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Development and validation of a nephelometric immunoassay for IgG1 in milk

Published online by Cambridge University Press:  17 June 2002

ROGER COLLIN ,
COLIN PROSSER ,
ROB MCLAREN ,
MARY THOMSON  and
DAVID MALCOLM
ROGER COLLIN
Affiliation:
AgResearch, Ruakura Agricultural Centre, Private Bag 3123, Hamilton, New Zealand
COLIN PROSSER
Affiliation:
AgResearch, Ruakura Agricultural Centre, Private Bag 3123, Hamilton, New Zealand
ROB MCLAREN
Affiliation:
AgResearch, Ruakura Agricultural Centre, Private Bag 3123, Hamilton, New Zealand
MARY THOMSON
Affiliation:
SAITL Dairy Laboratory, PO Box 10208, Hamilton, New Zealand
DAVID MALCOLM
Affiliation:
SAITL Dairy Laboratory, PO Box 10208, Hamilton, New Zealand
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Abstract

A nephelometric immunoassay, with a detection range of 0·3 to 5 g IgG1/l, was developed for the determination of immunoglobulin in bovine milk. The assay exhibited no significant cross-reactivity with αS1-casein, αS2-casein, β-casein, κ-casein or β-lactoglobulin and 39% cross-reactivity with IgG2. The nephelometric assay was compared with ELISA and RID (24 h and 48 h incubations) assays using 105 duplicate milk samples covering IgG1 values ranging from 0·45 to 1·8 g1. The results obtained from all assays showed good agreement with the exception of those obtained by the RID assay (24 h incubation) which gave lower results in samples containing more than 1·2 g IgG1/l. It was concluded that the nephelometric assay is a reliable, rapid and convenient method suitable for the quantification of IgG1 in milk. The assay can be configured for routine high-throughput milk quality assurance for IgG1 in dairy laboratories.

Keywords

BovinecolostrumimmunoassaynephelometricRID
Type
Research Article
Information
Journal of Dairy Research , Volume 69 , Issue 1 , February 2002 , pp. 27 - 35
Copyright
Proprietors of Journal of Dairy Research 2002

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