 , Issue 4 , November 2005 , pp. 415 - 424
Copyright
Proprietors of Journal of Dairy Research 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)