Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-27T07:13:52.221Z Has data issue: false hasContentIssue false
  • English
  • Français

Determination of bovine lactoferrin in lactoferrin-supplemented dairy products and raw milk by an automated latex assay

Published online by Cambridge University Press:  21 September 2006

Takashi Soejima ,
Koji Yamauchi ,
Tomoko Yamamoto ,
Yasuko Ohara ,
Eiji Nagao ,
Keiichi Kanbara ,
Munetoshi Fujisawa ,
Yuko Okuda  and
Shyunji Namba
Takashi Soejima
Affiliation:
Analytical Research Center, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Koji Yamauchi
Affiliation:
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Tomoko Yamamoto
Affiliation:
Analytical Research Center, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Yasuko Ohara
Affiliation:
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Eiji Nagao
Affiliation:
Analytical Research Center, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Keiichi Kanbara
Affiliation:
Bio Links Co. Ltd, 18-6, Takayama, Tuzuki-ku, Yokohama, Kanagawa 224-0065
Munetoshi Fujisawa
Affiliation:
Bio Links Co. Ltd, 18-6, Takayama, Tuzuki-ku, Yokohama, Kanagawa 224-0065
Yuko Okuda
Affiliation:
Department of Laboratory Medicine, Toho University Ohmori Hospital, 6-11-1, Ohmorinishi, Ohta-ku, Tokyo 143-8541, Japan
Shyunji Namba
Affiliation:
Department of Laboratory Medicine, Toho University Ohmori Hospital, 6-11-1, Ohmorinishi, Ohta-ku, Tokyo 143-8541, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

Latex immune agglutination method with a multipurpose auto-analyser (the automated latex assay) was validated for determination of bovine lactoferrin (BLF) in various dairy products. Reproducibility-within-laboratory (intermediate precision) due to day for infant formula, UHT milk and yogurt supplemented with BLF at 50 mg/100 g for infant formula and UHT milk, and at 100 mg/100 g for yogurt were 1·62 to 3·10 mg/100 g. Reproducibility-within-laboratory due to analysis (morning, noon, and evening) for raw milk was 1·59 mg/100 g. Trueness, accuracy in determining known amounts added for BLF-supplemented dairy products was −4·7 to −2·0 mg/100 g. BLF concentration in raw milks was 20·3 to 21·8 mg/100 g. Although interference by the matrixes took place in infant formula and raw milk, BLF assays were accurately carried out by 2000-fold dilution or standard-addition method. Automated latex assay for BLF is simple, rapid, precise and accurate enough for a routine method in various dairy products containing BLF.

Keywords

Automated latex assaybovine lactoferrindairy productsdeterminationICHlatex agglutinationvalidation
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 1 , February 2007 , pp. 100 - 105
Copyright
Proprietors of Journal of Dairy Research 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)