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Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Rosalba Mauriello ,
Luigi Moio ,
Nunziatina Intorcia  and
Francesco Addeo
Lina Chianese
Affiliation:
Istituto di Imhistrie Agrarie, Università di Napoli ‘Federico II’, Parco Gussone, 80055 Portici (NA), Italia
Rosalba Mauriello
Affiliation:
Istituto di Imhistrie Agrarie, Università di Napoli ‘Federico II’, Parco Gussone, 80055 Portici (NA), Italia
Luigi Moio
Affiliation:
Istituto di Imhistrie Agrarie, Università di Napoli ‘Federico II’, Parco Gussone, 80055 Portici (NA), Italia
Nunziatina Intorcia
Affiliation:
Istituto di Imhistrie Agrarie, Università di Napoli ‘Federico II’, Parco Gussone, 80055 Portici (NA), Italia
Francesco Addeo
Affiliation:
Istituto di Imhistrie Agrarie, Università di Napoli ‘Federico II’, Parco Gussone, 80055 Portici (NA), Italia
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Summary

Discontinuous PAGE at alkaline and acid pH, polyacrylamide gel isoelectric focusing, two dimensional electrophoresis and immunoblotting have been used to study the heterogeneity of sheep caseins. Three main phenotypes were selected either because of mobility at alkaline and acid pH of the individual αs components or because of their relative intensity. On electrophoresis at alkaline pH, one phenotype showed two distinct bands of lower electrophoretic mobility than β,- and β2-caseins. A detailed study of these components using immunospecific detection with β-casein antiserum showed that these minor components of ovine casein may have a polypeptide chain similar to that of β1- and β2-caseins. Complete electrophoretic patterns of the casein components in some individual milks are also presented.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 1 , February 1992 , pp. 39 - 47
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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References

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